Comment on ‘Isolation and detection of steroids from human urine by molecularly imprinted solid-phase extraction and liquid chromatography’ by Gadzala-Kopciuch et al., J. Chromatogr. B 877 (2009), 1177–1184

Bui, Bernadette Tse Sum; Haupt, Karsten

doi:10.1016/j.jchromb.2009.09.048

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…Figure (A−D) shows the normalized chromatograms of the recovered fractions from the NIP and the MIP. As expected, and in contrast to the findings reported by Gadzala-Kopciuch et al, a nonspiked urine sample treated in the same manner presented no detectable testosterone (Figure , E). For validation purposes, this sample was spiked with testosterone and T- d 3 at concentrations of 0.25−100 ng/mL for the determination of linearity.…”

Section: Resultssupporting

confidence: 81%

Toward the Use of a Molecularly Imprinted Polymer in Doping Analysis: Selective Preconcentration and Analysis of Testosterone and Epitestosterone in Human Urine

2010

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A molecularly imprinted polymer (MIP), templated with methyltestosterone, has been synthesized for the cleanup of hydrolyzed urine samples for subsequent testosterone (T) quantification by LC-MS/MS. A concentration of 2 ng/mL testosterone could be quantified after a single step extraction on the MIP. The limit of detection and quantification with the criteria of a signal-to-noise ratio of 3 and 5 were 0.3 and 2 ng/mL, respectively. These values meet the conditions set by the World Anti-Doping Agency for the minimum required performance limits for doping controls, between 2 and 10 ng/mL. Epitestosterone (E) was also separated on this polymer and could be detected at concentrations down to 0.3 ng/mL. The quantification of T and E gives access to the determination of the T/E ratio, essential in doping analysis. Hence, our polymers can offer a more specific extraction procedure, resulting in increased sensitivity with limits of detection 10 times lower than the ones achieved by the standard SPE C(18) sorbents employed in official testing laboratories.

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Section: Resultssupporting

confidence: 81%

Toward the Use of a Molecularly Imprinted Polymer in Doping Analysis: Selective Preconcentration and Analysis of Testosterone and Epitestosterone in Human Urine

2010

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Molecular imprinting science and technology: a survey of the literature for the years 2004-2011

2014

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SummaryHerein we present a survey of the literature covering the development of molecular imprinting science and technology over the years 2004 to 2011. In total, 3779 references to the original papers, reviews, edited volumes and monographs from this period are included, along with recently identified uncited materials from prior to 2004 which were omitted in the first instalment of this series covering the years 1930 to 2003. 1 In the presentation of the assembled references, a section presenting reviews and monographs covering the area is followed by sections describing fundamental aspects of molecular imprinting including the development of novel polymer formats. Thereafter, literature describing efforts to apply these polymeric materials to a range of application areas is presented. Current trends and areas of rapid development are discussed.2

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Reply to comment on “Isolation and detection of steroids from human urine by molecularly imprinted solid-phase extraction and liquid chromatography” by Tse Sum Bui and Haupt [J. Chromatogr. B 877 (2009) 4180–4181]

Gadzała‐Kopciuch

Ričanyová

Buszewski

2010

Journal of Chromatography B

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Comment on ‘Isolation and detection of steroids from human urine by molecularly imprinted solid-phase extraction and liquid chromatography’ by Gadzala-Kopciuch et al., J. Chromatogr. B 877 (2009), 1177–1184

Cited by 3 publications

References 12 publications

Toward the Use of a Molecularly Imprinted Polymer in Doping Analysis: Selective Preconcentration and Analysis of Testosterone and Epitestosterone in Human Urine

Toward the Use of a Molecularly Imprinted Polymer in Doping Analysis: Selective Preconcentration and Analysis of Testosterone and Epitestosterone in Human Urine

Molecular imprinting science and technology: a survey of the literature for the years 2004-2011

Reply to comment on “Isolation and detection of steroids from human urine by molecularly imprinted solid-phase extraction and liquid chromatography” by Tse Sum Bui and Haupt [J. Chromatogr. B 877 (2009) 4180–4181]

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