Comparative analysis of cytokine release from epithelial cell cultures of the upper airway

Shiozawa, Akihito; Miwa, Masato; Ono, Noritsugu; Homma, H; Hirotsu, Mikio; Ikeda, Katsuhisa

doi:10.4193/rhin14.078

Cited by 13 publications

(7 citation statements)

References 45 publications

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“…However, the neutrophilic type of infiltration predominates in some nasal polyp patients, especially in Asian and eastern populations. 2 The pathogenesis of PAR is well explained by antigen-specific T helper 2 (Th2) cells and their cytokines, especially interleukin-5 (IL-5) and IL-13, and by the release of enzymes in the late phase of allergic inflammation. 1,3 These enzymes, especially the eosinophil cationic protein (ECP), can damage the nasal epithelial cells and cause localized edema, resulting in the hyperreactivity of the upper airway and remodeling of the nasal mucosa.…”

Section: Introductionmentioning

confidence: 99%

Effects of Fluticasone Furoate Nasal Spray on Parameters of Eosinophilic Inflammation in Patients With Nasal Polyposis and Perennial Allergic Rhinitis

Mirković

Perić

Đurđević

et al. 2017

Ann Otol Rhinol Laryngol

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Section: Introductionmentioning

confidence: 99%

Effects of Fluticasone Furoate Nasal Spray on Parameters of Eosinophilic Inflammation in Patients With Nasal Polyposis and Perennial Allergic Rhinitis

Mirković

Perić

Đurđević

et al. 2017

Ann Otol Rhinol Laryngol

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“…In fact, increased expression of IL-6 messenger RNA in nasal mucosa biopsies of patients suffering from persistent AR has been reported [45], pollen exposure to patients with AR significantly increased IL-6 in nasal secretions [5], and nasal secretions were increased in allergic patients after intranasal administration of IL-6 [39]. In addition, it has been reported that primary cultures of human nasal epithelial cells from patients with AR showed significant upregulation in the release of IL-6 [46, 47].…”

Section: Discussionmentioning

confidence: 99%

Superior effect of MP-AzeFlu than azelastine or fluticasone propionate alone on reducing inflammatory markers

Roca‐Ferrer

Pujols

Pérez-González

et al. 2018

Allergy Asthma Clin Immunol

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BackgroundMP-AzeFlu, intranasal formulation of azelastine hydrochloride (AZE) and fluticasone propionate (FP), is superior to AZE or FP alone for treatment of allergic rhinitis (AR). However, the precise anti-inflammatory mechanism of action of MP-AzeFlu has not been characterized.ObjectiveTo investigate the anti-inflammatory effects of MP-AzeFlu compared with AZE or FP alone in an established in vitro model of eosinophilic inflammation.MethodsNasal mucosal epithelial cells and peripheral blood eosinophils were obtained from human volunteers. Epithelial cells were stimulated with 10% fetal bovine serum (FBS) in the presence of MP-AzeFlu, AZE, or FP (1:102 to 1:105 dilution). Concentrations of interleukin (IL)-6, IL-8, and granulocyte–macrophage colony-stimulating factor (GM-CSF) were measured by ELISA. Eosinophils were incubated in 10% human epithelial cell–conditioned medium (HECM) and survival assessed by trypan blue dye exclusion. Results are expressed as mean ± SEM percentage secretion/survival compared with FBS/HECM (respectively).ResultsFP and MP-AzeFlu (all dilutions) and AZE (1:102) significantly reduced IL-6 secretion and eosinophil survival compared with positive controls. At 1:102 dilution, IL-6 secretion was significantly lower with MP-AzeFlu (38.3 ± 4.2%, compared with FBS = 100%) than with AZE (76.1 ± 4.9%) or FP (53.0 ± 4.9%). At 1:102 dilution, eosinophil survival was significantly lower with MP-AzeFlu at day 3 (17.5 ± 3.0%) and day 4 (2.4 ± 1.4%, compared with HECM = 100%) than with AZE (day 3: 75.2 ± 7.2%; day 4: 44.0 ± 9.7%) or FP (day 3: 38.5 ± 3.5%; day 4: 14.6 ± 4.0%).ConclusionGreater reductions in cytokine secretion and eosinophil survival observed with MP-AzeFlu in vitro may underlie MP-AzeFlu’s superior clinical efficacy vs. AZE or FP alone observed in AR patients.Electronic supplementary materialThe online version of this article (10.1186/s13223-018-0311-4) contains supplementary material, which is available to authorized users.

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“…epithelial and endothelial cells) have been reported to produce neutrophil chemokines in response to inflammatory stimuli or foreign invaders [26]. In CRS, nasal epithelial cells are an important source of neutrophil chemokines as they have been documented to secrete CXCL8 in response to diesel exhaust particle, bacteria, or inflammatory stimuli [27-30]. In addition, CXCL1, CXCL2, and CXCL8 can be produced by fibroblasts [31,32], neutrophils [6] and mast cells [33] in nasal tissues under the stimulation with different triggers.…”

Section: Local Recruitment Of Neutrophils In Crsmentioning

confidence: 99%

Neutrophils as a Protagonist and Target in Chronic Rhinosinusitis

Wang

Liu

2019

Clin Exp Otorhinolaryngol

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Neutrophils have traditionally been acknowledged as the first immune cells that are recruited to inflamed tissues during acute inflammation. By contrast, their importance in the context of chronic inflammation has been studied in less depth. Neutrophils can be recruited and are largely present in the nasal mucosa of patients with chronic rhinosinusitis (CRS) both in Asians and in Caucasians. Increased infiltration of neutrophils in patients with CRS has been linked to poor corticosteroid response and disease prognosis. Meanwhile, tissue neutrophils may possess specific phenotypic features distinguishing them from resting blood counterparts and are endowed with particular functions, such as cytokines and chemokines production, thus may contribute to the pathogenesis of CRS. This review aims to summarize our current understanding of the pathophysiologic mechanisms of CRS, with a focus on the roles of neutrophils. We discuss recruitment, function, and regulation of neutrophils in CRS and outline the potential therapeutic strategies targeting neutrophils.

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Comparative analysis of cytokine release from epithelial cell cultures of the upper airway

Abstract: Epithelial cells derived from AR and CRSwNPs showed up-regulation of secretion of several cytokines/chemokines both in the steady state and after IL-17A stimulation, which may contribute to the inflammatory responses of AR and CRSwNPs.

Cited by 13 publications

References 45 publications

Effects of Fluticasone Furoate Nasal Spray on Parameters of Eosinophilic Inflammation in Patients With Nasal Polyposis and Perennial Allergic Rhinitis

Effects of Fluticasone Furoate Nasal Spray on Parameters of Eosinophilic Inflammation in Patients With Nasal Polyposis and Perennial Allergic Rhinitis

Superior effect of MP-AzeFlu than azelastine or fluticasone propionate alone on reducing inflammatory markers

Neutrophils as a Protagonist and Target in Chronic Rhinosinusitis

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