Community succession and straw degradation characteristics using a microbial decomposer at low temperature

Borjigin, Qinggeer; Gao, Julin; Yu, Xiang; Zhang, Bizhou; Hu, Shuping; Han, Shengcai; Zhang, Sainan

doi:10.1371/journal.pone.0270162

Cited by 4 publications

(5 citation statements)

References 35 publications

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“…Depending on the design and the duration of the experiment on the straw decomposition, two or three phases could be distinguished in a process of microbial succession [21,22,31]. Our data allowed us to distinguish three phases of bacterial succession during the decomposition of lignocellulosic subtract: early (1st month), middle (2nd month), and late (3rd month).…”

Section: Discussionmentioning

confidence: 93%

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The Succession of the Cellulolytic Microbial Community from the Soil during Oat Straw Decomposition

Kimeklis¹,

Gladkov²,

Orlova³

et al. 2022

Preprint

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The decomposition of straw is a dynamic process, which is reflected in the decomposing microbial consortia, however, this process is poorly understood. Here we performed an experiment on the gradual colonization of oat straw by the soil microbial community. The results showed that soil respiration and bacterial diversity were negatively correlated – while the first decreased, the latter increased. In accordance with the dynamics of diversity and respiration, succession was divided into early, middle, and late decomposition phases. Analysis of 16S rRNA and ITS2 amplicon sequencing data revealed three jointly changing groups of phylotypes, corresponding with distinguished phases of decomposition. The presence of cellulolytic members of Proteobacteria, Bacteroidota, Firmicutes, and Actinobacteriota for bacteria and Ascomycota for fungi had been detected since the early phase, but most of the initial phylotypes were gone by the end of the phase. The second phase marked the functional core with a constant set of other phylotypes from these phyla, persisting in the community. Full metagenome sequencing of the microbial community from the end of the middle phase confirmed that major members of this consortium had GH genes, connected to cellulose and chitin degradation. The appearance of non-cellulolytic members from Bdellovibrio, Myxococcota, Chloroflexi, and Crenarchaeota characterized the last phase of the mature community.

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Section: Discussionmentioning

confidence: 93%

“…Multiple studies have shown that during the composting of untreated straw with a natural epiphytic microbiome, the microbial community undergoes taxonomic and functional succession [21,22]. The aim of this study was to isolate the cellulolytic community from the soil and study its full succession stages in sterile straw.…”

Section: Introductionmentioning

confidence: 99%

The Succession of the Cellulolytic Microbial Community from the Soil during Oat Straw Decomposition

Kimeklis¹,

Gladkov²,

Orlova³

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Depending on the design and the duration of the experiment on the straw decomposition, two or three phases could be distinguished in a process of microbial succession [ 21 , 22 , 35 ]. Our data allowed us to distinguish three phases of bacterial succession during the decomposition of lignocellulosic subtract: early (first month), middle (second to third month), and late (fourth to sixth month).…”

Section: Discussionmentioning

confidence: 99%

“…Multiple studies have shown that during the composting of untreated straw with a natural epiphytic microbiome, the microbial community undergoes taxonomic and functional succession [ 21 , 22 ]. Meanwhile, straw introduction into the soil creates a surplus of nutrients, specifically carbon compounds, which facilitates a new path in the microbiota succession [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

The Succession of the Cellulolytic Microbial Community from the Soil during Oat Straw Decomposition

Kimeklis

Gladkov

Орлова

et al. 2023

IJMS

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The process of straw decomposition is dynamic and is accompanied by the succession of the microbial decomposing community, which is driven by poorly understood interactions between microorganisms. Soil is a complex ecological niche, and the soil microbiome can serve as a source of potentially active cellulolytic microorganisms. Here, we performed an experiment on the de novo colonization of oat straw by the soil microbial community by placing nylon bags with sterilized oat straw in the pots filled with chernozem soil and incubating them for 6 months. The aim was to investigate the changes in decomposer microbiota during this process using conventional sequencing techniques. The bacterial succession during straw decomposition occurred in three phases: the early phase (first month) was characterized by high microbial activity and low diversity, the middle phase (second to third month) was characterized by low activity and low diversity, and the late phase (fourth to sixth months) was characterized by low activity and high diversity. Analysis of amplicon sequencing data revealed three groups of co-changing phylotypes corresponding to these phases. The early active phase was abundant in the cellulolytic members from Pseudomonadota, Bacteroidota, Bacillota, and Actinobacteriota for bacteria and Ascomycota for fungi, and most of the primary phylotypes were gone by the end of the phase. The second intermediate phase was marked by the set of phylotypes from the same phyla persisting in the community. In the mature community of the late phase, apart from the core phylotypes, non-cellulolytic members from Bdellovibrionota, Myxococcota, Chloroflexota, and Thermoproteota appeared. Full metagenome sequencing of the microbial community from the end of the middle phase confirmed that major bacterial and fungal members of this consortium had genes of glycoside hydrolases (GH) connected to cellulose and chitin degradation. The real-time analysis of the selection of these genes showed that their representation varied between phases, and this occurred under the influence of the host, and not the GH family factor. Our findings demonstrate that soil microbial community may act as an efficient source of cellulolytic microorganisms and that colonization of the cellulolytic substrate occurs in several phases, each characterized by its own taxonomic and functional profile.

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“…This is consistent with the results of this study. Temperature has a significant impact on straw decomposition, and the in situ decomposition rate of straw is often slow under low-temperature conditions [36]. It has been reported that the microbial straw decomposition additive can raise the environmental temperature, which improves the decomposition effect of straw [37,38].…”

Section: Effect Of Microbial Agents and Mulch Film Covering On The Ph...mentioning

confidence: 99%

From Residue to Resource: A Physicochemical and Microbiological Analysis of Soil Microbial Communities through Film Mulch-Enhanced Rice Straw Return Strategies

Wang,

Huang,

Yang

et al. 2024

Agronomy

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Promoting rice straw in situ return is an important strategy for improving soil quality. From 2018 to 2021, we investigated the effects of rice straw return with microbial agents and film covering technology on soil physical and chemical properties at different layer depths, as well as the soil microbial community structure, in Hunan, southern China. This study was designed to evaluate the effects of microbial agents (T1), film mulch covering (T2), and the application of microbial agents combined with film mulch (T3) on the soil physicochemical properties and microbial community after rice straw in situ return. The results show that, after three years of continuous treatment, T3 significantly increased the soil temperature by 17.76–22.97%, T2 significantly increased the water content by 34.27–46.23%, and T1 and T3 significantly increased the soil pH. The addition of microbial agents combined with film mulch resulted in a notable increase in both the number of OTUs and the Chao1 index of soil microorganisms. Additionally, the model of promoting rice straw in situ return (the application of a microbial agent combined with film mulch) was shown to promote the growth of beneficial soil microorganisms. RDA was used for the investigation, and the findings showed that soil microorganisms were significantly influenced by the TOC content, pH, and water content. These findings provide evidence of an effective method for accelerating the decomposition of late rice straw and guiding soil improvement in tobacco–rice rotation regions.

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Community succession and straw degradation characteristics using a microbial decomposer at low temperature

Cited by 4 publications

References 35 publications

The Succession of the Cellulolytic Microbial Community from the Soil during Oat Straw Decomposition

The Succession of the Cellulolytic Microbial Community from the Soil during Oat Straw Decomposition

The Succession of the Cellulolytic Microbial Community from the Soil during Oat Straw Decomposition

From Residue to Resource: A Physicochemical and Microbiological Analysis of Soil Microbial Communities through Film Mulch-Enhanced Rice Straw Return Strategies

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