Objective: To investigate the association between consumption of certain foods and macronutrients and urinary glucose excretion, which is a predictor of non-insulin-dependent diabetes mellitus. Design: A cross-sectional study, Denmark, 1993 ± 97. Subjects: Participants in the Danish study`Diet, Cancer and Health'. After exclusion of persons with postprandial urine samples and persons with diabetes or other diseases potentially resulting in glycosuria, the study population included 14 743 men and 18 064 women aged 50 ± 64 y. We identi®ed 183 men and 43 women with glucose in their urine. Results: Consumption of poultry was negatively associated with glycosuria in both men (odds ratio, OR 0.87; 95% con®dence interval, 95% CI 0.77 ± 0.98) and women (OR 0.69; 0.48 ± 1.00). Fiber from fruit showed a weak negative association with glycosuria in both men (0.95; 0.90 ± 1.01) and women (0.89; 0.78 ± 1.02), whereas a signi®cant negative association with total ®ber (0.68; 0.51 ± 0.91) and ®ber from vegetables (0.94; 0.88 ± 0.99) was seen in men. Intake of ®sh tended to reduce the risk of glycosuria in women only (0.80; 0.63 ± 1.02), whereas ingestion of milk products increased their risk signi®cantly (1.15; 1.06 ± 1.24). Conclusion: Although statistical signi®cance and consistency in the two sexes were not achieved for all endpoints, the study indicates a protective effect of dietary products like poultry, fruit and cereals against glycosuria and suggests a promoting effect of milk. Sponsorship: The Danish National Board of Health and the Danish Cancer Society.