Compactin production in solid-state fermentation using orthogonal array method by P. brevicompactum

Shaligram, Nikhil S.; Singh, S. N.; Szakács, George; Pandey, Ashok

doi:10.1016/j.bej.2008.05.011

Cited by 27 publications

(18 citation statements)

References 23 publications

(33 reference statements)

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“…This one-dimensional evaluation is tedious and time-consuming and it usually does not lead to the determination of optimal conditions, mainly due to ignoring interactions [1]. However, a full factorial design results in a large number of experiments [21]. As a solution, fractional factorial experimental designs, including Placket-Burman design, OAM, and RSM have been introduced, which reduce the number of tests while giving reliable results [27].…”

Section: Optimization Of Salts Using Response Surface Methodsmentioning

confidence: 99%

“…Taguchi's design uses a special set of arrays called orthogonal arrays, which gives the minimal number of experiments, and provides full information on all factors that affect the performance parameter [21]. RSM employing the central composite design (CCD) is the collection of mathematical and statistical techniques, which can be used to identify the effect of individual variables, evaluate the relative significance, and expert optimal conditions [8].…”

Section: Optimization Of Salts Using Response Surface Methodsmentioning

confidence: 99%

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Comparison of Statistical Methods for Optimization of Salts in Medium for Production of Carboxymethylcellulase of Bacillus amyloliquefaciens DL-3 by a Recombinant E. coli JM109/DL-3

Lee¹,

Kim²,

Gao³

et al. 2011

Journal of Life Science

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The optimal concentrations of salts in medium for cell growth and the production of carboxymethylcellulase (CMCase) by a recombinant E. coli JM109/DL-3 were established using two statistical methods: orthogonal array method (OAM) and response surface method (RSM). The analysis of variance (ANOVA) of data based on OAM indicated that K2HPO4 gave maximum sum of square (S) and percentage contribution (P) for cell growth as well as production of CMCase. The optimal concentrations of K2HPO4, NaCl, MgSO4•7H2O, and (NH4)2SO4 in medium for cell growth extracted by Qualitek-4 (W32b) Software were 10.0, 1.0, 0.2, and 0.6 g/l, respectively, whereas those for the production of CMCase by E. coli JM109/DL-3 were 5.0, 1.0, 0.4, and 0.6 g/l. The analysis of variance (ANOVA) resulting from RSM indicated that a highly significant salt for cell growth was K2HPO4 ("probe>F" less than 0.0001), whereas K2HPO4 and MgSO4•7H2O were significant for the production of CMCase. The optimal concentrations of K2HPO4, NaCl, MgSO4•7H2O, and (NH4)2SO4 for cell growth extracted by Design Expert Software were 7.44, 1.08, 0.22, and 0.88 g/l, respectively, whereas those for production of CMCase were 5.84, 0.69, 0.28, and 0.54 g/l. The optimal concentrations of salts and their influences on cell growth and production of CMCase extracted by OAM were almost the same as those by RSM. Production of CMCase by a recombinant E. coli JM109/DL-3 under optimized concentration of salts was 1.93 times higher than that by Bacillus amyloliquifaciens DL-3.

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Section: Optimization Of Salts Using Response Surface Methodsmentioning

confidence: 99%

Section: Optimization Of Salts Using Response Surface Methodsmentioning

confidence: 99%

Comparison of Statistical Methods for Optimization of Salts in Medium for Production of Carboxymethylcellulase of Bacillus amyloliquefaciens DL-3 by a Recombinant E. coli JM109/DL-3

Lee¹,

Kim²,

Gao³

et al. 2011

Journal of Life Science

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“…Among the few microbial strains for mevastatin production are Penicillium citrinum [26], Penicillium cyclopium [28], and Penicillium brevicompactum [29]. Although mevastatin is not used directly in treatment, it is an important intermediate compound in industrial pravastatin production [7].…”

Section: Statin-producing Ability Of Fungal Isolates 32mentioning

confidence: 99%

Novel Pravastatin-Producing Penicillium janthinellum Strain Isolated from Soil

Seydametova¹

2015

IJBBB

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Hyperlipidemia is a main risk factor for coronary heart disease and stroke. The lipid-lowering drug, pravastatin, is currently obtained by the microbial biotransformation of compactin (mevastatin) to pravastatin. It is clear that pharmaceutical industry has to date utilized only a very minor portion of nature's microbial arsenal for the discovery of pravastatin. Therefore, the search for microfungi which is able to produce this statin directly has gained increasing importance. In this study, a total of 33 soil fungal cultures were isolated from nine sampling sites in oil palm plantations in Malaysia and tested for their potential to produce pravastatin. The cultures were cultivated in submerged fermentation followed by screening for statin production using high-performance liquid chromatography. The best pravastatin producer was identified to species level using morphological characteristics and molecular biological approaches. From the tested cultures, 18 fungal isolates were able to produce pravastatin directly by fermentation. Among these, the isolate ESF20P was the best producer, with a yield of 15.8 mg/l pravastatin. Molecular identification of this strain showed the highest homology (98%) with Penicillium janthinellum.

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“…[8] Production of secondary metabolites, has been found to be independent from growth. Shaligram et al, (2008) [6] reported highest yield of mevastatin with maltose as an additional carbon source by using Pencillium brevicompactum from wheat bran and groundnut oil cake under SSF. [6] reported highest yield of mevastatin with ammonium nitrate by using pencillium brevicompactum from wheat bran and groundnut oil cake under SSF.…”

Section: Effect Of Additional Carbon Supplementation On Mevastatin Prmentioning

confidence: 99%

“…To determine the optimum age of inoculum for mevastatin production, 5 g of substrate autoclaved and moistened with 60 % v/w moisturizing medium pH 6.0, 3 ml inoculum was added at [6] reported highest yield of mevastatin with 12 day old inoculum of Pencillium brevicompactum from wheat bran and groundnut oil cake under SSF. …”

Section: Effect Of Age Of Inoculummentioning

confidence: 99%

Optimization of Physico Chemical and Nutritional Parameters for the Production of Mevastatin Using Pencillium citrinum MTCC 1256

Rao¹

2013

iosrphr

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Abstract:-Statins are the inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, are the class of drugs which are used in the treatment of hypercholesterolemia. They are the most efficient drugs for reducing plasma cholesterol. Mevastatin, also known as compactin is also a statin, catalyzes the conversion of HMG-CoA to mevanolate. Mevanolate is a required building block for cholesterol biosynthesis and mevastatin interferes with its production by acting as a reversible competitive inhibitor for HMG-CoA reductase and thus blocking the enzyme. The present study was carried out on the production of mevastatin by solid state fermentation with Pencillium citrinum MTCC 1256 using sesame oil cake at 28 o C. It deals with the study of parameters such as inoculum age, inoculum volume; nutritional suppliments such as carbon source, nitrogen source and their concentrations were optimized for the effective production of mevastatin.

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Compactin production in solid-state fermentation using orthogonal array method by P. brevicompactum

Cited by 27 publications

References 23 publications

Comparison of Statistical Methods for Optimization of Salts in Medium for Production of Carboxymethylcellulase of Bacillus amyloliquefaciens DL-3 by a Recombinant E. coli JM109/DL-3

Comparison of Statistical Methods for Optimization of Salts in Medium for Production of Carboxymethylcellulase of Bacillus amyloliquefaciens DL-3 by a Recombinant E. coli JM109/DL-3

Novel Pravastatin-Producing Penicillium janthinellum Strain Isolated from Soil

Optimization of Physico Chemical and Nutritional Parameters for the Production of Mevastatin Using Pencillium citrinum MTCC 1256

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