Comet assays to assess DNA damage and repair in grass shrimp embryos exposed to phototoxicants

Lee, R; Kim, G.B

doi:10.1016/s0141-1136(02)00128-9

Cited by 31 publications

(9 citation statements)

References 8 publications

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“…DNA damage in a variety of aquatic animals has been associated with reduced growth, abnormal development and reduced survival of embryos, larvae and adults (Steinert, 1999;Lee et al, 2000). In grass shrimp embryos Comet assay was used to provide a sensitive measure of DNA strand breakage exposed to genotoxicants, highway run off sediments, sediments with coal ash and phototoxicants (Lee et al, 2000(Lee et al, , 2008Lee and Kim, 2002;Kim and Lee, 2004). In the concentrations tested cadmium chloride and mercuric chloride induced Comets in 72% and 61% of the cells respectively after an exposure over a period of 4 h. Genotoxicity of cadmium was reported in rat hemispheres and cerebellum (Klimova and Misurova, 2002) in rat Leydig cells (Yang et al, 2003), in P. pugio embryos (Hook and Lee, 2004), in M. edulis (Pruski and Dixon, 2002;Emmanouil et al, 2007) and in L. vannamei (Chang et al, 2009).…”

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confidence: 99%

Application of primary haemocyte culture of Penaeus monodon in the assessment of cytotoxicity and genotoxicity of heavy metals and pesticides

Jose

Puthumana

Mohandas

et al. 2011

Marine Environmental Research

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a b s t r a c tLack of shrimp cell lines has hindered the study of pollutants which adversely affects shrimp health and its export value. In this context a primary haemocyte culture developed from Penaeus monodon was employed for assessing the cytotoxicity and genotoxicity of two heavy metal compounds, cadmium chloride and mercuric chloride and two organophosphate insecticides, malathion and monocrotophos.Using MTT assay 12 h IC 50 values calculated were 31.09 AE 16.27 mM and 5.52 AE 1.16 mM for cadmium chloride and mercuric chloride and 59.94 AE 52.30 mg l À1 and 186.76 AE 77.00 mg l À1 for malathion and monocrotophos respectively. Employing Comet assay, DNA damage inflicted by these pollutants on haemocytes were evaluated and the pollutants induced DNA damage in >60% of the cells. The study suggested that haemocyte culture could be used as a tool for quantifying cytotoxicity and genotoxicity of aquaculture drugs, management chemicals and pollutants.

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confidence: 99%

Application of primary haemocyte culture of Penaeus monodon in the assessment of cytotoxicity and genotoxicity of heavy metals and pesticides

Jose

Puthumana

Mohandas

et al. 2011

Marine Environmental Research

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“…Unrepaired DNA lesions may cause teratogenesis. Stage 7 grass shrimp (Paleomonetes pugio) embryos rapidly repair lesions caused by exposure to a variety of toxicants (Lee & Kim, 2002). Embryos simultaneously exposed to benzo[a] pyrene (BP) and UV can have higher levels of adduct formation than after exposure to either genotoxicant alone, and these lesions may persist longer (Arfsten, Schaeffer, & Mulveny, 1996).…”

mentioning

confidence: 99%

Interactive effects of UV, benzo[α] pyrene, and cadmium on DNA damage and repair in embryos of the grass shrimp Paleomonetes pugio

Hook

Lee

2004

Marine Environmental Research

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“…After 4 hrs, subsequent damage induction declines, and this is perceived to be mainly through DNA repair. Similar to pyrene-induced DNA damages in shrimp embryos 29 , light-induced DNA damages in HaCaT keratinocytes by pyrene decrease at prolonged incubation in minimum cell growth medium. While the reported shrimp embryos show DNA repair within 24 h, our study suggests that DNA repair starts as early as 4 h for direct DNA damages (Figure 5).…”

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confidence: 77%

DNA damage and repair of human skin keratinocytes concurrently exposed to pyrene derivatives and UVA light

Fullove

2013

Toxicol. Res.

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Polycyclic aromatic hydrocarbons (PAHs), a class of mutagenic environmental contaminants, insert toxicity through both metabolic activation and light irradiation. Pyrene, one of the most widely studied PAHs, along with its mono-substituted derivatives, 1-amino, 1-bromo, 1-hydroxy, and 1-nitropyrene, were chosen to study the effect of substituents on their phototoxicity, DNA damage and repair. Both alkaline Comet assay, which detects direct DNA damages, and Fpg endonuclease Comet assay, which detects oxidative DNA damages, were conducted at 0, 2, 4, 8, and 24 h of incubation of the cells in minimal growth medium after concomitant exposure to pyrene derivatives and UVA light. All these compounds are photocytotoxic and the phototoxicity is both incubation time and PAH dose dependent; whereas, those without light are not toxic. The LC50 obtained are in the range of 3.5 – 9.3 µM. Cellular DNA damages, both direct and oxidative, are observed immediately after the cells are treated with UVA light and the pyrene derivatives at a concentration of 1.0 µM. The amount of DNA damages (both direct and oxidative) increase from 0 to 4 h of incubation. After 4 hours, subsequent damage induction declines, and this is perceived to be mainly through DNA repair. After longer incubation of 8 h, the damaged cellular DNA start to be repaired, resulting in greatly reduced amount of DNA damages, and the DNA damage reaches the minimum at 24 h of incubation. 1-Amopyrene and 1-hydroxypyrene cause more DNA oxidative damages immediately after the exposure (0 h of incubation), and these damages are repaired within the same timeframe as the other tested compounds. The oxidative DNA damages caused by 1-bromopyrene are repaired starting at 2 h of incubation, earlier than the damages caused by all the other compounds.

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Comet assays to assess DNA damage and repair in grass shrimp embryos exposed to phototoxicants

Cited by 31 publications

References 8 publications

Application of primary haemocyte culture of Penaeus monodon in the assessment of cytotoxicity and genotoxicity of heavy metals and pesticides

Application of primary haemocyte culture of Penaeus monodon in the assessment of cytotoxicity and genotoxicity of heavy metals and pesticides

Interactive effects of UV, benzo[α] pyrene, and cadmium on DNA damage and repair in embryos of the grass shrimp Paleomonetes pugio

DNA damage and repair of human skin keratinocytes concurrently exposed to pyrene derivatives and UVA light

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