Comet assay application in evaluation the safe use of medicinal plants

Mohamed, Ana Mariel; Cangiano, María Alejandra; Alcaráz, Lucía Esther; Satorres, Sara Elena; Laciar, A. L.; Mattana, Claudia Maricel

doi:10.9755/ejfa.2016-06-669

Cited by 8 publications

(4 citation statements)

References 9 publications

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“…Plants have wide extent of pharmacologically convincing phytochemicals. A significant part of them have been represented steady for the treatment of a couple of afflictions of individual, yet couple of phytochemicals like saponin, tannin, cyanogenic glycosides, etc produce ruinous effects after introduction and can go about as ace oxidants, incorporate in all probability responsible for the mutagenicity and genotoxicity [39].…”

Section: Toxicity Studies and Dna Protective Effect Of L Cubeba Extractmentioning

confidence: 99%

RETRACTED: Evaluation of antioxidant activities, toxicity studies and the DNA damage protective effect of various solvent extracts of Litsea cubeba fruits

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Chaudhuri

Pillai

et al. 2020

Heliyon

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Litsea cubeba is devoured by the ethnic individuals of Arunachal Pradesh in India as food and has been traditionally used for curing different ailments. The purpose of present study was to investigate the antioxidant activities of fruits of L. cubeba using different solvent extracts, quantification of phenolics, toxicity studies and DNA damage protective activities. The antioxidant activities of fruits using five different solvent extracts completed utilizing different in vitro examines. The quantitation of phenolic and polyphenolic compounds in the methanol extract of the fruits was carried out by HPLC. The in vitro haemolytic examination of plant concentrates were completed on rat erythrocytes. Appraisal of cytotoxicity of eatable fruits was assessed by MTT measure. The genotoxicity of the contemplated plant was tried by the single-cell gel electrophoresis comet measure. The DNA defensive impacts of the aqueous extracts of fruits on rodent lymphocyte DNA lesions were likewise assessed with the comet test. The extract obtained by methanol exhibited the highest antioxidant activity. The HPLC examination of the methanol concentrate of the plant demonstrated the occurrence of different phenolic acids and flavonoids like caffeic acid (145.96μg/100mg DE), syringic acid (125.85 μg/100mg DE), ferulic acid (155.89 μg/ 100mg DE), apigenin (28.43 μg/100mg DE), kaempferol (53.41 μg/100mg DE) etc. in various amounts. The consequences of haemolytic lethality, cytotoxicity and genotoxicity of fluid concentrates of the edible plant ensure the security at cell and genomic level. The fluid concentrate of the plant fundamentally repressed DNA harm and these information recommend that the watery concentrate of L. cubeba can forestall oxidative DNA harm to rodent lymphocytes, which is likely because of antioxidant constituents in the concentrate. These outcomes demonstrate that L. cubeba can be utilized in dietary applications with a possibility to diminish oxidative pressure.

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Section: Toxicity Studies and Dna Protective Effect Of L Cubeba Extractmentioning

confidence: 99%

RETRACTED: Evaluation of antioxidant activities, toxicity studies and the DNA damage protective effect of various solvent extracts of Litsea cubeba fruits

Seal

Chaudhuri

Pillai

et al. 2020

Heliyon

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“…The alkaline comet assay was selected having in mind that it allows for the sensitive detection of different forms of primary DNA damage in single cells (Rojas et al 1999;Tice et al 2000;Collins 2004;Azqueta et al 2014;Langie et al 2015). Needless to say, more than three decades of use established the method as a useful endpoint in genotoxicity testing of medicinal plants as well as their constituents (Benković et al 2008(Benković et al , 2009Rusak et al 2010;Farhan et al 2015;Azqueta and Collins 2016;Mohamed et al 2016;Qari and El-Assouli 2017). CBMN Cyt assay was selected to obtain more insight into the relationship between primary DNA damage and the cytogenetic outcomes observed following treatments with the tested compounds.…”

