Community level physiological profiles of bacterial communities inhabiting uranium mining impacted sites

Kenarova, Anelia; Radeva, Galina; Traykov, I.; Boteva, Silvena

doi:10.1016/j.ecoenv.2013.11.012

Cited by 94 publications

(57 citation statements)

References 37 publications

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“…Substrate average well color development (SAWCD) values for each substrate categories were calculated with the same equation: SAWCD=∑ODi/N, where ODi is the corrected OD value of the substrates within the substrate category and N is the number of substrates in the category (Kenarova et al, 2014). …”

Section: Methodsmentioning

confidence: 99%

“…The Biolog EcoPlate technique has been increasingly used to assess the toxicological impacts of different pollutants (Preston-Mafham et al, 2002), such as heavy metals (Rusk et al, 2004;Kamitani et al, 2005, Niklinska et al, 2005, Boshoff et al, 2014, uranium (Kenarova et al, 2014) or hydrocarbon contamination (Bundy et al, 2004, Nagy et al, 2013 on soil microbial populations, or for estimating the impact of other stressing factors such as high salinity and high soil pH (Pankhurst et al, 2001), or heating (Pietikäinen et al, 2000).…”

mentioning

confidence: 99%

“…AWCD values can be subdivided into SAWCD (substrate average well color development) groups based on substrate guilds (carbon sources) of similar chemical nature, (e.g. amino acids, amines, carbohydrates, carboxylic acids, phenolyc compounds and polymers) to assess the potential of the soil microbial community to degrade these carbon sources (Zak et al, 1994, Kenarova et al, 2014, Sala et al, 2010. The Shannon diversity index (H) is used to calculate the physiological diversity of bacterial communities (Kenarova et al, 2014).…”

mentioning

confidence: 99%

“…Biolog-derived data for evaluation generally include: the Average Well Colour Development (AWCD) and the Shannon diversity index (H) (Stefanowicz, 2006, Frąc et al, 2012, Kenarova et al, 2014, Muñiz et al, 2014. AWCD is an indicator of the general potential metabolic activity of the microbial community, thus it is an index of the total bioactivity for the Biolog plates (Frąc et al, 2012, Kenarova et al, 2014. AWCD values can be subdivided into SAWCD (substrate average well color development) groups based on substrate guilds (carbon sources) of similar chemical nature, (e.g.…”

mentioning

confidence: 99%

“…amino acids, amines, carbohydrates, carboxylic acids, phenolyc compounds and polymers) to assess the potential of the soil microbial community to degrade these carbon sources (Zak et al, 1994, Kenarova et al, 2014, Sala et al, 2010. The Shannon diversity index (H) is used to calculate the physiological diversity of bacterial communities (Kenarova et al, 2014). Microbial communities that are able to degrade more substrates or/and to degrade them with similar efficiency would have higher values of H compared to that part of the community which is not metabolically active and is not capable of growing in plate conditions (Muñiz et al, 2014).…”

mentioning

confidence: 99%

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Influence of red mud on soil microbial communities: Application and comprehensive evaluation of the Biolog EcoPlate approach as a tool in soil microbiological studies

Feigl

Ujaczki

Vaszita

et al. 2017

Science of The Total Environment

132

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Red mud can be applied as soil ameliorant to acidic, sandy and micronutrient deficient soils. There are still knowledge gaps regarding the effects of red mud on the soil microbial community. The Biolog EcoPlate technique is a promising tool for community level physiological profiling. This study presents a detailed evaluation of Biolog EcoPlate data from two case studies. In experiment "A" red mud from Ajka (Hungary) was mixed into acidic sandy soil in soil microcosms at 5-50 w/w%. In experiement "B" red mud soil mixture was mixed into low quality subsoil in a field experiment at 5-50 w/w%. According to average well color development, substrate average well color development and substrate richness 5-20% red mud increased the microbial activity of the acidic sandy soil over the short term, but the effect did not last for 10 months. Shannon diversity index showed that red mud at up to 20% did not change microbial diversity over the short term, but the diversity decreased by the 10 th month. 30-50% red mud had deteriorating effect on the soil microflora. 5-20% red mud soil mixture in the low quality subsoil had a long lasting enhancing effect on the microbial community based on all Biolog EcoPlate parameters. However, 50% red mud soil mixture caused a decrease in diversity and substrate richness. With the Biolog EcoPlate we were able to monitor the changes of the microbial community in red mud affected soils and to assess the amount of red mud and red mud soil mixture applicable for soil treatment in these cases.

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Section: Methodsmentioning

confidence: 99%