Common Cell-Surface Antigen Associated with Murine and Feline C-Type RNA Leukemia Viruses

Yoshiki, Takashi; Mellors, Robert C.; Hardy, William

doi:10.1073/pnas.70.6.1878

Cited by 30 publications

(15 citation statements)

References 23 publications

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“…This antigen is distinct from Moloney murine leukemia virus antigens (66,67) and can also exist as a precursor containing gag proteins (68). In addition to Abelson antigen and the cell surface antigen, other transformation-(69) and tumor-associated (70,71) common antigens may determine host defense mechanisms. Finally, virus production may be an important factor in tumor immunity.…”

Section: Methodsmentioning

confidence: 99%

Malignancies of metastatic murine lymphosarcoma cell lines and clones correlate with decreased cell surface display of RNA tumor virus envelope glycoprotein gp70.

Reading¹,

Brunson²,

Torrianni³

et al. 1980

Proc. Natl. Acad. Sci. U.S.A.

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Metastasis is dependent on both host and tumor cell characteristics (1-3). In order to study tumor cell properties associated with metastasis, a number of animal tumor models have been developed by sequential selection methods in vivo (4-9) and in vitro (10-13). Of particular interest are cell surface properties of these tumor models because in several systems the cell surface appears to be important in determining the degree and location of experimental metastasis (3,6,(12)(13)(14)(15)(16).A tumor model for lymphosarcoma metastasis has been developed from the murine RAW117 lymphosarcoma line which is capable of forming some solid tumor nodules in livers, lungs, and spleen of syngeneic BALB/c mice (5). By selecting 10 times for liver colonization, a more malignant lymphosarcoma subline RAW117-HlO was obtained which rapidly killed its host (5) and formed t200-250 times more gross liver tumor nodules than did the parental line RAW117-P after intravenous injection (5, 13). Utilizing the low-malignancy RAW117-P parental line, we selected in vitro for nonadherence to immobilized lectins such as concanavalin A (Con A) to obtain variant sublines that show increased potential for experimental metastasis in vivo (13,17). Similarly, selecting in vitro of the highly malignant subline RAW117-H1O on wheat germ agglutinin (WGA) yielded variant sublines with decreased metastatic potential (13). The cell surface properties of these lymphosarcoma sublines and several clones derived from them have been analyzed, and we find that there is an inverse relationship between the metastatic potentials of these sublines and clones and the expression of cell surface RNA tumor virus envelope glycoprotein gp7O. MATERIALS AND METHODSCell Lines. RAW117 lymphosarcoma cells were grown as described (5). The methods of sequential in vivo selection for malignant variant sublines (RAW117H1 through RAW117-H10) with enhanced ability to form solid tumor colonies in the livers of syngeneic BALB/c mice (5) and the methods for repeated in vitro selections for lectin-binding variant sublines (RAW117-P Con Aa10 and RAW117-H10 WGAal1) on lectincoated petri dishes have been reported (13,17).Cell Clones. Clones were obtained from the established parental cell line RAW117-P, from the in vivo-selected subline RAW117-H10, and from the in vitro-selected sublines RAW117-P Con Aa10 and RAW117-H10 WGAal1 by the limiting-dilution technique. Briefly, cells were diluted to one cell per ml in Dulbecco's modified Eagle's medium (GIBCO) containing 10% heat-inactivated (560C, 30 min) fetal bovine serum (Flow Laboratories, Inglewood, CA), and 0.2 ml was distributed to each of 96 wells in a Microtest II plate (Falcon). After 2 weeks, clones were visible as a small white spot on the bottom of the well; infrequent wells with two clones were discarded. Cloning efficiencies of the RAW117 lines and sublines were approximately 80%In Vivo Assays. RAW1 17 lines were assayed for organ colonization (experimental metastasis) after intravenous injection of 5000 viable lymphosarc...

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Section: Methodsmentioning

confidence: 99%

Malignancies of metastatic murine lymphosarcoma cell lines and clones correlate with decreased cell surface display of RNA tumor virus envelope glycoprotein gp70.

Reading¹,

Brunson²,

Torrianni³

et al. 1980

Proc. Natl. Acad. Sci. U.S.A.

