A review of biomedical multiphoton microscopy and its laser sources

Lefort, Claire

doi:10.1088/1361-6463/aa8050

Cited by 94 publications

(65 citation statements)

References 150 publications

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“…OCTA generates angiographic images based on intensity and phase fluctuations of the backscattered light. MPM allows label-free imaging of endogenous fluorophores in skin, such as nicotinamide adenine dinucleotide (phosphate) (NAD(P)H), and flavin adenine dinucleotide (FAD), which allows the investigation of cellular metabolic states 20 21. Additionally, MPM provides reduced tissue photodamage, photobleaching, and phototoxicity 22.…”

Section: Introductionmentioning

confidence: 99%

Non-invasive monitoring of pharmacodynamics during the skin wound healing process using multimodal optical microscopy

Rico-Jimenez

Lee

Alex

et al. 2020

BMJ Open Diab Res Care

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ObjectiveImpaired diabetic wound healing is one of the serious complications associated with diabetes. In patients with diabetes, this impairment is characterized by several physiological abnormalities such as metabolic changes, reduced collagen production, and diminished angiogenesis. We designed and developed a multimodal optical imaging system that can longitudinally monitor formation of new blood vessels, metabolic changes, and collagen deposition in a non-invasive, label-free manner.Research design and methodsThe closure of a skin wound in (db/db) mice, which presents delayed wound healing pathologically similar to conditions in human type 2 diabetes mellitus, was non-invasively followed using the custom-built multimodal microscope. In this microscope, optical coherence tomography angiography was used for studying neovascularization, fluorescence lifetime imaging microscopy for nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) assessment, fluorescence intensity changes of NAD(P)H and flavin adenine dinucleotide (FAD) cofactors for evaluating metabolic changes, and second harmonic generation microscopy for analyzing collagen deposition and organization. The animals were separated into four groups: control, placebo, low concentration (LC), and high concentration (HC) treatment. Images of the wound and surrounding areas were acquired at different time points during a 28-day period.ResultsVarious physiological changes measured using the optical imaging modalities at different phases of wound healing were compared. A statistically significant improvement in the functional relationship between angiogenesis, metabolism, and structural integrity was observed in the HC group.ConclusionsThis study demonstrated the capability of multimodal optical imaging to non-invasively monitor various physiological aspects of the wound healing process, and thus become a promising tool in the development of better diagnostic, treatment, and monitoring strategies for diabetic wound care.

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Section: Introductionmentioning

confidence: 99%

Non-invasive monitoring of pharmacodynamics during the skin wound healing process using multimodal optical microscopy

Rico-Jimenez

Lee

Alex

et al. 2020

BMJ Open Diab Res Care

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“…Recently, an advanced TPFM with a high sampling speed of 2.7 kHz has been developed using a multilfocus compressive sensing method and a digital micromirror device [62]. Besides, the spatiotemporal resolution of TPFM is relatively low, which is theoretically 500 nm laterally and 1 µm axially; it also follows the diffraction limitation [63]. Other studies have proposed a fast high-resolution miniaturized two-photon microscope (FHIRM-TPM) with a lateral resolution of 640 nm and an axial resolution of 3.35 µm [64], and a photomultiplier TPFM with a lateral resolution of 550 nm [65].…”

Section: Two-photon Fluorescence Microscope (Tpfm)mentioning

confidence: 99%

“…Adapted with permission from Reference [47]; (e) Schematic of a typical TPFM. Adapted with permission from Reference [63]; (f) Long-term motility measurement of transferred T cells with TPFM. Adapted with permission from Reference [58]; (g) Basic schematic of LSFM.…”

Section: Light Sheet Fluorescence Microscope (Lsfm)mentioning

confidence: 99%

Advanced Biological Imaging for Intracellular Micromanipulation: Methods and Applications

et al. 2020

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Intracellular micromanipulation assisted by robotic systems has valuable applications in biomedical research, such as genetic diagnosis and genome-editing tasks. However, current studies suffer from a low success rate and a large operation damage because of insufficient information on the operation information of targeted specimens. The complexity of the intracellular environment causes difficulties in visualizing manipulation tools and specimens. This review summarizes and analyzes the current development of advanced biological imaging sampling and computational processing methods in intracellular micromanipulation applications. It also discusses the related limitations and future extension, providing an important reference about this field.

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“…In contrast, the relatively recent development of multi-photon microscopy (MPM) techniques has provided a powerful set of tools for in vivo tissue imaging [2,3]. These non-linear optical modalities can be divided into two classes: Firstly, intensity methods such as two-photon excitation fluorescence (TPEF) [4], second harmonic generation (SHG) [5], and coherent anti-Stokes Raman scattering (CARS) [6].…”

Section: Introductionmentioning

confidence: 99%

Digital staining through the application of deep neural networks to multi-modal multi-photon microscopy

Borhani

Bower

Boppart

et al. 2019

Biomed. Opt. Express

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Deep neural networks have been used to map multi-modal, multi-photon microscopy measurements of a label-free tissue sample to its corresponding histologically stained brightfield microscope colour image. It is shown that the extra structural and functional contrasts provided by using two source modes, namely two-photon excitation microscopy and fluorescence lifetime imaging, result in a more faithful reconstruction of the target haematoxylin and eosin stained mode. This modal mapping procedure can aid histopathologists, since it provides access to unobserved imaging modalities, and translates the high-dimensional numerical data generated by multi-modal, multi-photon microscopy into traditionally accepted visual forms. Furthermore, by combining the strengths of traditional chemical staining and modern multi-photon microscopy techniques, modal mapping enables label-free, non-invasive studies of in vivo tissue samples or intravital microscopic imaging inside living animals. The results show that modal co-registration and the inclusion of spatial variations increase the visual accuracy of the mapped results.

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A review of biomedical multiphoton microscopy and its laser sources

Cited by 94 publications

References 150 publications

Non-invasive monitoring of pharmacodynamics during the skin wound healing process using multimodal optical microscopy

Non-invasive monitoring of pharmacodynamics during the skin wound healing process using multimodal optical microscopy

Advanced Biological Imaging for Intracellular Micromanipulation: Methods and Applications

Digital staining through the application of deep neural networks to multi-modal multi-photon microscopy

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