Comparative Analysis of Antigiardial Potential of Heat Inactivated and Probiotic Protein of Probiotic Lactobacillus rhamnosus GG in Murine Giardiasis

Shukla, Geeta; Kamboj, Shweta; Sharma, Bhawna

doi:10.1007/s12602-018-9506-8

Cited by 11 publications

(4 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RL preparation. The preparation of RL was performed as described by Shukla et al (47) and Arai et al (48). L. rhamnosus ATCC 7469 was cultivated and maintained in MRS broth at 37°C for 24 h. Overnightcultivated strains were centrifuged at 1200 g for 15 min and then washed twice with NaCl.…”

Section: Methodsmentioning

confidence: 99%

Heat‐KilledLactobacillus rhamnosusATCC7469 ImprovedUVB‐InducedPhotoagingViaAntiwrinkleandAntimelanogenesisImpacts

Zhang

et al. 2023

Photochem & Photobiology

View full text Add to dashboard Cite

Exposure of ultraviolet B (UVB) radiation is the main factor from the environment to cause skin photoaging. Lactobacillus rhamnosus ATCC 7469, is a probiotic strain with a good track record for enhancing human health. The present study conducted the impacts of heat‐killed L. rhamnosus ATCC 7469 (RL) on photoaging in vitro using mouse skin fibroblast (MSF) cells and human epidermal melanocytes (HEM) exposed to UVB. The results showed that (1) RL‐protected UVB‐induced cytotoxicity relating to absorb UVB and reduce DNA damage. (2) RL exerted the antiwrinkle impact involved in two aspects. Firstly, RL downregulated MMP‐1, 2, 3 expressions associating with MAPK signaling, resulting in the increased the protein expression of COL1A1, further booting type I collagen abundant thereby promoting the antiwrinkle impact in MSF cells. Secondly, RL reduced ROS content, further decreasing oxidative damage relating to Nrf2/Sirt3/SOD2 signaling, thereby promoting the antiwrinkle impact in MSF cells. (3) RL suppressed tyrosinase and TYRP‐2 activity and/or levels associating with PKA/CREB/MITF signaling, thereby promoting antimelanogenesis impact in HEM cells. In conclusion, our findings suggest that RL could reduce photoaging caused by UVB via antiwrinkle and antimelanogenesis properties and may be a potential antiphotoaging beneficial component, which is applied in the cosmetic industry.

show abstract

Section: Methodsmentioning

confidence: 99%

Heat‐KilledLactobacillus rhamnosusATCC7469 ImprovedUVB‐InducedPhotoagingViaAntiwrinkleandAntimelanogenesisImpacts

Zhang

et al. 2023

Photochem & Photobiology

View full text Add to dashboard Cite

show abstract

“…The preparation of PL was performed as described by Shukla et al (2020) and Arai et al (2018) [ 40 , 41 ]. Lacticaseibacillus paracasei was cultivated and maintained in MRS broth at 37 °C for 24 h. Overnight-cultivated strains were centrifuged at 1200× g for 15 min, washed twice with phosphate-buffered saline (PBS), and then washed twice with sterile distilled water.…”

Section: Methodsmentioning

confidence: 99%

Heat-Killed Lacticaseibacillus paracasei Ameliorated UVB-Induced Oxidative Damage and Photoaging and Its Underlying Mechanisms

Zhang

Song

et al. 2022

Antioxidants

View full text Add to dashboard Cite

Ultraviolet B (UVB) radiation is a major environmental causative factor of skin oxidative damage and photoaging. Lacticaseibacillus paracasei is a well-known probiotic strain that can regulate skin health. The present study investigated the effects of heat-killed Lacticaseibacillus paracasei (PL) on UVB linked oxidative damage and photoaging in skin cells (Normal human dermal fibroblast (NHDF) cells and B16F10 murine melanoma cells). Results demonstrated that: (1) PL prevented UVB-induced cytotoxicity relating to decreased DNA damage in NHDF and B16F10 cells; (2) PL alleviated UVB-induced oxidative damage through increasing GSH content, as well as antioxidant enzyme activities and mRNA levels (except MnSOD activity and mRNA levels as well as CAT mRNA level) relating to the activation of Sirt1/PGC-1α/Nrf2 signaling in NHDF cells; (3) PL attenuated UVB-induced photoaging was noticed with a decrease in the percentage of SA-β-gal positive cells in NHDF cells model. Moreover, PL attenuated UVB-induced photoaging through exerting an anti-wrinkling effect by enhancing the type I collagen level relating to the inhibition (JNK, p38)/(c-Fos, c-Jun) of signaling in NHDF cells, and exerting an anti-melanogenic effect by suppressing tyrosinase and TYRP-1 activity and/or expressions relating to the inhibition of PKA/CREB/MITF signaling in B16F10 cells. In conclusion, PL could ameliorate UVB-induced oxidative damage and photoaging. Therefore, PL may be a potential antioxidant and anti-photoaging active ingredient for the cosmetic industry.

show abstract

“…Other lactobacillus strains, including L. casei MTCC 1423 and L. rhamnosus, have been tested in a murine model, showed similar protective effects, and reduced the severity/duration of giardiasis [ 92 , 93 , 94 ]. Interestingly, Shukla et al (2020) reported an anti-giardia effect and enhanced mucosal immunity by the probiotic protein from L. rhamnosus as well as the heat-killed strain [ 95 ]. Although the underlying mechanism remains poorly understood [ 96 , 97 , 98 ], recent reports demonstrate a potential association between BSH activity and the protective effect against G. duodenalis infection [ 99 , 100 ].…”

Section: Therapeutic Relevance On Bsh-active Bacteriamentioning

confidence: 99%

Bile Salt Hydrolases: At the Crossroads of Microbiota and Human Health

et al. 2021

View full text Add to dashboard Cite

The gut microbiota has been increasingly linked to metabolic health and disease over the last few decades. Several factors have been suggested to be involved in lipid metabolism and metabolic responses. One mediator that has gained great interest as a clinically important enzyme is bile salt hydrolase (BSH). BSH enzymes are widely distributed in human gastrointestinal microbial communities and are believed to play key roles in both microbial and host physiology. In this review, we discuss the current evidence related to the role of BSHs in health and provide useful insights that may pave the way for new therapeutic targets in human diseases.

show abstract

Comparative Analysis of Antigiardial Potential of Heat Inactivated and Probiotic Protein of Probiotic Lactobacillus rhamnosus GG in Murine Giardiasis

Cited by 11 publications

References 34 publications

Heat‐KilledLactobacillus rhamnosusATCC7469 ImprovedUVB‐InducedPhotoagingViaAntiwrinkleandAntimelanogenesisImpacts

Heat‐KilledLactobacillus rhamnosusATCC7469 ImprovedUVB‐InducedPhotoagingViaAntiwrinkleandAntimelanogenesisImpacts

Heat-Killed Lacticaseibacillus paracasei Ameliorated UVB-Induced Oxidative Damage and Photoaging and Its Underlying Mechanisms

Bile Salt Hydrolases: At the Crossroads of Microbiota and Human Health

Contact Info

Product

Resources

About