Edamame, or vegetable soybean, has gained popularity in the United States, likely due to its nutritional quality, purported human health benefits, and flavor. Little information is available about the performance of current edamame cultivars in Florida areas. The objective of this study was to evaluate yield and production potential of edamame genotypes in northern Florida. Nineteen genotypes (based on preliminary study) were selected and planted at two locations (Jay and Citra) to evaluate phenotypic performance, including emergence, plant height, nodes per plant, 100-green seed weight, fresh pod yield, percentage of 1-, 2-, and 3-seeded pods, total lipid concentration, and total protein content. Genotypes and locations showed a significant effect on most of the traits studied. Edamame grown at Jay produced significantly higher yield and 100-green seed weight than the plants grown at Citra. Two early maturing genotypes (PI614832 and PI584470) and one late maturing genotype (PI633424) showed high yield potential, >30 g of 100-green seed weight, and high protein content at Jay. In addition, PI417206 showed the highest 100-green seed weight across locations (50.81 g) and demonstrated moderate yield potential with high protein content and low lipid concentration and may be a suitable candidate for cultivation in different areas in Florida after further evaluation. Number of pods per plant and 100-green seed weight significantly correlated with pod yield, could be considered as selection tool for edamame yield under Florida growing conditions. This study has established a basic knowledge on edamame production for Florida growers and selected lines suitable for the region.