Comparação de métodos para a detecção de Fusarium graminearum em sementes de trigo (Triticum aestivum L.)

Júnior, Daniel Garcia; Vechiato, M. H.; Menten, José Otávio Machado

doi:10.1590/s0100-54052008000200010

Cited by 4 publications

(4 citation statements)

References 2 publications

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“…Therefore, BTF presented a lower sensibility to detect F. graminearum compared to PDA medium. These results differed from that obtained by Garcia Júnior et al (2008), in which no significant differences were observed on F. graminearum incidence between semi-selective mediums and BTF. The results obtained in the study showed that is not always possible to safely detect all fungi in wheat seeds by the same method.…”

Section: Resultscontrasting

confidence: 99%

“…Through seeds, microorganisms can be transported for long distances and easily introduced into new areas (Nasser, 1987). The incidence of F. graminearum on seeds was quantified in many studies (Telles Neto et al, 2007;Kabeere et al, 1997;Garcia Júnior et al, 2008;Kobayasti and Pires, 2011) and could be up to 48% (Kabeere et al, 1997;Danelli et al, 2012). For P. grisea, the pathogen incidence was also measured in different studies, and most of them reported incidences about 6 to 18% (Goulart and Paiva, 1991;Goulart and Paiva, 1993).…”

Section: Introductionmentioning

confidence: 99%

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Efficiency of Blotter test and agar culture medium to detect Fusarium graminearum and Pyricularia grisea in wheat seeds

Camargo¹,

Moraes²,

Menten³

2017

J. Seed Sci.

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-Seeds can be considered one of the most efficient forms to disseminate pathogens. Therefore, the use of healthy seeds is extremely important to establish a crop, and seed health testing must be performed to determine the seed sanitary quality. This study aimed to compare the efficiency of seed health testing to detect Pyricularia grisea and Fusarium graminearum in three samples of wheat seeds. Methods evaluated were Blotter test with freezing (BTF) and potato-dextrose-agar culture medium (PDA). The incubation temperatures were 20 ºC and 25 ºC. From each sample, a subsample was submitted to seed asepsis using hypochlorite solution (1%) and another analyzed without seed asepsis. The temperatures evaluated did not influence the detection of the pathogens. P. grisea incidence ranged from 4.5 to 17% with BTF without seed asepsis. The BTF with seed asepsis and PDA (with and without seed asepsis) presented pathogen incidence no higher than 1.5%. Moreover, results suggested that most part of P. grisea inoculum was presented externally on seed tissues. PDA medium was more efficient to detect F. graminearum, independently of seed asepsis. By this method, pathogen incidence ranged from 3 to 39%. F. graminearum incidence using BTF with seed asepsis varied from 0.5 to 1.5% and BTF without seed asepsis presented a pathogen incidence of 5.0 to 12.5%. The Blotter test with freezing was more efficient to detect P. grisea while the PDA medium was more efficient to detect F. graminearum.Index terms: Triticum aestivum, detection methods, seed pathology.Eficiência do método do papel de filtro e do meio de cultura agarizado na detecção de Fusarium graminearum e Pyricularia grisea em sementes de trigo RESUMO -Sementes podem ser consideradas como uma das formas mais eficientes na disseminação de patógenos. Portanto, o uso de sementes sadias é de extrema importância para o estabelecimento de uma cultura e testes de sanidade devem ser realizados para determinar a sua qualidade sanitária. O presente trabalho teve como objetivo comparar a eficiência de métodos de sanidade na detecção de Pyricularia grisea e Fusarium graminearum em três amostras de sementes de trigo. Os métodos avaliados foram o papel de filtro com congelamento (PFC) e o meio de cultura batata-dextrose-ágar (BDA). As temperaturas de incubação foram 20 ºC e 25 ºC. De cada amostra, uma submostra foi submetida a assepsia de sementes usando solução de hipoclorito (1%) e outra analisada sem assepsia. As temperaturas analisadas não influenciaram na detecção dos patógenos. A incidência de P. grisea variou de 4,5 a 17% no método PFC sem assepsia das sementes. O PFC com assepsia e o BDA (com e sem assepsia) apresentou incidência do patógeno inferior a 1,5%. Além disso, os resultados sugeriram que a maior parte do inóculo de P. grisea estava presente externamente aos tecidos da semente. O meio BDA foi mais eficiente na detecção de F. graminearum, independentemente da assepsia das sementes. Por esse método, a incidência do patógeno variou de 3 a 39%. A incidência de F. grami...

