Many rural areas of Africa are characterised by high levels of unemployment, poverty and increasing population densities. Arid climates and erratic rainfall also make many of these areas marginal for agriculture and offer few alternative employment opportunities. Ecotourism in these areas can offer a sustainable land use option that promotes biodiversity conservation, can assist in poverty reduction and promote local socio-economic development. Receipt of benefits from ecotourism is also claimed to improve community attitudes towards conservation. Through extensive questionnaire surveys, this study analysed the impact of land management systems on community attitudes towards tourism and conservation in six southern African countries: Botswana, Malawi, Namibia, South Africa, Zambia and Zimbabwe. The study found that diverse land management systems such as government-owned land and communal land had differing effects on attitudes. Overall, some level of community ownership or participation, such as in joint ventures, resulted in more positive community attitudes. The results highlight the importance of empowerment and ownership in order to promote biodiversity conservation and ensure the long-term sustainability of ecotourism operations.