Compact diode laser source for multiphoton biological imaging

Niederriter, Robert D.; Ozbay, Baris N.; Futia, Gregory L.; Gibson, Emily A.; Gopinath, Juliet T.

doi:10.1364/boe.8.000315

Cited by 11 publications

(4 citation statements)

References 38 publications

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“…To determine the orientation of PGS and PLGA within the nanofibers, we developed a method to investigate this using Raman imaging [ 36 ]. Through confocal Raman spectroscopy of single fiber strands, it was previously confirmed that the 1.5/1.5/11/15 core/shell electrospinning configuration obtained specific chemical composition signatures of PGS in the core and PLGA in the shell [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

Mesenchymal Cells Affect Salivary Epithelial Cell Morphology on PGS/PLGA Core/Shell Nanofibers

Sfakis

Kamaldinov

Khmaladze

et al. 2018

IJMS

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Engineering salivary glands is of interest due to the damaging effects of radiation therapy and the autoimmune disease Sjögren’s syndrome on salivary gland function. One of the current problems in tissue engineering is that in vitro studies often fail to predict in vivo regeneration due to failure of cells to interact with scaffolds and of the single cell types that are typically used for these studies. Although poly (lactic co glycolic acid) (PLGA) nanofiber scaffolds have been used for in vitro growth of epithelial cells, PLGA has low compliance and cells do not penetrate the scaffolds. Using a core-shell electrospinning technique, we incorporated poly (glycerol sebacate) (PGS) into PLGA scaffolds to increase the compliance and decrease hydrophobicity. PGS/PLGA scaffolds promoted epithelial cell penetration into the scaffold and apical localization of tight junction proteins, which is necessary for epithelial cell function. Additionally, co-culture of the salivary epithelial cells with NIH3T3 mesenchymal cells on PGS/PLGA scaffolds facilitated epithelial tissue reorganization and apical localization of tight junction proteins significantly more than in the absence of the mesenchyme. These data demonstrate the applicability of PGS/PLGA nanofibers for epithelial cell self-organization and facilitation of co-culture cell interactions that promote tissue self-organization in vitro.

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Section: Resultsmentioning

confidence: 99%

Mesenchymal Cells Affect Salivary Epithelial Cell Morphology on PGS/PLGA Core/Shell Nanofibers

Sfakis

Kamaldinov

Khmaladze

et al. 2018

IJMS

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“…Efficiency of multiphoton processes is influenced by (i) focalization characteristics, (ii) characteristics of the biological sample imaged and (iii) excitation source and its parameters. A theory considered as a reference for the comparison of sources for MPM is precisely described by Webb et al [2,14,51], and is regularly recalled for pulsed lasers [9,16,135,136,138,144], in the case of mode-locked systems or alternative technologies. This evaluation is based either on the estimation of the number of photons emitted by TPF or on the number of photons absorbed by TPA.…”

Section: Theoretical Elements Of Laser Source Comparisonmentioning

confidence: 99%

A review of biomedical multiphoton microscopy and its laser sources

Lefort¹

2017

J. Phys. D: Appl. Phys.

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Multiphoton microscopy (MPM) has been the subject of major development efforts for about 25 years for imaging biological specimens at micron scale and presented as an elegant alternative to classical fluorescence methods such as confocal microscopy. In this topical review, the main interests and technical requirements of MPM are addressed with a focus on the crucial role of excitation source for optimization of multiphoton processes. Then, an overview of the different sources successfully demonstrated in literature for MPM is presented, and their physical parameters are inventoried. A classification of these sources in function with their ability to optimize multiphoton processes is proposed, following a protocol found in literature. Starting from these considerations, a suggestion of a possible identikit of the ideal laser source for MPM concludes this topical review.

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“…Besides, the core diameter of fiber employed in this demonstration was ~21 um, suggesting only ~300 um 2 fundamental mode (FM) field area. Moreover, such long cavity lengths are not desirable for high peak power ultrafast lasers at 976 nm [12,13], which find application in two-photon microscopy [14] and frequency conversion to 488 nm in crystals [13]. To achieve high efficiency ultrafast lasing, it is highly desirable to have an effectively singlemoded, large mode area YDF that inherently possesses high differential loss at four-level lasing wavelengths.…”

Section: Introductionmentioning

confidence: 99%

All-solid antiresonant fiber design for high-efficiency three-level lasing in ytterbium-doped fiber lasers

Goel

Yoo

2022

Opt. Lett.

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We propose and investigate an all-solid ytterbium-doped antiresonant fiber (YbARF) design to inherently suppress four-level lasing with >20 dB/m of selective loss and achieve high-efficiency three-level lasing while maintaining near-diffraction-limited operation with an ultra-large mode area of approximately 3630 µm2. The YbARF is designed such that the high-gain wavelengths corresponding to four-level lasing lie in the resonance band characterized by high confinement loss. This enables three-level lasing with high efficiency in a short (0.8-m-long) YbARF, making it a potential candidate for high-peak-power ultrafast lasers at 976 nm. We discuss fiber design considerations and detailed simulation results for three-level lasing performance in the YbARF, which promises >85% lasing efficiency in a single-pass pump configuration. These design concepts can be easily extended to suppress high-gain wavelengths in other rare-earth-doped (e.g., with thulium, erbium, and neodymium) fiber amplifiers or lasers.

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Compact diode laser source for multiphoton biological imaging

Cited by 11 publications

References 38 publications

Mesenchymal Cells Affect Salivary Epithelial Cell Morphology on PGS/PLGA Core/Shell Nanofibers

Mesenchymal Cells Affect Salivary Epithelial Cell Morphology on PGS/PLGA Core/Shell Nanofibers

A review of biomedical multiphoton microscopy and its laser sources

All-solid antiresonant fiber design for high-efficiency three-level lasing in ytterbium-doped fiber lasers

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