Comparative analysis between aerial parts and roots (<i>Astragali Radix</i>) of <i>astragalus membranaceus</i> by NMR-based metabolomics

Wang, Erbing; Jin, Bao-Fen; Li, Xia; Liu, Ruiling; Xie, Xinran; Guo, Weifeng; Zheng, Huixia; Zheng-bao, Zhao

doi:10.1080/09540105.2017.1332007

Cited by 10 publications

(6 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, a new UPLC-Qtrap HRMS method was developed to identify the secondary metabolites in APAM and AR, and a 1 H NMR-based metabolomics was used to examine the pharmacological effects of APAR and AR in N-acetyl phenylhydrazine (APH) and Cy-treated mice. In combination with our previous study (Wang, Jin, et al, 2017), the results of this study are expected to contribute significantly to the establishment of a scientific basis for the comprehensive utilization of APAM.…”

Section: Introductionsupporting

confidence: 56%

“…Glutamate is an intermediate product of glutamine metabolism (Wang, Jin, et al, 2017) and may affect the immune response of monocytes/macrophages, the proliferation of lymphocytes, and the synthesis of Hsp70 (Exner et al, 2003). Glutathione (GSH), a tripeptide of glutamate, glycine, and cysteine, is an efficient antioxidant that provides protection against oxidative stress, either by serving as a cofactor for some antioxidant enzymes or by reducing reactive oxygen species (ROS) (Lu, 2013;Roth et al, 2002;Yuan & Kaplowitzc, 2009).…”

Section: D-glutamine and D-glutamate Metabolismmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of aerial parts of Astragalus membranaceus and Astragali Radix based on chemical constituents and pharmacological effects

Wang

Lü

et al. 2019

Food and Agricultural Immunology

Self Cite

View full text Add to dashboard Cite

This study aims to provide a scientific basis for the comprehensive utilization of the aerial parts of Astragalus membranaceus (APAM). UPLC-Qtrap HRMS was used to identify secondary metabolites in APAM and Astragali Radix (AR), the pharmacological effects of them in N-acetyl phenylhydrazine (APH) and cyclophosphamide (Cy)-induced mice were studied by using 1 H NMR-based metabolomics. The results showed that the secondary metabolites of APAM and AR were different, but that there was no difference in the contents of eight constituent flavonoids and four constituent saponins, which were 0.8800 and 4.4351 mg/g in APAM and 0.8944 and 4.6422 mg/g in AR, respectively. APAM and AR induced beneficial effects on the recovery of body weight, blood parameters, viscera indices, Na + -K + -ATPase, and Ca 2+ -Mg 2 + -ATPase, the blood-enriching mechanisms of them were probably related to valine, leucine, and isoleucine biosynthesis, D-glutamine and D-glutamate metabolism, alanine, aspartate, and glutamate metabolism, and taurine and hypotaurine metabolism. ARTICLE HISTORY

show abstract

Section: Introductionsupporting

confidence: 56%

Section: D-glutamine and D-glutamate Metabolismmentioning

confidence: 99%

Comparison of aerial parts of Astragalus membranaceus and Astragali Radix based on chemical constituents and pharmacological effects

Wang

Lü

et al. 2019

Food and Agricultural Immunology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Taking the distribution of flavonoids and saponins in different parts of Astragalus membranaceus plant as an example, previous reports showed that the AMSL was mainly comprised of flavonoids like isoflavones and flavones, while the root as the medicinal part contained the most of saponins, followed by flavones (Li et al, 2019; Shi, Bi, & Shi, 2018). Although the AMSL and the root of Astragalus membranaceus are different from each other in primary and secondary metabolites, they gave a similar performance in terms of immune functions (Wang et al, 2017) and the recovery of body weight, blood parameters, and viscera indices in Kunming mice (Wang, Liu, et al, 2019; Wang, Xu, et al, 2019), which implies that the AMSL is an excellent resource worthy of comprehensive utilization. So far, the studies on the potential application of AMSL are mainly focused on the extensive form like the livestock and poultry breeding (Abdallah, Zhang, Elemba, Zhong, & Sun, 2020; Xi et al, 2014), and the research on the intensive development and utilization of AMSL is scarcely reported.…”

Section: Introductionmentioning

confidence: 99%

Chemical composition and antibacterial activity of ethyl acetate extract of Astragalus membranaceus aerial parts

