New general results of non-existence and rigidity of spacelike submanifolds immersed in a spacetime, whose mean curvature is a timeoriented causal vector field, are given. These results hold for a wide class of spacetimes which includes globally hyperbolic, stationary, conformally stationary and pp-wave spacetimes, among others. Moreover, applications to the Cauchy problem in General Relativity, are presented. Finally, in the case of hypersurfaces, we also obtain significant consequences in Geometrical Analysis, solving new Calabi-Bernstein and Dirichlet problems on a Riemannian manifold.