Computer-aided instruction (CAI) is a teaching method that uses modern media to present teaching content, optimize classroom teaching structure, and achieve teaching goals. Multimedia technology integrates graphics, text, and sound, with a large amount of information, rich content, and a good way of human-computer interaction. This article aims to study the reform of basketball teaching in colleges and universities from the perspective of basketball culture. This article mainly adopts the method of questionnaire survey and uses techniques such as big data statistics to investigate and analyze the composition of basketball teachers in colleges and universities in a certain province; Understand the situation; analyze and understand the impact of basketball culture on the reform of college basketball teaching; analyze the effect of the composition of college basketball teachers on college basketball teaching reform; and investigate the impact of the integration of basketball culture on students’ basketball interest. The results show that about 70% of students believe that basketball can enhance physical fitness and recreation, while most teachers believe that basketball can enhance students ‘physical fitness, impart students’ skills, and promote physical and mental health. The expectations for leisure, entertainment and heritage culture are less than 20%. From the perspective of the age structure of teachers, the basketball team of colleges and universities shows younger characteristics and teachers less than 40 years old account for more than 70% of the total. 81.0% of college basketball teachers are mainly graduates, but the proportion of basketball teachers with a doctorate is significantly lower. Some colleges and universities do not even have a doctorate. Most of them are graduates and undergraduates. Some colleges and universities still have college students. Therefore, teachers often need to have a certain basic knowledge of basketball professional culture, and they should make full use of basketball sports culture and combine them with each other to cultivate their own basketball interests and promote the reform of basketball sports teaching methods in colleges and universities.