Compact plasmonic optical biosensors based on nanostructured gradient index lenses integrated into microfluidic cells

Horrer, Andreas; Haas, Jonas; Freudenberger, Kathrin; Gauglitz, Günter; Kern, D. P.; Fleischer, Monika

doi:10.1039/c7nr04097k

Cited by 15 publications

(12 citation statements)

References 34 publications

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“…Horrer et al, report about the plasmonic optical biosensor integrated into a micro-fluidic cell. Also, in this work, the optical structure is made of glass and the microfluidic channel is realized in PDMS [ 62 ]. Alsabbagh et al, reported an impedance-based biosensor realized on a glass substrate bonded with a PDMS microfluidic channel [ 63 ].…”

Section: Methods For Manufacturing Microfluidic Structures For Biosen...mentioning

confidence: 99%

Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures

et al. 2022

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Proteins in biological fluids (blood, urine, cerebrospinal fluid) are important biomarkers of various pathological conditions. Protein biomarkers detection and quantification have been proven to be an indispensable diagnostic tool in clinical practice. There is a growing tendency towards using portable diagnostic biosensor devices for point-of-care (POC) analysis based on microfluidic technology as an alternative to conventional laboratory protein assays. In contrast to universally accepted analytical methods involving protein labeling, label-free approaches often allow the development of biosensors with minimal requirements for sample preparation by omitting expensive labelling reagents. The aim of the present work is to review the variety of physical label-free techniques of protein detection and characterization which are suitable for application in micro-fluidic structures and analyze the technological and material aspects of label-free biosensors that implement these methods. The most widely used optical and impedance spectroscopy techniques: absorption, fluorescence, surface plasmon resonance, Raman scattering, and interferometry, as well as new trends in photonics are reviewed. The challenges of materials selection, surfaces tailoring in microfluidic structures, and enhancement of the sensitivity and miniaturization of biosensor systems are discussed. The review provides an overview for current advances and future trends in microfluidics integrated technologies for label-free protein biomarkers detection and discusses existing challenges and a way towards novel solutions.

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Section: Methods For Manufacturing Microfluidic Structures For Biosen...mentioning

confidence: 99%

Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures

et al. 2022

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“…Furthermore, it offers a better reproducibility of the analysis by controlling the LSPR of the interaction of the nanostructured array. In addition, such kinds of anisotropic patterned nanoparticle array-based biosensors can integrate with microfluidic technology for multiplexed and ultrasensitive analysis [ 86 , 87 , 88 , 89 , 90 ].…”

Section: Plasmonic Biosensors Based On Patterned Metallic Nanostructure Arraysmentioning

confidence: 99%

Microfluidics-Based Plasmonic Biosensing System Based on Patterned Plasmonic Nanostructure Arrays

Liu

Zhang

2021

Micromachines

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This review aims to summarize the recent advances and progress of plasmonic biosensors based on patterned plasmonic nanostructure arrays that are integrated with microfluidic chips for various biomedical detection applications. The plasmonic biosensors have made rapid progress in miniaturization sensors with greatly enhanced performance through the continuous advances in plasmon resonance techniques such as surface plasmon resonance (SPR) and localized SPR (LSPR)-based refractive index sensing, SPR imaging (SPRi), and surface-enhanced Raman scattering (SERS). Meanwhile, microfluidic integration promotes multiplexing opportunities for the plasmonic biosensors in the simultaneous detection of multiple analytes. Particularly, different types of microfluidic-integrated plasmonic biosensor systems based on versatile patterned plasmonic nanostructured arrays were reviewed comprehensively, including their methods and relevant typical works. The microfluidics-based plasmonic biosensors provide a high-throughput platform for the biochemical molecular analysis with the advantages such as ultra-high sensitivity, label-free, and real time performance; thus, they continue to benefit the existing and emerging applications of biomedical studies, chemical analyses, and point-of-care diagnostics.

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“…GRIN lenses achieve their focusing properties by spatially varying internal refractive index and image directly the metallic nanostructures as an objective being automatically in focus [19]. Compared with standard microscope objectives, this configuration is more compact and offers advantages in such imaging setups [175]. For imaging systems, the measurement of more than 100 spots in parallel is expected.…”

Section: Fibers and Waveguides Without Structurementioning

confidence: 99%

Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects

Gauglitz

2020

Anal Bioanal Chem

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Direct optical detection has proven to be a highly interesting tool in biomolecular interaction analysis to be used in drug discovery, ligand/receptor interactions, environmental analysis, clinical diagnostics, screening of large data volumes in immunology, cancer therapy, or personalized medicine. In this review, the fundamental optical principles and applications are reviewed. Devices are based on concepts such as refractometry, evanescent field, waveguides modes, reflectometry, resonance and/or interference. They are realized in ring resonators; prism couplers; surface plasmon resonance; resonant mirror; Bragg grating; grating couplers; photonic crystals, Mach-Zehnder, Young, Hartman interferometers; backscattering; ellipsometry; or reflectance interferometry. The physical theories of various optical principles have already been reviewed in detail elsewhere and are therefore only cited. This review provides an overall survey on the application of these methods in direct optical biosensing. The "historical" development of the main principles is given to understand the various, and sometimes only slightly modified variations published as "new" methods or the use of a new acronym and commercialization by different companies. Improvement of optics is only one way to increase the quality of biosensors. Additional essential aspects are the surface modification of transducers, immobilization strategies, selection of recognition elements, the influence of non-specific interaction, selectivity, and sensitivity. Furthermore, papers use for reporting minimal amounts of detectable analyte terms such as value of mass, moles, grams, or mol/L which are difficult to compare. Both these essential aspects (i.e., biochemistry and the presentation of LOD values) can be discussed only in brief (but references are provided) in order to prevent the paper from becoming too long. The review will concentrate on a comparison of the optical methods, their application, and the resulting bioanalytical quality.

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Compact plasmonic optical biosensors based on nanostructured gradient index lenses integrated into microfluidic cells

Cited by 15 publications

References 34 publications

Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures

Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures

Microfluidics-Based Plasmonic Biosensing System Based on Patterned Plasmonic Nanostructure Arrays

Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects

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