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“…In the present study, target cells were washed 4-5 times before use in the cytotoxic assay and still the antigenic determinants of p30 were detectable as cytotoxic targets. Furthermore, Yoshiki et al (1973Yoshiki et al ( , 1974 have demonstrated with immunoelectron microscopic techniques that p30 is located on cell membranes near the site of virus budding but at a locations distinct from that of VEA. They then showed with immunofluorescence that an antigenic determinant of p30 can actually cap with monospecific antisera, thus indicating an intimate relationship of the p30 molecule with the fluid membrane of C-type RNA virus infected cells.…”

Section: Absorption Studies With Purified Viral Proteinsmentioning

confidence: 99%

“…They suggested that the common cell surface antigen which they detected with this antiserum was p30, since this antigen was the only subviral component to absorb the antibody activity against the common cell surface antigen. In further immunofluorescence studies both the gs1 and gs3 antigenic determinants were detected on the surface of leukaemic cells and tissue culture lines infected with C type RNA viruses (Yoshiki et al, 1974).…”

mentioning

confidence: 92%

“…The gs determinants have been further subdivided into a species specific (gsl) and an interspecies specific determinant (gs3) (Gilden et al, 1971). Yoshiki, Mellors and Hardy (1973), using indirect membrane immunofluorescence, detected a common cell surface antigen associated with murine and feline C type RNA leukaemia viruses in cells infected or transformed by such viruses. In these studies the authors employed an antiserum prepared by the immunization of rabbits with ether disrupted feline leukaemia virus (FeLV).…”

mentioning

confidence: 99%

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Studies on mouse Moloney virus induced tumours: I. The detection of p30 as a cytotoxic target on murine Moloney leukaemic spleen cells, and on an in vitro Moloney sarcoma line by antibody mediated cytotoxicity

Epstein¹,

Knight²

1975

Br J Cancer

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Antigenic determinants of p30, the most abundant internal virion protein of C type RNA viruses, were detected on the surface of spleen cells from mice bearing Moloney leukaemia and on an in vitro line of Moloney sarcoma, MSC. On both cell types, these determinants on the p30 molecules served as cytotoxic targets in a xenogenic complement dependent antibody mediated 51Cr release assay. Two antisera were used: a rat anti MLV -M induced lymphoma serum, and an antiserum raised in goats to either disrupted FeLV. The cytotoxic target antigens of these antisera were analysed by inhibition of cytotoxicity with viral and cellular proteins.

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“…Recent work has suggested that certain of the type C virus structural proteins (p3o, gp7o and plo) can be detected on the external cell surface of MuLV infected cells (Yoshiki, Mellors & Hardy, 1973;Grant et al 1974;Yoshiki et al I974)-The mode of origin of these virus proteins is unclear to date and could be derived from at least three possible events: (I) the insertion of these proteins in the membrane as a preliminary event to virus assembly on the cell membrane; (2) the adsorption of virus proteins to cell surfaces from ruptured viruses released in the culture milieu; (3) the placement of virus proteins on cell surfaces as a by-product of virus infection. We have employed a radioisotopic paired label assay for the study of virus associated surface antigens.…”

mentioning

confidence: 99%

Expression of Virus Structural Proteins on Murine Cell Surfaces in Association with the Production of Murine Leukaemia Virus

O’Brien

Simonson

Boone

1976

Journal of General Virology

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SUMMARYWe have used a quantitative radiolabelled antibody procedure to measure the amount of certain virus structural antigens on the surface of BALB/c RAG cells producing endogenous B-tropic type C virus. RAG cells expressed group specificities of MuLV p3o on their cell surface but did not express gp7o group specificities. However, type specificities of gp7o were expressed on BALB/c cell lines infected with Moloney leukaemia virus. The majority of p3o antigens detected on the RAG cell surface were removed by trypsin and their reappearance was prevented by cycloheximide, even in the presence of' conditioned medium' containing MuLV. Passive adsorption of exogenous MuLV p3o to the surface of virus negative BALB/c fibroblasts reached a maximum of 2o ~ of the protein detectable on virus producing RAG cells. These data support the hypothesis that much, but not all, of the surface p3o is expressed de novo on the cell membrane and not derived from passive adsorption of p3o released from shed virus or as a by-product of virus infection of a cell.

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Common Cell-Surface Antigen Associated with Murine and Feline C-Type RNA Leukemia Viruses

Cited by 30 publications

References 23 publications

Malignancies of metastatic murine lymphosarcoma cell lines and clones correlate with decreased cell surface display of RNA tumor virus envelope glycoprotein gp70.

Malignancies of metastatic murine lymphosarcoma cell lines and clones correlate with decreased cell surface display of RNA tumor virus envelope glycoprotein gp70.

Studies on mouse Moloney virus induced tumours: I. The detection of p30 as a cytotoxic target on murine Moloney leukaemic spleen cells, and on an in vitro Moloney sarcoma line by antibody mediated cytotoxicity

Expression of Virus Structural Proteins on Murine Cell Surfaces in Association with the Production of Murine Leukaemia Virus

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