Section: Introductionmentioning

confidence: 99%

In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes

et al. 2018

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Strawberry tree (Arbutus unedo L.) leaves have long been used in the traditional medicine of the Mediterranean region. One of their most bioactive constituents is the glycoside arbutin, whose presence makes A. unedo suitable as a potential substitute for bearberry [Arctostaphylos uva ursi (L.) Spreng] leaves, an herbal preparation widely used for treating urinary tract infections. The safety and biocompatibility of strawberry tree water leaf extract have not yet been documented well. This study estimated arbutin content in strawberry tree water leaf extract (STE) using high performance liquid chromatography. Furthermore, we performed an in vitro safety assessment of the 24 h exposure to three presumably non-toxic concentrations of standardized STE and arbutin in human peripheral blood lymphocytes using the apoptosis/necrosis assay, the alkaline comet assay, and the cytokinesis-block micronucleus cytome assay. The STE was also tested for total antioxidant capacity and lipid peroxidation. At a concentration corresponding to the maximum allowable daily intake of arbutin, the tested extract was not cytotoxic, had a negligible potential for causing primary DNA damage and even hindered micronuclei formation in lymphocytes. It also showed a valuable antioxidant capacity, and did not exert marked lipid peroxidation. These promising results represent a solid frame for further development of STE-based herbal preparations. Although arbutin generally had a low DNA damaging potential, the slowing down of lymphocyte proliferation observed after 24 h of exposure points to a cytostatic effect, which merits further research.

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“…Plants contain a wide range of pharmacologically active phytochemicals, some of which have been found to be bene cial in treating various human ailments. However, certain phytochemicals, such as saponins, tannins, and cyanogenic glycosides, have been associated with harmful effects and can contribute to mutagenicity and genotoxicity upon exposure [47].…”

Section: Genotoxicitymentioning

confidence: 99%

Exploring the nutraceutical potential and safety assessment of wild edible plants in Sikkim, India: A comprehensive analysis

Seal

Chaudhuri

Pillai

et al. 2023

Preprint

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The consumption of wild edible plants has been instrumental in addressing food and nutritional needs and improving the health of marginalized communities in rural areas worldwide. This study aimed to assess the nutritive value, mineral content, vitamin content, and toxicological status of ten wild edible plants, namely Aralia armata, Macropanax dispermus, Fagopyrum dibotrys, Heracleum wallichii, Tupistra clarkei, and Rumex nepalensis. These plants are commonly consumed by tribal communities in Sikkim, India. Samples of these plants were collected from various regions in Sikkim, and their nutraceutical potential was evaluated using established food analysis methods recommended by the Association of Official Analytical Chemists. The analysis revealed high protein contents in several species, notably in A. armata (19.30±1.01%). Carbohydrate content ranged from 6.42±0.10% (R. nepalensis) to 11.16±0.48% (T. clarkei). Moreover, all plants exhibited significant mineral content, including sodium, potassium, calcium, iron, zinc, and magnesium. Additionally, water-soluble vitamins such as vitamin C (11.51±0.24 - 87.56±0.41 mg/100g of dry plant material) as well as B1, B2, B3, B5, B6, and B9 were present in appreciable amounts. The levels of antinutrients, such as oxalate, phytate, and saponin, as well as heavy metals (lead, chromium, cadmium, and mercury), were found to be below the established toxic thresholds in all plants. Thus, they pose no immediate health risks associated with these compounds. Furthermore, assessments of haemolytic toxicity, cytotoxicity, and genotoxicity of water extracts from the wild edible plants indicated their safety for human consumption. Therefore, this study highlights the potential utilization of wild edible plants as a source of dietary supplements, potentially leading to their commercialization. Understanding consumer perceptions towards wild edible plants in India is crucial in this regard.

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Comet assay application in evaluation the safe use of medicinal plants

Cited by 8 publications

References 9 publications

RETRACTED: Evaluation of antioxidant activities, toxicity studies and the DNA damage protective effect of various solvent extracts of Litsea cubeba fruits

RETRACTED: Evaluation of antioxidant activities, toxicity studies and the DNA damage protective effect of various solvent extracts of Litsea cubeba fruits

In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes

Exploring the nutraceutical potential and safety assessment of wild edible plants in Sikkim, India: A comprehensive analysis

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