Common bunt resistance gene Bt10 located on wheat chromosome 6D

Abstract

. 2006. Common bunt resistance gene Bt10 located on wheat chromosome 6D. Can. J. Plant Sci. 86: 1409-1412. Knowledge of the chromosomal location of disease resistance genes assists in their identification and classification. The determination of the chromosomal location in wheat of the common bunt (Tilletia tritici and T. laevis) resistance gene Bt10 was the goal of this study. Doubled haploid lines were developed from a cross between bunt susceptible Glenlea and bunt resistant AC Taber carrying Bt10. The doubled haploid lines were inoculated with T. tritici race T19, grown in a growth room and rated for bunt near maturity. A series of 50 wheat microsatellite markers were tested on DNA of the individual lines. The population segregated 1:1 for bunt reaction with clear separation between resistant and susceptible classes. A trait related DNA polymorphism generated by gwm469 located in chromosome 6D fit a 1:1 segregation. Combined segregation of bunt resistance and the gwm469 polymorphism differed significantly from a 1:1:1:1 ratio with a preponderance of parental types confirming linkage of gwm469 with Bt10. The map distance between gwm469 and Bt10 was estimated at 19.3 cM by MAPMAKER. The microsatellite markers wmc749, barc54 and cfd0033, located on chromosome 6D, also were significantly associated with the bunt resistance and gwm469. In total, six markers previously located to chromosome 6D were in the linkage group with the Bt10 common bunt resistance. The linkage of these markers with each other and Bt10 indicated that Bt10 is located on the short arm of chromosome 6D. Savoir où les gènes de résistance à la maladie se situent sur les chromosomes en facilite l'identification et la classification. Les auteurs voulaient établir l'emplacement du gène de résistance à la carie (Tilletia caries et T. laevis) Bt10 sur les chromosomes du blé. Pour cela, ils ont créé des lignées dihaploïdes en croisant la lignée Glenlea, sensible à la carie, avec AC Taber, lignée résistante portant le gène Bt10. Ils ont ensuite inoculé la race T19 de T. caries aux lignées dihaploïdes après culture dans une chambre de croissance et évaluation de sa maturité. Une série de 50 microsatellites ont été testés comme marqueurs avec l'ADN de chaque lignée. La population a donné une ségrégation de 1:1 pour la réaction à la carie avec une nette démarcation entre les sujets résistants et les sujets sensibles. Le polymorphisme de l'ADN engendré par le gène gwm469 sur le chromosome 6D donne lui aussi une ségrégation de 1:1. La ségrégation combinée de la résistance à la carie et du polymorphisme codé par gwm469 donne un ratio sensiblement différent de 1:1:1:1, la prépondérance des caractères parentaux confirmant la liaison génétique entre gwm469 et Bt10. Le logiciel MAPMAKER estime la distance entre les deux gènes à 19,3 cM. Les microsatellites wmc749, barc54 et cfd0033, également situés sur le chromosome 6D, se caractérisent aussi par une association sensible avec la résistance à la carie et gwm469. Dans l'ensemble, six marqueurs qu'on avait antérie...

A novel QTL associated with dwarf bunt resistance in Idaho 444 winter wheat

“…The two studies also used different inoculum sources: the Blizzard study used the common bunt races T-19 or T-19 and L-16, while this study used the composite dwarf bunt races. Additional common bunt HPR QTL have been reported from other resistance sources, including: 7A (Fofana et al 2008; Dumalasová et al 2012), 7B (Dumalasová et al 2012; Knox et al 2013), 5B (Dumalasová et al 2012; Singh et al 2016), 4D (Singh et al 2016), and 6D (Menzies, et al 2006; Singh et al 2016), while none of these HPR QTL were detected in the present study. Singh et al (2016) found that the common bunt HPR QTL co-localized with other beneficial traits including height and rust resistance.…”

“…However, that haplotype is also present in a number of additional lines with known putative resistance genes and also present in the differential lines for Bt5 , Bt8 , Bt9 , Bt10 , Bt12 , and Bt13 (Table 5). It is possible that the QTL contains one or more of these known HPR genes other than Bt10 , as Bt10 is already mapped to chromosome 6D (Menzies et al 2006). The IDO444 haplotype is present in Promontory, while absent in Manning, Deloris, and Utah-100, and these cultivars all have the same putative HPR gene combination including Bt - 3 , Bt - 9 , and Bt - 10 (Table 5).…”

“…One QTL on 1BS for common bunt was reported in two spring wheats, AC Domain (Fofana et al 2008) and Carberry (Singh et al 2016), a US winter wheat Blizzard (Wang et al 2009), and a European winter wheat Trintella (Dumalasová et al 2012). Additional QTL were reported on 7A (Fofana et al 2008; Dumalasová et al 2012), 7B (Dumalasová et al 2012; Knox et al 2013), 5B (Dumalasová et al 2012; Singh et al 2016), 4D (Singh et al 2016), 6D (Menzies, et al 2006; Singh et al 2016), and 7D (Singh et al 2016). Markers for Bt10 on 6D have also been developed (Laroche et al 2000; Menzies et al 2006).…”

Location of genes for common bunt resistance in the European winter wheat cv. Trintella

“…Bt7 (Sel. 50077) has been located on chromosome 2D by Metzger (after McIntosh et al 1998), and Bt10 (PI 178383 9 Elgin) on chromosome 6D (Menzies et al 2006). Recently two papers appeared that attributed common bunt resistance to chromosome 1B (Fofana et al 2008;Wang et al 2009).…”

Population Structure and Genomewide Association Analysis of Resistance to Disease and Insensitivity to Ptr Toxins in Canadian Spring Wheat Using 90K SNP Array

“…Tilletia caries) and Tilletia laevis (syn. Both the incidence and severity of common bunt have been controlled in the Canadian prairies, largely by introgressing resistance genes such as Bt10 and Bt8 (Menzies et al, 2006;McCallum and DePauw, 2008;Hiebert et al, 2011). It reduces both grain yield and quality through the formation of bunt balls that replace the grain with brown black that results in unpleasant-smelling spores (Martens et al, 1984).…”