Comparative Analysis of Collagen-Containing Waste Biodegradation, Amino Acid, Peptide and Carbohydrate Composition of Hydrolysis Products

Сухих, Станислав; Носкова, Светлана; Иванова, Светлана; Ulrikh, Elena; Izgaryshev, Alexsander; Larichev, T. A.; Kozlova, Oksana; Prosekov, Alexander; Babich, Olga

doi:10.3390/app112311511

Cited by 6 publications

(4 citation statements)

References 51 publications

(67 reference statements)

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“…The decreased viability for the sample Col-NT may be due to the different degradation process and the formation of small peptidic chains that suffocate cells and do not promote proper proliferation. As other authors have mentioned, biodegradation plays a critical role in obtaining a desirable 3D scaffold [48,49].…”

Section: Discussionmentioning

confidence: 93%

Crosslinked Collagenic Scaffold Behavior Evaluation by Physico-Chemical, Mechanical and Biological Assessments in an In Vitro Microenvironment

et al. 2022

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Wound healing-associated difficulties continue to drive biotechnological creativeness into complex grounds. The sophisticated architecture of skin wound sites and the intricate processes involved in the response to the use of regenerative devices play a critical role in successful skin regeneration approaches and their possible outcomes. Due to a plethora of complications involved in wound healing processes as well as the coordination of various cellular mechanisms, biomimetic approaches seems to be the most promising starting ground. This study evaluates the behavior of a crosslinked, porous collagen scaffold obtained by lyophilization and dehydrothermal reticulation (DHT). We address the key physio-chemical and mechanical factors, such as swelling, density and porosity, mechano-dynamic properties, SEM and TG-DSC, as well as important biological outcomes regarding scaffold biocompatibility and cellular metabolic activity, cytokine expression in inflammation, apoptosis and necrosis, as well as hemocompatibility and biodegradation. The mechanical and visco-elastic behavior are correlated, with the samples found to present similar thermal behavior and increased rigidity after DHT treatment. High biocompatibility rates were obtained, with no inflammatory stimulation and a reduction in necrotic cells. Higher percentages of cellular early apoptosis were observed. The hemocompatibility rate was under 2%, coagulation effects expressed after 4 min, and the DHT scaffold was more resistant to the biodegradation of collagenase compared with the untreated sample.

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Section: Discussionmentioning

confidence: 93%

Crosslinked Collagenic Scaffold Behavior Evaluation by Physico-Chemical, Mechanical and Biological Assessments in an In Vitro Microenvironment

et al. 2022

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“…Due to the fact that proteases are specific and efficient, the enzyme method is the most popular method of collagen production. The most-used enzymes include pepsin, trypsin, neutral protease, pineapple protease, and papain [31]. Pepsin is the most commonly used enzyme.…”

Section: Methodsmentioning

confidence: 99%

Development of a New Collagen Gel Product for Leather Finishing

Zhang,

Sorolla,

Casas

et al. 2023

Gels

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Leather finishing is a critical process in the leather industry, as it significantly influences the final appearance, durability, and quality of leather products. Traditional leather finishing techniques often involve the use of synthetic chemicals, which may lead to environmental concerns and potential health hazards. In this study, we investigate the feasibility and effectiveness of a new collagen-based product for leather finishing. Collagen, a natural protein found abundantly in animals, has shown promise as an environmentally friendly and sustainable alternative for leather finishing. The new collagen gel product obtained from bovine hide waste by using an alkaline extraction method with lime was functionalized through an enzymatic treatment that allows to achieve a finishing product suitable for coating formulations, and at the same time, a biodegradable finishing. The collagen gel product was optimized by varying parameters, such as temperature, pH, and enzyme quantity. The optimized collagen gel product exhibits a wide particle size range and retains the triple-helical structure of collagen. The leather samples treated with the collagen gel product show enhanced properties compared to those with conventional finishes. The results show that the collagen gel product enhances water vapor permeability, color stability, and touch in the finishes. However, a low resistance to wet rubbing is obtained; therefore, it is necessary to study how to improve this parameter.

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“…The The GPC method was ineffective in analyzing copolymers synthesized with BQ, DQ, and NQ due to the formation of a cross-linked structure, which is similar to copolymers synthesized with 2,5-DTBQ (Figure 1). After the hydrolysis of copolymers by collagenase, the most effective collagen destruction enzyme [52], a water-insoluble polymer, is isolated from the grafted copolymer in a separate phase during the day, which was filtered and analyzed by NMR and GPC methods. 1 H NMR spectra have shown a wide signal at 3.6 ppm in all samples belonging to the methoxy group of polymethyl methacrylate.…”

Section: Data Availability Statementmentioning

confidence: 99%

Scaffold Chemical Model Based on Collagen—Methyl Methacrylate Graft Copolymers

Kuznetsova,

Gushchina,

Lobanova

et al. 2023

Polymers

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Polymerization of methyl methacrylate (MMA) in aqueous collagen (Col) dispersion was studied in the presence of tributylborane (TBB) and p-quinone: 2,5-di-tert-butyl-p-benzoquinone (2,5-DTBQ), p-benzoquinone (BQ), duroquinone (DQ), and p-naphthoquinone (NQ). It was found that this system leads to the formation of a grafted cross-linked copolymer. The inhibitory effect of p-quinone determines the amount of unreacted monomer, homopolymer, and percentage of grafted poly(methyl methacrylate) (PMMA). The synthesis combines two approaches to form a grafted copolymer with a cross-linked structure—“grafting to” and “grafting from”. The resulting products exhibit biodegradation under the action of enzymes, do not have toxicity, and demonstrate a stimulating effect on cell growth. At the same time, the denaturation of collagen occurring at elevated temperatures does not impair the characteristics of copolymers. These results allow us to present the research as a scaffold chemical model. Comparison of the properties of the obtained copolymers helps to determine the optimal method for the synthesis of scaffold precursors—synthesis of a collagen and poly(methyl methacrylate) copolymer at 60 °C in a 1% acetic acid dispersion of fish collagen with a mass ratio of the components collagen:MMA:TBB:2,5-DTBQ equal to 1:1:0.015:0.25.

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Comparative Analysis of Collagen-Containing Waste Biodegradation, Amino Acid, Peptide and Carbohydrate Composition of Hydrolysis Products

Cited by 6 publications

References 51 publications

Crosslinked Collagenic Scaffold Behavior Evaluation by Physico-Chemical, Mechanical and Biological Assessments in an In Vitro Microenvironment

Crosslinked Collagenic Scaffold Behavior Evaluation by Physico-Chemical, Mechanical and Biological Assessments in an In Vitro Microenvironment

Development of a New Collagen Gel Product for Leather Finishing

Scaffold Chemical Model Based on Collagen—Methyl Methacrylate Graft Copolymers

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