Comment on   “Molecular identification of seven new Zygopteran genera from South China through partial cytochrome oxidase subunit I (COI) gene”

Vega-Sánchez, Yesenia Margarita; Lorenzo-Carballa, M. Olalla; Vilela, Diogo Silva; Ferreira, Rhainer Guillermo Nascimento; Koroiva, None Ricardo

doi:10.1016/j.mgene.2020.100759

Cited by 2 publications

(3 citation statements)

References 11 publications

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“…Subsequently, all these results indicate the importance of performing careful morphological analysis (see this kind of problem in [66]). Moreover, as indicated by Koroiva and Kvist [38], it reinforces that the use of the global database does not allow the correct establishment of all molecular identifications to be correct.…”

Section: Discussionmentioning

confidence: 89%

DNA Barcoding and New Records of Odonates (Insecta: Odonata) from Paraíba State, Brazil

2022

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Odonates (Insecta: Odonata) are important insects in the food chains of freshwater environments around the world, being used as a model species for areas of behavior and analysis of environmental quality. In Brazil, especially in the Northeastern region, both knowledge about the distribution and molecular information of odonate species found in the two main biomes of the region is still limited. Aiming to improve these issues, here, we carried out an Odonata survey in two locations and built a DNA barcode database for species from the state of Paraíba. In total, 15 first records were reported for this Brazilian state and 142 specimens from 27 genera and 45 species had their ‘Folmer’ cytochrome c oxidase subunit I (COI) fragment evaluated. The database we generated includes data for 70% of the Odonata species found in Paraíba state. For 16 species, this is the first DNA barcode available in public sequence repositories. Our results demonstrate that using the COI in the regional scale can help identify and delimit those evaluated. Eight species (17%) showed a low percentage of differentiation (<2%) compared to other species currently deposited in the GenBank or BOLD System; nevertheless, we present morphological traits that reaffirm our identifications. Barcode data provide new insights into Neotropical diversity and deliver basic information for taxonomic analyses.

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Section: Discussionmentioning

confidence: 89%

DNA Barcoding and New Records of Odonates (Insecta: Odonata) from Paraíba State, Brazil

2022

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“…The quality of sequence data depends on many factors and mistakes can occur in any step from specimen collection to final data interpretation. As an example, for resolving taxonomic questions in Odonata by means of DNA barcoding, two main pitfalls have been described: (i) inaccuracies in the identification of voucher specimens and (ii) sequencing errors [54,55]. A recent publication showed that sequences deposited at GenBank described as Odonata specimens were actually dipteran DNA [55].…”

Section: Discussionmentioning

confidence: 99%

“…As an example, for resolving taxonomic questions in Odonata by means of DNA barcoding, two main pitfalls have been described: (i) inaccuracies in the identification of voucher specimens and (ii) sequencing errors [54,55]. A recent publication showed that sequences deposited at GenBank described as Odonata specimens were actually dipteran DNA [55]. In another example, Lorenzo-Carballa et al identified in a molecular revision of Ischnura aurora questionable DNA sequences [56].…”

Section: Discussionmentioning

confidence: 99%

The Quality of Sequence Data Affects Biodiversity and Conservation Perspectives in the Neotropical Damselfly Megaloprepus caerulatus

Feindt¹,

Hadrys

2022

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Ideally, the footprint of the evolutionary history of a species is drawn from integrative studies including quantitative and qualitative taxonomy, biogeography, ecology, and molecular genetics. In today’s research, species delimitations and identification of conservation units is often accompanied by a set of—at minimum—two sequence markers appropriate for the systematic level under investigation. Two such studies re-evaluated the species status in the world’s largest Odonata, the Neotropical damselfly Megaloprepus caerulatus. The species status of the genus Megaloprepus has long been debated. Despite applying a highly similar set of sequence markers, the two studies reached different conclusions concerning species status and population genetic relationships. In this study, we took the unique opportunity to compare the two datasets and analyzed the reasons for those incongruences. The two DNA sequence markers used (16S rDNA and CO1) were re-aligned using a strict conservative approach and the analyses used in both studies were repeated. Going step by step back to the first line of data handling, we show that a high number of unresolved characters in the sequence alignments as well as internal gaps are responsible for the different outcomes in terms of species delimitations and population genetic relationships. Overall, this study shows that high quality raw sequence data are an indispensable requirement, not only in odonate research.

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Comment on “Molecular identification of seven new Zygopteran genera from South China through partial cytochrome oxidase subunit I (COI) gene”

Cited by 2 publications

References 11 publications

DNA Barcoding and New Records of Odonates (Insecta: Odonata) from Paraíba State, Brazil

DNA Barcoding and New Records of Odonates (Insecta: Odonata) from Paraíba State, Brazil

The Quality of Sequence Data Affects Biodiversity and Conservation Perspectives in the Neotropical Damselfly Megaloprepus caerulatus

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