Combined venom profiling and cytotoxicity screening of the Radde's mountain viper (Montivipera raddei) and Mount Bulgar Viper (Montivipera bulgardaghica) with potent cytotoxicity against human A549 lung carcinoma cells

Nalbantsoy, Ayşe; Hempel, Benjamin-Florian; Petras, Daniel; Heiss, Paul; Göçmen, Bayram; İğci, Naşit; Yıldız, Mehmet Zülfü; Süssmuth, Roderich D.

doi:10.1016/j.toxicon.2017.06.008

Cited by 39 publications

(50 citation statements)

References 42 publications

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“…The use of reporter arrays showed the venom altered the expression of nuclear factor κβ (NF-κB) signalling pathway genes that have a role in inflammatory diseases and cancer [ 69 ]. Furthermore, a combined cytotoxicity screening and venom profiling approach on snake venom revealed the presence of activity against a human lung carcinoma cell line, and identification of a range of proteins including phospholipases and serine proteases [ 70 ].…”

Section: Venomicsmentioning

confidence: 99%

Venomics: A Mini-Review

Wilson

Daly

2018

High-Throughput

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Venomics is the integration of proteomic, genomic and transcriptomic approaches to study venoms. Advances in these approaches have enabled increasingly more comprehensive analyses of venoms to be carried out, overcoming to some extent the limitations imposed by the complexity of the venoms and the small quantities that are often available. Advances in bioinformatics and high-throughput functional assay screening approaches have also had a significant impact on venomics. A combination of all these techniques is critical for enhancing our knowledge on the complexity of venoms and their potential therapeutic and agricultural applications. Here we highlight recent advances in these fields and their impact on venom analyses.

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Section: Venomicsmentioning

confidence: 99%

Venomics: A Mini-Review

Wilson

Daly

2018

High-Throughput

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“…Recently described methods for the proteomic analysis of snake venoms include the “intact mass profiling”, which directly separate the components out of the crude venom without any previous fractionation. The power of this tool has already been demonstrated at the example of whole snake venom proteomes [ 14 , 15 ]. The intact mass analysis of native proteins compared to chemically reduced proteins allows for a classification, based on the number of existing intra- and intermolecular disulfide bonds, and represents an important characteristic of different viper-venom protein families [ 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Venomics of the Vipera ammodytes transcaucasiana and Vipera ammodytes montandoni from Turkey Provides Insights into Kinship

Hempel

Damm

Göçmen

et al. 2018

Toxins

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The Nose-horned Viper (Vipera ammodytes) is one of the most widespread and venomous snakes in Europe, which causes high frequent snakebite accidents. The first comprehensive venom characterization of the regional endemic Transcaucasian Nose-horned Viper (Vipera ammodytes transcaucasiana) and the Transdanubian Sand Viper (Vipera ammodytes montandoni) is reported employing a combination of intact mass profiling and bottom-up proteomics. The bottom-up analysis of both subspecies identified the major snake protein families of viper venoms. Furthermore, intact mass profiling revealed the presence of two tripeptidic metalloprotease inhibitors and their precursors. While previous reports applied multivariate analysis techniques to clarify the taxonomic status of the subspecies, an accurate classification of Vipera ammodytes transcaucasiana is still part of the ongoing research. The comparative analysis of the viper venoms on the proteome level reveals a close relationship between the Vipera ammodytes subspecies, which could be considered to clarify the classification of the Transcaucasian Nose-horned Viper. However, the slightly different ratio of some venom components could be indicating interspecific variations of the two studied subspecies or intraspecies alternations based on small sample size. Additionally, we performed a bioactivity screening with the crude venoms against several human cancerous and non-cancerous cell lines, which showed interesting results against a human breast adenocarcinoma epithelial cell line. Several fractions of Vipera a. transcaucasiana demonstrated a strong cytotoxic effect on triple negative MDA MB 231 breast cancer cells.

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“…The newly developed dot‐ELISA for detection of the venoms of the Indian venomous snakes Naja naja, B. caeruleus, Dabora russelli and Echis carinatus and proteomic enzyme analysis may generally be used to detect scorpion venoms. The venoms of Montivipera raddei and Montivipera bubjardahica , which have high levels of toxicity, have been shown to have potent cytotoxicity against AS49 human lung carcinoma, signifiying that scorpion venom may also have anticancer properties. Lethal doses of L. quinquestriatus were 0.5 mg/kg i.v.…”

Section: Discussionmentioning

confidence: 99%

Determination of median effective dose (ED₅₀) of scorpion antivenom against scorpion envenomation using a newly developed formula

Saganuwan

2018

Anim Models and Exp Med

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Background About 50 species of scorpions cause fatal scorpionism worldwide. Most of these are members of the Buthidae family, and include, among others, Mesobuthus eupeus , Androctonus crassicauda , Leiurus abdullahbayrami , Leiurus quinquestriatus , Tityus pachyurus and Androctonus australis . Because high doses of scorpion venom and antivenom can cause death and hypersensitive reactions, there is a need to develop a formula that can be used to calculate both lethal and effective doses for scorpion venom and antivenom, respectively, thereby obviating the need for laboratory experiments. Methods In view of this, a literature search was carried out with the aim of modifying the formula ( ) for calculation of the median lethal dose (LD 50 ) of scorpion venom and the ED 50 of antivenom. The human equivalent dose (HED) formula was assessed for extrapolation of LD 50 and ED 50 from animals to human for comparison and relevance with the new formula. Results The findings showed that the newly developed formula (LD 50 = ED 50 1/3 × W a × 10 −4 ) yielded results that are very close to the reported values. Therefore, the newly developed and HED formulas can be used for calculation of LD 50 and ED 50 values for scorpion venom and antivenom, respectively. Conclusion The new formula yielded better results than the HED formula, confirming its predictive validity, precision, and reliability, thereby obviating the need for rigorous experiments and justifying the principles of reduction, refinement, and replacement (3Rs).

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Combined venom profiling and cytotoxicity screening of the Radde's mountain viper (Montivipera raddei) and Mount Bulgar Viper (Montivipera bulgardaghica) with potent cytotoxicity against human A549 lung carcinoma cells

Cited by 39 publications

References 42 publications

Venomics: A Mini-Review

Venomics: A Mini-Review

Comparative Venomics of the Vipera ammodytes transcaucasiana and Vipera ammodytes montandoni from Turkey Provides Insights into Kinship

Determination of median effective dose (ED₅₀) of scorpion antivenom against scorpion envenomation using a newly developed formula

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Combined venom profiling and cytotoxicity screening of the Radde's mountain viper (Montivipera raddei) and Mount Bulgar Viper (Montivipera bulgardaghica) with potent cytotoxicity against human A549 lung carcinoma cells

Cited by 39 publications

References 42 publications

Venomics: A Mini-Review

Venomics: A Mini-Review

Comparative Venomics of the Vipera ammodytes transcaucasiana and Vipera ammodytes montandoni from Turkey Provides Insights into Kinship

Determination of median effective dose (ED50) of scorpion antivenom against scorpion envenomation using a newly developed formula

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Determination of median effective dose (ED₅₀) of scorpion antivenom against scorpion envenomation using a newly developed formula