Guo

Sun

Ping

et al. 2021

Journal of Food Safety

View full text Add to dashboard Cite

There is a growing consensus that plant‐derived antimicrobials may be a safe and effective alternative to synthetic chemical preservatives against foodborne pathogens. This study aims to investigate the potential application of the stems and leaves of Astragalus membranaceus (AMSL) in food preservation. Antibacterial activity of AMSL was evaluated by the disk diffusion method and the minimum inhibitory concentration (MIC) assay, and then the activity stability at different conditions (temperatures, pHs and ultraviolet irradiations) was analyzed and the antibacterial mechanism was revealed. Chemical composition of the active substances was also identified by UPLC–MS/MS. The results showed that the extracts from AMSL had low activities against Geotrichum candidum and Escherichia coli, while good action on Staphylococcus aureus, Bacillus subtilis, Aspergillus flavus, and Candida albicans. In contrast, the ethyl acetate fraction (EAF) of AMSL was the most active against Bacillus subtilis with a diameter of inhibition zone of 15.75 mm and the MIC value of 12.5 mg/mL. EAF exhibited good stability against heating at 80°C for 30 min, 60 min exposure to UV light, and it was effective in pH range of 2.0–6.0. After treating by EAF, the growth of the Bacillus subtilis was inhibited with delayed logarithmic phase, and the cell wall and the cell membrane of the bacteria were damaged deduced from the increased extracellular activity of alkaline phosphatase, the leakage of nucleic acids, and the increase of extracellular conductivity. Further SEM and TEM analysis confirmed the destroying of the cell wall integrity and cell membrane permeability with visually wrinkled even empty cells caused by EAF. Subsequent analysis revealed that flavonoids were the predominant components of EAF, followed by organic acids. Our findings provided a theoretical possibility that the AMSL could be used as a natural food preservative in the food industry.

show abstract

“…22,24,25 Currently, plant metabolomics has explored different states of AR. [26][27][28][29] Further, Yu et al 14 used a logistic regression algorithm to predict the quality class of AR, but the algorithm had difficulty adapting to the distribution of real nonlinear data and the quantitative data of only five differential components identified were not sufficiently representative of AR. In contrast, extreme learning machine (ELM) aims to train a single hidden layer feedforward neural network; it is faster than traditional learning algorithms with guaranteed learning accuracy.…”

Section: Introductionmentioning

confidence: 99%

A comprehensive strategy for prediction and quality evaluation of standardized planting herbs based on plant metabolomics coupled with extreme learning machine: Astragali Radix as an example

Wei

et al. 2023

Phytochemical Analysis

View full text Add to dashboard Cite

Introduction Standardizing the planting process is an effective way to control the quality stability of herbal resources, which are susceptible to external environmental factors (e.g., moisture, soil, etc.). However, how to scientifically and comprehensively assess the effects of standardized planting on plant quality and quickly test unknown samples has not been addressed. Objective The aim of this study was to determine and compare the metabolite levels of herbs before and after standardized planting, to quickly distinguish their sources, and to evaluate their quality, using the typical herb Astragali Radix (AR) as an example. Methods In this study, an efficient strategy using liquid chromatography–mass spectrometry (LC‐MS) based on plant metabolomics combined with extreme learning machine (ELM) has been developed to efficiently distinguish and predict AR after standardized planting. Moreover, a comprehensive multi‐index scoring method has been developed for the comprehensive evaluation of the quality of AR. Results The results confirmed that AR after standardized planting was significantly differentiated, with a relatively stable content of 43 differential metabolites, mainly including flavonoids. An ELM model was established based on LC‐MS data, and the accuracy in predicting unknown samples could reach more than 90%. As expected, higher total scores were obtained for AR after standardized planting, indicating much better quality. Conclusion A dual system for evaluating the impact of standardized planting on the quality of plant resources has been established, which will significantly contribute to innovation in the quality evaluation of medicinal herbs and support the selection of optimal planting conditions.

show abstract

Comparative analysis between aerial parts and roots (Astragali Radix) of astragalus membranaceus by NMR-based metabolomics

Cited by 10 publications

References 35 publications

Comparison of aerial parts of Astragalus membranaceus and Astragali Radix based on chemical constituents and pharmacological effects

Comparison of aerial parts of Astragalus membranaceus and Astragali Radix based on chemical constituents and pharmacological effects

Chemical composition and antibacterial activity of ethyl acetate extract of Astragalus membranaceus aerial parts

A comprehensive strategy for prediction and quality evaluation of standardized planting herbs based on plant metabolomics coupled with extreme learning machine: Astragali Radix as an example

Contact Info

Product

Resources

About