Communication in a Protein Stack: Electron Transfer between Cytochrome <i>c</i> and Bilirubin Oxidase within a Polyelectrolyte Multilayer

Dronov, Roman; Kurth, Dirk G.; Möhwald, Helmuth; Scheller, Frieder W.; Lisdat, Fred

doi:10.1002/ange.200704049

Cited by 14 publications

(11 citation statements)

References 38 publications

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“…Furthermore, electron mediation by cytochrome c has been reported with several other enzymes: laccase, ascorbate oxidase [47,60,61], NADPH cytochrome P450 reductase [62], bilirubin oxidase [29,38], xanthine oxidase [46], among others.…”

Section: Cytochrome Cmentioning

confidence: 96%

“…In the presence of oxygen, an increased cathodic current was observed as a result of the enzymatic reduction of oxygen to water. The catalytic current had a linear dependence on the oxygen present in solution [38]. The same research group has later showed that the BOD/cyt c multilayered biosensor could be formed with different forms of cytochrome c, such as human cyt c and some of its mutant variants.…”

Section: Bilirubin Oxidasementioning

confidence: 97%

“…While some approaches make use of simple methods such as entrapment with dialysis membranes [16,34], others integrate the biosensors components with cross-linking agents or polymeric layers [2,33,[35][36][37]. A common trend is also the use of thiolmodified gold electrodes onto which small cytochromes can be attached and display facile DET [37][38][39][40]. Nevertheless, some of the reviewed works do not constitute integrated biosensors, because at least one of the components of the mediated system (mediator protein or enzyme) is not immobilized on the electrode [27,29,[40][41][42][43].…”

Section: Figmentioning

confidence: 99%

“…In a different approach, a number of protein multilayer designs combining cyt c and different enzymes, including BOD, have been reported [38,[45][46][47]49,50]. The bioelectrodes were built using a layer-by-layer self-assembly method.…”

Section: Bilirubin Oxidasementioning

confidence: 99%

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Small electron-transfer proteins as mediators in enzymatic electrochemical biosensors

Silveira

Almeida

2013

Anal Bioanal Chem

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Electrochemical mediators transfer redox equivalents between the active sites of enzymes and electrodes and, in this way, trigger bioelectrocatalytic redox processes. This has been very useful in the development of the so-called second generation biosensors, where they are able to transduce the catalytic event into an electrical signal. Among other pre-requisites, redox mediators must be readily oxidized/reduced at the electrode surface and easily interact with the biorecognition component. Small chemical compounds (e.g. ferrocene derivatives, ruthenium or osmium complexes and viologens) are frequently used for this purpose, but lately, small redox proteins (e.g. horse heart cytochrome c) have also played the role of redox partners in biosensing applications. In general, the docking between two complementary proteins introduces a second level of selectivity to the biosensor and enlarges the list of compounds targeted for analysis. Moreover, electrochemical interferences are frequently minimized owing to the small overpotentials achieved. This paper aims to provide an overview of enzyme biosensors that are mediated by electron transfer proteins. The article begins with a few considerations on mediated electrochemistry in biosensing 2 systems and proceeds with a detailed description of relevant works concerning the cooperative use of redox enzymes and biological electron donors/acceptors.

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Section: Cytochrome Cmentioning

confidence: 96%

Section: Bilirubin Oxidasementioning

confidence: 97%

Section: Figmentioning

confidence: 99%

Section: Bilirubin Oxidasementioning

confidence: 99%

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Small electron-transfer proteins as mediators in enzymatic electrochemical biosensors

Silveira

Almeida

2013

Anal Bioanal Chem

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“…Recently, BFCs have attracted great interest as the power supplies for a cardiac pacemaker, toys, integrated circuits, or relatively low electrical power devices to keep the environment safe and establish a sustainable society, since the systems operate under mild conditions at around body temperature of 20-40 °C in near-neutral pH as well as natural materials can be utilized in the electrode process [3][4][5][6][7][8][9][10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Biofuel Cells with Hemoglobin as Cathodic Electrocatalysts for Hydrogen Peroxide Reduction on Bare Indium-Tin-Oxide Electrodes

Ayato

Matsuda

2013

Energies

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A biofuel cell (BFC) cathode has been developed based on direct electron transfer (DET) of hemoglobin (Hb) molecules with an indium-tin-oxide (ITO) electrode and their electrocatalysis for reduction of hydrogen peroxide (H 2 O 2 ). In this study, the ITO-coated glass plates or porous glasses were prepared by using a chemical vapor deposition (CVD) method and examined the electrochemical characteristics of the formed ITO in pH 7.4 of phosphate buffered saline (PBS) solutions containing and not containing Hb. In half-cell measurements, the reduction current of H 2 O 2 due to the electrocatalytic activity of Hb increased with decreasing electrode potential from around 0.1 V versus Ag|AgCl|KCl(satd.) in the PBS solution. The practical open-circuit voltage (OCV) on BFCs utilizing H 2 O 2 reduction at the Hb-ITO cathode with a hydrogen (H 2 ) oxidation anode at a platinum (Pt) electrode was expected to be at least 0.74 V from the theoretical H 2 oxidation potential of −0.64 V versus Ag|AgCl|KCl(satd.) in pH 7.4. The assembled single cell using the ITO-coated glass plate showed the OCV of 0.72 V and the maximum power density of 3.1 µW cm −2. The maximum power per single cell was recorded at 21.5 µW by using the ITO-coated porous glass.

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Nanobiomolekulare Multiproteinarchitekturen zur Etablierung von schaltbaren Signalketten auf Elektroden

et al. 2014

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Communication in a Protein Stack: Electron Transfer between Cytochrome c and Bilirubin Oxidase within a Polyelectrolyte Multilayer

Cited by 14 publications

References 38 publications

Small electron-transfer proteins as mediators in enzymatic electrochemical biosensors

Small electron-transfer proteins as mediators in enzymatic electrochemical biosensors

Evaluation of Biofuel Cells with Hemoglobin as Cathodic Electrocatalysts for Hydrogen Peroxide Reduction on Bare Indium-Tin-Oxide Electrodes

Nanobiomolekulare Multiproteinarchitekturen zur Etablierung von schaltbaren Signalketten auf Elektroden

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