Combining mass spectrometry and genetic labeling in mice to report TRP channel expression

Wartenberg, Philipp; Lux, Femke; Busch, Kai; Fecher‐Trost, Claudia; Wyatt, Amanda; Flockerzi, Veit; Krasteva‐Christ, Gabriela; Boehm, Ulrich; Weißgerber, Petra

doi:10.1016/j.mex.2021.101604

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Experimental Design Materials and Methods1

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“…Animals were anesthetized with a mix of ketamine and xylazine and transcardially perfused with PBS followed by ice-cold 4% paraformaldehyde (PFA) in PBS. Organs were dissected as described elsewhere [4] and post-fixed in ice-cold PFA for 3 h. After post-fixation, organs were cryoprotected in 18% sucrose in PBS over night at 4 °C. The nose was decalcified in 0.5 M EDTA for three to four days at 4 °C prior to cryoprotection.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%