Comparative analysis of diosgenin in Dioscorea species and related medicinal plants by UPLC-DAD-MS

Abstract

BackgroundDioscorea is a genus of flowering plants, and some Dioscorea species are known and used as a source for the steroidal sapogenin diosgenin. To screen potential resource from Dioscorea species and related medicinal plants for diosgenin extraction, a rapid method to compare the contents of diosgenin in various plants is crucial.ResultsAn ultra-performance liquid chromatography (UPLC) coupled with diode array detection (DAD) and electrospray ionization mass spectrometry (ESI-MS) method was developed for identification and determination of diosgenin in various plants. A comprehensive validation of the developed method was conducted. Twenty-four batches of plant samples from four Dioscorea species, one Smilax species and two Heterosmilax species were analyzed by using the developed method.The present method presented good sensitivity, precision and accuracy. Diosgenin was found in three Dioscorea species and one Heterosmilax species, namely D. zingiberensis, D. septemloba, D. collettii and H. yunnanensis.ConclusionThe method is suitable for the screening of diosgenin resources from plants. D. zingiberensis is an important resource for diosgenin harvesting.

Diosgenin: Recent Highlights on Pharmacology and Analytical Methodology

“…For example, an UPLC-DAD-MS was developed and validated for the identification and determination of diosgenin in several plants, and the presence of this steroid in three Dioscorea species and one species of Heterosmilax was demonstrated. In addition, it was also suggested that D. zingiberensis can be an important diosgenin source [117]. A UHPLC-based technique with evaporative light scattering detection (ELSD) was developed and validated for the determination of eleven steroidal saponins and diosgenin from several species of Dioscorea .…”

UPLC-QTOF-MS identification of metabolites in rat biosamples after oral administration of Dioscorea saponins: A comparative study

“…As diosgenin is the main aglycone of hydrolyzed DNTS, DPTS and DZTS (Tang et al, 2013;Yi et al, 2014b), and in particular, diosgenin have been reported for the anti-myocardial ischemia activity (Jayachandran et al, 2009), we hypothesize that diosgenin, as a metabolite, can be found due to oral administration of these three total saponins; this could constitute part of evidence supporting similarity in efficacy of these three herbs. (2) Are there significant differences in the metabolic profiles and metabolite levels of rat groups administered with saponins from these herbs?…”

Dioscorea nipponica Makino: a systematic review on its ethnobotany, phytochemical and pharmacological profiles

“…4): Dioscin ( 1 ) [9, 13, 33–47], gracillin ( 2 ) [9, 13, 33–36, 40, 42, 45–48], trillin ( 3 ) [9, 14, 36, 42, 44, 45, 49, 50], diosgenin-3- O -[ α - l -rhamnopyranosyl-(1 → 3)- α - l -rhamnopyranosyl-(1 → 4)- α - l -rhamnopyranosyl-(1 → 4)]- β - d -glucopyranoside ( 4 ) [9, 25, 45], diosgenin-3- O -{ α - l -rhamnopyranosyl-(1 → 2)-[ α - l -rhamnopyranosyl-(1 → 3)]}- β - d -glucopyranoside ( 5 ) [25, 48, 51], diosgenin ( 6 ) [9, 36, 38, 42, 45–47, 49, 52], asperin ( 7 ) [9, 13, 45], 25-Δ-spirosta-3,5-diene ( 8 ) [9, 13, 14, 45–47, 53, 54], piscidic acid ( 9 ) [9, 13, 45–47, 55], 3 β ,26-glycol-25( R )-Δ5,20(22)-diene-furostanol-26- O - β - d -glucopyranoside ( 10 ) [40, 45, 56], progenin II ( 11 ) [9, 48, 52], prosapogenin A of dioscin ( 12 , progenin III) [9, 48], pseudo-protodioscin ( 13 ) [9, 39, 40], protodioscin ( 14 ) [9, 36, 39, 40, 42, 48, 52], protogracillin ( 15 ) [9, 36, 40, 42, 45, 48], kikuba-saponin ( 16 ) [9], smilagenone ( 17 ) [45], and epistephanine ( 18 ) [45, 46]. In addition, allantoin, sterols, flavones, resins, polysaccharides and starch have also been identified as constituents in the roots of D. nipp...…”