Comparable bone healing capacity of different bone graft matrices in a rabbit segmental defect model

Abstract

We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model. Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation. DBX showed significantly lower radiopacity, bone volume fraction, and bone mineral density than DB and NDDB before implantation. However, bone healing score, bone volume fraction, bone mineral density, and residual bone area at 4, 8, and 12 weeks post-implantation revealed no significant differences in bone healing capacity. Overall, three DBM products with different carrier molecules or bone compositions showed similar bone healing capacity.

Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration

“…Osteoprogenitor cell recruitment along with the formation of new bone surrounding DBM particles were noted within 4 weeks after implantation. Effective BMSC recruitment was also evidenced by the presence of newly formed trabeculae within 8 weeks and full repair of mature bone by 12 weeks [168]. Clinical pilot studies have deemed DBM equivalent to iliac crest ABG for the treatment of non-unions or void spaces due to the removal of benign tumors [169] as well as the treatment of long bone fractures [170].…”

Repair of surgical bone defects grafted with hydroxylapatite + β-TCP combined with hyaluronic acid and collagen membrane in rabbits: A histological study

“…These results correlated with those of Kim et al., which demonstrated the importance of osteoconductive and osteoinduction processes. Hyaluronic acid and grafting significantly enhanced the healing process when used both alone or together due to increased morphogenesis and tissue healing during bone regeneration 13 . When hyaluronic acid comes into contact with bone it participates in bone morphogenesis and plays an important part in the early events of the osteogenic process, 24 modifying the effects of several growth factors and cytokines.…”

“…The heath of the rabbits was continuously monitored during the experiment by a veterinarian. The animals were randomly distributed into three groups (5 rabbits/group) according to the period before animal sacrifice (1, 2, or 4 weeks) 13 . For each animal, four bone defects were made as follows (Table 1): (i) Defect I (D1, control) in right tibia.…”

Injectable macromolecule-based calcium phosphate bone substitutes

“…56 Alternative formulations have been developed, such as by either switching the inorganic filler with another natural version of synthetic calcium phosphate (e.g. ), demineralized bone matrix [57][58][59] or by changing the nature of the viscous carrier (e.g., hyaluronic acid, 60 gelatin, 61 or collagen 62 ). Comparable overall bone regeneration was observed.…”