Comparative Analysis of a Liquid-Based Pap Test and Concurrent HPV DNA Assay of Residual Samples

Hong, In Soon; Marshalleck, Josephine J.; Williams, Robert H.; Gaiter, Thomas E.; Mielzynska‐Lohnas, Iwona; Kim, Kyung-Sook

doi:10.1159/000327055

Cited by 19 publications

(11 citation statements)

References 13 publications

(18 reference statements)

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“…Therefore, the prevalence of high-risk HPV in women with ASC in our study is comparable to that reported in the literature but leaning toward the low end of the range. [6][7][8][9] The pathologists in our laboratory who had the highest and lowest ASC:SIL ratios did not differ much in the proportion of high-risk HPV DNA positive ASC, 31% versus 32%. Furthermore, the CV of the rate of high-risk HPV DNA positive ASC was smaller than that of the ASC:SIL ratio, implying that the high-risk HPV DNA detection rate varied slightly when compared to the ASC:SIL ratio in our laboratory.…”

Section: Discussionmentioning

confidence: 99%

“…5 Various studies have reported that the HPV DNA detection rates in specimens with an ASC interpretation range from 31% to 66%. [6][7][8][9] Because of the high correlation of positive high-risk HPV results with the presence of SIL and the objective nature of HPV DNA testing, some authors have suggested the use of HPV DNA testing in quality assurance monitoring. 10,11 In the current study, we attempted to determine the relationship between the ASC:SIL ratio and the high-risk HPV DNA detection rate in ASC specimens as well as to evaluate the feasibility of using the HPV detection rate as a quality indicator.…”

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confidence: 99%

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High-Risk HPV DNA Detection Rate in Patients with Atypical Squamous Cells and Its Relationship to the Atypical Squamous Cell:Squamous Intraepithelial Lesion Ratio

Chhieng¹,

Connolly²,

Roberson³

et al. 2006

Acta Cytologica

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

High-Risk HPV DNA Detection Rate in Patients with Atypical Squamous Cells and Its Relationship to the Atypical Squamous Cell:Squamous Intraepithelial Lesion Ratio

Chhieng¹,

Connolly²,

Roberson³

et al. 2006

Acta Cytologica

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“…34 With the introduction of new techniques in screening, several studies have focused upon the sensitivity and specificity of screening techniques (table 4). [35][36][37][38][39] Although the methods of comparison and definition of endpoints may diverge in these studies, they give a comprehensive insight into what is known about the efficiency of these screening techniques at this time. When the data from our study are compared with those in the literature, Ki-67 immunostaining yields good results, especially with regard to the diagnosis of HSIL.…”

Section: Discussionmentioning

confidence: 99%

Ki-67 immunocytochemistry in liquid based cervical cytology: useful as an adjunctive tool?

Sahebali

Depuydt²,

Segers³

et al. 2003

Journal of Clinical Pathology

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Aims: To test the ability of Ki-67 to detect cytological lesions in a screening setting and its use as a surrogate marker of human papillomavirus (HPV) infection. Methods: A study of liquid based cytology, HPV DNA testing by MY09/MY11 consensus polymerase chain reaction (PCR), type specific PCRs, and Ki-67 immunocytochemistry on a randomly selected series of 147 patients. Results: Comparison of the number of Ki-67 immunoreactive cells/1000 cells in the different cytological groups showed that the HSIL group yielded a significantly higher mean count than did the other groups. The number of Ki-67 immunoreactive cells/1000 cells was significantly higher in HPV-16 positive samples than in samples containing infections with other high risk types. Receiver operating characteristic curves indicated a test accuracy (area under curve) of 0.68, 0.72, and 0.86 for atypical squamous cells of undetermined significance (ASCUS), low grade squamous intraepithelial lesions (LSIL), and high grade squamous intraepithelial lesions (HSIL), respectively. Thresholds for 95% sensitivity were 0.07, 0.08, and 0.15 Ki-67 immunopositive cells/1000 cells for ASCUS, LSIL and HSIL, respectively. The threshold for 95% specificity was 1.9 Ki-67 immunopositive cells/1000 cells. Conclusions: Ki-67 immunocytochemistry can be applied to liquid based cytology. The accuracy and diagnostic indices of the test are good when compared with those of other techniques. As part of a panel of screening procedures, it could be used as an adjunct to liquid based cytology to identify HSIL, and as a surrogate marker of HPV-16 infection.T he causal relation between genital human papillomavirus (HPV) infection and cervical dysplasia/carcinoma is well established.1-3 HPV type 16 (HPV-16) is most frequently found in cervical squamous cell carcinoma, with more than 50% of these tumours harbouring this type. 4 However, even though the prevalence of HPV infection can be as high as 60%, only 10% of infected women will develop cervical dysplasia.

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“…2 The replacement of conventional Pap tests with liquid-based specimens has facilitated testing for high-risk human papillomavirus infection, which is widely accepted as a means to triage atypical cytopathology cases in more recent years. [3][4][5][6][7][8][9][10][11][12][13][14] Both proficiency testing and human papillomavirus testing potentially bring many more data points to a cytopathology quality assurance (QA) program. However, it is not known how these metrics are being used in cytopathology as a part of a laboratory's overall QA program.…”

mentioning

confidence: 99%

College of American Pathologists Gynecologic Cytopathology Quality Consensus Conference on Good Laboratory Practices in Gynecologic Cytology: Background, Rationale, and Organization

Tworek

Henry

Blond

et al. 2013

Archives of Pathology &Amp; Laboratory Medicine

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Context.-Gynecologic cytopathology is a heavily regulated field, with Clinical Laboratory Improvement Amendments of 1988 mandating the collection of many quality metrics. There is a lack of consensus regarding methods to collect, monitor, and benchmark these data and how these data should be used in a quality assurance program. Furthermore, the introduction of human papilloma virus testing and proficiency testing has provided more data to monitor.Objective.-To determine good laboratory practices in quality assurance of gynecologic cytopathology.Data Sources.-Data were collected through a written survey consisting of 98 questions submitted to 1245

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Comparative Analysis of a Liquid-Based Pap Test and Concurrent HPV DNA Assay of Residual Samples

Cited by 19 publications

References 13 publications

High-Risk HPV DNA Detection Rate in Patients with Atypical Squamous Cells and Its Relationship to the Atypical Squamous Cell:Squamous Intraepithelial Lesion Ratio

High-Risk HPV DNA Detection Rate in Patients with Atypical Squamous Cells and Its Relationship to the Atypical Squamous Cell:Squamous Intraepithelial Lesion Ratio

Ki-67 immunocytochemistry in liquid based cervical cytology: useful as an adjunctive tool?

College of American Pathologists Gynecologic Cytopathology Quality Consensus Conference on Good Laboratory Practices in Gynecologic Cytology: Background, Rationale, and Organization

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