Comparative analysis of cutaneous bacterial communities of farmed<i> Rana dybowskii</i> after gentamycin bath

Jia, Bingrui; Tong, Qing; Liu, Xiaoning; Zhang, Xianhao; Wang, Hongbin

doi:10.7717/peerj.8430

Cited by 9 publications

(17 citation statements)

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“…Providing medicine in a bath is also a good method for animals. In this study, gentamicin was used at a dose of 20 mg/L, which is close to the dose used in sh seedlings in aquaculture [12]. The drug bath method is non-invasive with less stress to animals than other methods, possibly reducing animal pain and damage to the skin [11].…”

Section: Frogs In An Antimicrobial Bathmentioning

confidence: 99%

“…However, transdermal absorption also involves the passive diffusion of a chemical through the epidermis into the capillaries of the dermis [11]. The gills and skin of amphibians are highly permeable, resulting in their introduction as a remarkable indicator for assessing the harmful effects of pollution [12]. In research, antibiotics are frequently employed to examine the role of the microbiota.…”

Section: Introductionmentioning

confidence: 99%

“…Rana dybowskii (brown frog) is a major aquaculture species with medical and nutritional values in China [18]. The current stagnation of the frog culture industry is mainly caused by diseases, primarily bacterial diseases [12]. If a disease is found during the breeding process of Rana dybowskii, it is di cult to carry out effective treatment [12].…”

Section: Introductionmentioning

confidence: 99%

“…The current stagnation of the frog culture industry is mainly caused by diseases, primarily bacterial diseases [12]. If a disease is found during the breeding process of Rana dybowskii, it is di cult to carry out effective treatment [12]. Mainly, frogs are ingested quickly after the onset of disease, and drugs are di cult to administer orally; furthermore, due to the number of farmed frogs, small individuals and susceptibility to stress, the intramuscular injection and intravenous injection methods are di cult to achieve [12].…”

Section: Introductionmentioning

confidence: 99%

“…If a disease is found during the breeding process of Rana dybowskii, it is di cult to carry out effective treatment [12]. Mainly, frogs are ingested quickly after the onset of disease, and drugs are di cult to administer orally; furthermore, due to the number of farmed frogs, small individuals and susceptibility to stress, the intramuscular injection and intravenous injection methods are di cult to achieve [12]. Therefore, frog disease prevention and appropriate methods of administration are particularly important.…”

Section: Introductionmentioning

confidence: 99%

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Changes in the Gut Microbiota Diversity of Brown Frogs (Rana Dybowskii) After Local Antimicrobial Therapy

Tong

Cui

Jia

et al. 2021

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Background: Amphibians frequently receive an antibiotic bath after feedlot placement to control bacterial diseases. The potential collateral effect of these antibiotics on the frog microbiota is largely unknown. Antibiotics are frequently employed to examine the role of the gut microbiota. Existing research relies mainly on oral antibiotics, but knowledge regarding the effects of antibiotics on the gut microbiota through a bath or local antimicrobial therapies is limited. Results: The gut microbiota of gentamicin, recovered, and control Rana dybowskii groups were compared by Illumina high-throughput sequencing, and the functional profiles were analysed using a phylogenetic investigation of communities by the reconstruction of unobserved states (PICRUSt). Furthermore, the relationship between gut microbiota structures and forecast function compositions was determined. The results showed that the alpha diversity indices were significantly reduced by the gentamicin bath, which significantly changed the composition of the gut microbiota. After 7 days, the gut microbiota was still similar to that during the gentamicin bath. Forty-four indicator species were selected at the genus level, namely, 42 species indicating the control group and 2 species indicating the gentamicin and recovery groups. Potential pathogenic bacteria belonging to Aeromonas, Citrobacter, and Chryseobacterium significantly decreased after the gentamicin bath. The community similarity assays did not show an obvious discrepancy in the functional composition between the gentamicin and control frogs, indicating that the functions of the gut bacterial community were highly redundant. Conclusions: The gentamicin bath significantly reduced the alpha diversity of the gut microbiota of R. dybowskii. Gentamicin significantly changed the structure of the gut microbiota, and the gut microbiotas exhibited weak resilience and did not totally recover after seven days. The gentamicin bath did not change the functional composition of the gut microbiota of R. dybowskii, and there was no significant correlation between the composition of the gut microbiota and the functional composition, illustrating the high intestinal functional redundancy of the frog gut bacterial community. This work offers basic data for upcoming research, including the establishment of the amphibian gut microbiota and local antibiotic administration, and has important implications for aquaculture management and amphibian conservation.

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Section: Frogs In An Antimicrobial Bathmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%