Compact fluorescence system with fiber-coupled LED for studying photobleaching

Weng, Chun-Jen; Lien, Chi-Hsiang; Chen, Chih-Yen; Yuh, Chiou‐Hwa; Chen, Chun‐Ju; Lin, Yung‐Sheng

doi:10.1016/j.measurement.2018.06.033

Cited by 5 publications

(7 citation statements)

References 29 publications

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“…These steps were all conducted in a light-shielded environment. A compact fluorescence detection system comprising a fiber-coupled light-emitting diode and photodiode sensor 2 was used to measure the photobleaching and recovery dynamics of these samples at a constant temperature (25 °C). The 10 minutes irradiation of 470 nm LED was applied for photobleaching and then recovery observation was followed at predetermined time points until reaching plateau of fluorescence intensity.…”

Section: Methodsmentioning

confidence: 99%

“…Fluorescence technology plays a pivotal role in the field of biomedical research. 1,2 A common challenge in using this technology is photobleaching, which affects the effectiveness of fluorescence imaging in clinical medicine. Understanding photobleaching kinetics is essential for effectively addressing its occurrence.…”

Section: Introductionmentioning

confidence: 99%

“…However, the use of FRAP for conducting long-term photobleaching experiments to investigate molecular dynamics is relatively uncommon. Our previous study involved conducting extended photobleaching experiments using a self-constructed laser-induced fluorescence system, 2 but we did not explore the subsequent recovery kinetics.…”

Section: Introductionmentioning

confidence: 99%

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Fluorescence photobleaching and recovery of fluorescein sodium in carbomer film

Lin,

Chen,

Yang

2024

RSC Adv.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fluorescence photobleaching and recovery of fluorescein sodium in carbomer film

Lin,

Chen,

Yang

2024

RSC Adv.

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“…28 In addition, fluorescent proteins are always blended with polymers to probe specific functionalities, especially as a model of drug release. 29 The performance of fluorescent molecules is closely related to both intrinsic properties (selfquenching 30 ) and the chemical environment in which they are located. pH, 31 grafting mode, 32 and solvents 33 are major effecting factors of fluorescence.…”

Section: Preparation Degradable Fluorescence-grafted Copolymers (Pu-5)mentioning

confidence: 99%

Biodegradable Polyurethane Scaffolds in Regeneration Therapy: Characterization and In Vivo Real-Time Degradation Monitoring by Grafted Fluorescent Tracer

Lei,

Yang,

Chen

et al. 2023

ACS Appl. Mater. Interfaces

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There is an urgent need to assess material degradation in situ and in real time for their promising application in regeneration therapy. However, traditional monitoring methods in vitro cannot always profile the complicated behavior in vivo. This study designed and synthesized a new biodegradable polyurethane (PU-P) scaffold with polycaprolactone glycol, isophorone diisocyanate, and L-lysine ethyl ester dihydrochloride. To monitor the degradation process of PU-P, calcein was introduced into the backbone (PU-5) as a chromophore tracing in different sites of the body and undegradable fluorescent scaffold (CPU-5) as the control group. Both PU-P and PU-5 can be enzymatically degraded, and the degradation products are molecularly small and biosafe. Meanwhile, by virtue of calcein anchoring with urethane, polymer chains of PU-5 have maintained the conformational stability and extended the system conjugation, raising a structure-induced emission effect that successfully achieved a significant enhancement in the fluorescence intensity better than pristine calcein. Evidently, unlike the weak fluorescent response of CPU-5, PU-5 and its degradation can be clearly imaged and monitored in real time after implantation in the subcutaneous tissue of nude mice. Meanwhile, the in situ osteogeneration has also been promoted after the two degradable scaffolds have been implanted in the rabbit femoral condyles and degraded with time. To sum up, the strategy of underpinning tracers into degradable polymer chains provides a possible and effective way for real-time monitoring of the degradation process of implants in vivo.

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“…In addition, a SYBR green reagent-based PCR was conducted in the droplet microfluidic PCR chip. The fluorescence intensity of the PCR product of peanut template DNA was determined using a previously developed compact fluorescent system with a fiber-coupled light-emitting diode (LED) [24]. In this detection system, a 12 μL sample solution was excited using a 470 nm LED, and fluorescence with a wavelength between 505 and 545 nm was detected.…”

Section: Evaporation Of Water In the Microfluidicmentioning

confidence: 99%

Peanut Detection Using Droplet Microfluidic Polymerase Chain Reaction Device

Chiang

Hsu

et al. 2019

Journal of Sensors

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In this study, we integrated genetic detection for polymerase chain reaction (PCR) with microfluidics technology for the detection of peanut DNA. A cross-junction microchannel was used to induce emulsion droplets of water in oil for PCR on a chip. Compared with the single-phase flow, the emulsion droplet flow exhibited a 7.24% lower evaporation amount and prevented air bubble generation. PCR results of the droplet microfluidic PCR chip for peanut DNA fragment detection was verified by comparison with a commercial PCR thermal cycler and increased fluorescence intensity in SYBR Green reagent-based PCR. Moreover, PCR on the microfluidic PCR chip was successful for sesame, Salmonella spp., and Staphylococcus aureus. The droplet microfluidic PCR device developed in this study can be applied for peanut detection in the context of food allergy.

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Compact fluorescence system with fiber-coupled LED for studying photobleaching

Cited by 5 publications

References 29 publications

Fluorescence photobleaching and recovery of fluorescein sodium in carbomer film

Fluorescence photobleaching and recovery of fluorescein sodium in carbomer film

Biodegradable Polyurethane Scaffolds in Regeneration Therapy: Characterization and In Vivo Real-Time Degradation Monitoring by Grafted Fluorescent Tracer

Peanut Detection Using Droplet Microfluidic Polymerase Chain Reaction Device

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