Commercial formulation amendment transiently affects the microbial composition but not the biogas production of a full scale methanogenic UASB reactor

Cabezas, Angela; Bovio, Patricia; Etchebehere, Claudia

doi:10.1080/09593330.2019.1600042

Cited by 7 publications

(2 citation statements)

References 36 publications

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“…The relative abundance of B-OTU2 ranged 5.62-17.09%. As the secondarily dominant OTU, it showed 99% similarity to Terrisporobacter petrolearius, a typical fermentative bacterium (Cabezas et al, 2020) utilizing monosaccharide substrates such as glucose, fructose, and maltose, and producing metabolites such as acetic acid and CO 2 (Zhao et al, 2020). The relative abundance of B-OTU2 was generally maintained above 12% after the onset of anaerobic digestion, with three peaks occurring on the 20th, 60th, and 90th day, respectively.…”

Section: Bacterial Community Structure and Relative Abundancementioning

confidence: 97%

Determining the Microbial Source of Methane Production in Anaerobic Digestion Systems Through High-Throughput Sequencing Technology

et al. 2022

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Anaerobic digestion is widely used to simultaneously generate biogas while treating different organic wastes. It is difficult to determine the source of CH4 from the complex microbial community structure using traditional microbiological pure culture techniques. Therefore, this study aimed to elucidate the microbial source of CH4 in low-temperature anaerobic digestion systems using the recently developed high-throughput sequencing technology. Herein, anaerobic digestion microbes were domesticated at 15°C and then inoculated into pig manure-containing raw materials in a batch anaerobic digester to form a low-temperature anaerobic digestion system with fermentation controlled at 15°C. Several analytical approaches including abiotic factor analysis and biotic factor analysis (high-throughput sequencing) were applied to investigate the abiotic factors, bacterial communities, and archaeal communities in the low-temperature anaerobic digestion system. The results showed that: 1) The anaerobic digestion lasted for 120 days, with 68.65 L total gas production and 31.19 L CH4 production. 2) The relative abundances of the primary and secondary dominant bacterial operational taxonomic units ranged from 8.02 to 22.84% and 5.62–17.09%, respectively, with 99% similarities to Clostridium cellulovorans (a typical cellulose- and hemicellulose-degrading bacterium) and Terrisporobacter petrolearius (a representative fermentation bacterium), respectively. Moreover, the relative abundance of the primary dominant methanogenic archaeal operational taxonomic unit ranged from 1.03 to 16.85%, with 98% similarity to Methanobacterium beijingense, which is a typical hydrogenotropic methanogen. Based on the low-temperature CH4-producing metabolic pathways of bacterial and methanogenic operational taxonomic units, Methanobacterium beijingense was found to be the primary functional microbe for CH4 production in the 15°C anaerobic digestion system.

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Section: Bacterial Community Structure and Relative Abundancementioning

confidence: 97%

Determining the Microbial Source of Methane Production in Anaerobic Digestion Systems Through High-Throughput Sequencing Technology

et al. 2022

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“…If they are not treated, they can be discharged arbitrarily, which is extremely harmful to soil, aquatic organisms, human health and crop growth. (Cabezas et al 2020), the treatment of oily wastewater has become an urgent environmental issue in China (Desilva 2015).Various oily wastewater treatment technologies have been developed, the hydrolytic acidification-aerobic biological treatment process is considered an important link in the treatment of oily wastewater, which converts the insoluble organic matter in the original oily wastewater into dissolved organic matter, the soluble organic matter is absorbed and utilized by the hydrolytic acidifying bacteria, which ultimately improves the biodegradability of oily sewage to facilitate subsequent aerobic treatment (Koo et al 2016;Wang et al 2017). The diversity and structure of the microbial flora in the hydrolysis acidification tank has a significant impact on the conversion of the refractory organic matter in the oily sewage into the easily degradable organic matter.…”

Section: Introductionmentioning

confidence: 99%

Change the original microbial community structure in the hydrolysis acidification tank to enhance the COD removal performance of oily wastewater

Zhao

Yang

et al. 2021

Water Science and Technology

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The hydrolysis acidification tank mainly relies on microorganisms to treat oily sewage, but in many cases the COD of the effluent from the hydrolysis acidification tank does not decrease or even increase. In this paper, about 50 L of oily wastewater is treated in a facultative anaerobic hydrolysis acidification tank with a temperature of 29 °C, pH 6, high-throughput sequencing technology analyzes found that after long-term operation of the hydrolysis and acidification tank, the dominant bacterial Pseudomonas accounted for only 2.87%, at this time, the effluent COD of the hydrolysis and acidification tank was 450 mg/L. Pseudomonas stutzeri LH-42 a strain screened in the laboratory, which was domesticated and colonized in the hydrolysis acidification tank. High-throughput sequencing and bioinformatics analysis showed that the proportion of Pseudomonas in the hydrolysis acidification tank reached 5.89%, the effluent COD of the hydrolysis and acidification tank was 200 mg/L. The above results indicate the importance of the proportion of Pseudomonas in the hydrolysis and acidification tank to the COD degradation of oily wastewater.

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Microbiota adaptation after an alkaline pH perturbation in a full-scale UASB anaerobic reactor treating dairy wastewater

Callejas

Fernandez

Passeggi

et al. 2019

Bioprocess Biosyst Eng

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Commercial formulation amendment transiently affects the microbial composition but not the biogas production of a full scale methanogenic UASB reactor

Cited by 7 publications

References 36 publications

Determining the Microbial Source of Methane Production in Anaerobic Digestion Systems Through High-Throughput Sequencing Technology

Determining the Microbial Source of Methane Production in Anaerobic Digestion Systems Through High-Throughput Sequencing Technology

Change the original microbial community structure in the hydrolysis acidification tank to enhance the COD removal performance of oily wastewater

Microbiota adaptation after an alkaline pH perturbation in a full-scale UASB anaerobic reactor treating dairy wastewater

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