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Section: Resultscontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficiency of Blotter test and agar culture medium to detect Fusarium graminearum and Pyricularia grisea in wheat seeds

Camargo¹,

Moraes²,

Menten³

2017

J. Seed Sci.

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“…2008. Além disto, Garcia Júnior et al (2008) relataram que a detecção de F. graminearum pode ser prejudicada pela alta incidência de fungos saprofíticos, como Cladosporium sp.…”

Section: Resultsunclassified

Levantamento De Fungos Em Sementes De Trigo

Kobayasti

Pires

2011

Pesq. Agropec. Trop.

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está concentrada no Sul do Brasil. O cereal vem sendo introduzido paulatinamente na região do Cerrado, sob irrigação ou sequeiro (Embrapa 2007). Desde dezembro de 2003, o Estado de Mato Grosso está oficialmente incluso no zoneamento agrícola do Ministério da Agricultura, Pecuária e Abastecimento (MAPA), por meio da Portaria 131, a qual estabelece o plantio de trigo sequeiro entre 1º e 29 de fevereiro, em regiões com altitude igual ou superior a 750 m, e o plantio irrigado entre 21 de abril e 20 de maio, em municípios com altitude superior a 600 m (Brasil 2003).

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“…Water stress is used to control or inhibit seed germination under different circumstances such as in health tests on paper substrate (CELANO et al, 2012) and potato dextrose agar medium (PDA) (GARCIA JÚNIOR et al, 2008), and in fungal inoculation tests (FARIAS et al, 2010), besides simulating water deficit (GIROTTO et al, 2012), osmotic and matric conditioning (QUEIROGA et al, 2011) for crops of interest. A water restriction condition can be obtained by adjusting water potential with osmotically active solutes, with the most common being mannitol, polyethylene glycol, maltose (GIROTTO et al, 2012), KCl, NaCl, sucrose (GARCIA JÚNIOR et al, 2008), or CaCl 2 (MACHADO NETO et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Water potential and time of Pyrenophora tritici-repentis inoculation in wheat seeds

Bertagnolli

Deuner

Frizon

et al. 2017

SCA

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Wheat seeds infected with Pyrenophora tritici-repentis, the causal agent of tan spot, are partially responsible for outbreaks caused by this pathogen. Seed lots with a high incidence of P. tritici-repentis must be rapidly acquired for transmission and pathogen-control studies. Therefore, the aim of this study was to determine whether changes in water potential of culture medium and variations in inoculation time might favor the infection of wheat seeds by P. tritici-repentis without compromising seed viability. Colonies of P. tritici-repentis were grown on potato-dextrose-agar (PDA) culture medium, adjusted to a water potential of -0.36 MPa, under water stress induced by mannitol at potentials of -0.4, -0.6, -0.8, -1.0, and -1.2 MPa. Analyses were carried out to determine mycelial growth index and seed exposure time to the culture medium to start germination as a function of water potential. Afterwards, wheat seeds were placed in contact with colonies of P. tritici-repentis for 24, 48, and 72 hours at potentials of -0.4, -0.6, -0.8, -1.0, and -1.2 MPa. The experiment was arranged in a completely randomized design, in a factorial scheme (water potential × inoculation time). Rates of germination, seedling emergence in soil, and seed infection were assessed. Mycelial growth was stimulated at lower water potentials, which germinated faster. A 24-hour inoculation time and a -0.4 MPa water potential were efficient to infect wheat seeds with P. tritici-repentis, without hindering seedling germination and emergence under laboratory conditions.

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Comparação de métodos para a detecção de Fusarium graminearum em sementes de trigo (Triticum aestivum L.)

Cited by 4 publications

References 2 publications

Efficiency of Blotter test and agar culture medium to detect Fusarium graminearum and Pyricularia grisea in wheat seeds

Efficiency of Blotter test and agar culture medium to detect Fusarium graminearum and Pyricularia grisea in wheat seeds

Levantamento De Fungos Em Sementes De Trigo

Water potential and time of Pyrenophora tritici-repentis inoculation in wheat seeds

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