UPLC-QTOF-MS identification of metabolites in rat biosamples after oral administration of Dioscorea saponins: A comparative study

Tang, Yiqun; Pang, Yuxin; He, Xi-Cheng; Zhang, Yazhou; Zhang, Jianye; Zhao, Zhongzhen; Yi, Tao; Chen, Hubiao

doi:10.1016/j.jep.2015.02.017

Cited by 71 publications

(35 citation statements)

References 18 publications

(23 reference statements)

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“…This study allowed comparing the metabolic profiles of these saponins and diosgenin as well as analyzing the levels of metabolites, by monitoring the chemical profiles of plasma, feces, and urine of rats during 36 hours. Interestingly, it was proven that diosgenin is one of the major metabolites found in plasma and feces (excluding urine) in all examined groups of rats [133]. Furthermore, the UPLC-UV/MS technique was applied to study the in vitro ADME properties of diosgenin and dioscin from Dioscorea villosa , including the stability analysis in biological fluids (gastric and intestinal fluids), intestinal absorption, and metabolic stability.…”

Section: Methodsmentioning

confidence: 99%

Diosgenin: Recent Highlights on Pharmacology and Analytical Methodology

Jesus

Martins

Gallardo

et al. 2016

Journal of Analytical Methods in Chemistry

210

125

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Diosgenin, a steroidal sapogenin, occurs abundantly in plants such as Dioscorea alata, Smilax China, and Trigonella foenum graecum. This bioactive phytochemical not only is used as an important starting material for the preparation of several steroidal drugs in the pharmaceutical industry, but has revealed also high potential and interest in the treatment of various types of disorders such as cancer, hypercholesterolemia, inflammation, and several types of infections. Due to its pharmacological and industrial importance, several extraction and analytical procedures have been developed and applied over the years to isolate, detect, and quantify diosgenin, not only in its natural sources and pharmaceutical compositions, but also in animal matrices for pharmacodynamic, pharmacokinetic, and toxicological studies. Within these, HPLC technique coupled to different detectors is the most commonly analytical procedure described for this compound. However, other alternative methods were also published. Thus, the present review aims to provide collective information on the most recent pharmacological data on diosgenin and on the most relevant analytical techniques used to isolate, detect, and quantify this compound as well.

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Section: Methodsmentioning

confidence: 99%

Diosgenin: Recent Highlights on Pharmacology and Analytical Methodology

Jesus

Martins

Gallardo

et al. 2016

Journal of Analytical Methods in Chemistry

210

125

View full text Add to dashboard Cite

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“…Nishimura et al has reported that Chinese yam contains 1.9% (w/w) of the SDF (31). Proposed biological mechanisms for the Chol-lowering effects of SDF include (1) increased barrier properties of the unstirred layer between micelles and intestinal absorptive cells, (2) molecular associations of SDF and bile salt, and (3) formation of a local SDF matrix that entraps bile salt micelles (32)(33)(34)(35). In addition, we also confirmed using a Megazyme Resistant Starch Assay Kit (Megazyme International Ltd.) that the present freeze-dried Jin contained 45.4% (w/w) resistant starch.…”

Section: Jin Blocks Lipid Absorption In the Intestines And Decreases mentioning

confidence: 99%

Consumption of Japanese Yam Improves Lipid Metabolism in High-Cholesterol Diet-Fed Rats

Kusano

Tsujihara

Masui

et al. 2016

J Nutr Sci Vitaminol

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SummaryWe investigated the effects of dietary Japanese yam (Dioscorea japonica Thunb.) on lipid metabolism. Male Wistar rats (6 wk old) were fed a high-cholesterol diet for 6 wk and then supplemented with 26% of Japanese yam or 0.5% of its constituent diosgenin for a further 4 wk of high-cholesterol feeding (C6-J4 and C6-D4 groups, respectively). In the C6-J4 group, body weight gains significantly decreased, but skeletal muscle fiber sizes in quadriceps significantly increased compared with the other groups. Furthermore, Japanese yam supplementation resulted in the reduction of triglyceride contents in their liver, quadriceps, and intra-abdominal visceral fat. Diosgenin supplementation resulted in an increase in the numbers of skeletal muscle fibers and decrease in the fat accumulations in liver and of the lipid contents in quadriceps. Although quadriceps cholesterol contents decreased concomitantly with increased serum HDL-cholesterol in both the groups, fecal bile acid, fecal cholesterol contents, and fecal weight were higher in the C6-J4 group than in the C6-D4 group. Meanwhile, we demonstrated that Japanese yam inhibited micellar cholesterol solubility in vitro in a concentration-dependent manner. These results suggest that Japanese yam is more effective than diosgenin in reducing fat accumulation and improving cholesterol metabolism during chronic consumption of a high-cholesterol diet.

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“…The precision of the injection was assessed using six replicated analyses of the same urine sample. [ ] The relative standard deviations (R.S.D%) of the retention time and m / z were listed in Table , which could ensure the reliable of this analysis.…”

Section: Methodsmentioning

confidence: 99%

Urinary metabolomics research for Huangqi Jianzhong Tang against chronic atrophic gastritis rats based on 1H NMR and UPLC-Q/TOF MS

Jin

Qin

Zheng

2020

Journal of Pharmacy and Pharmacology

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Objectives As a traditional Chinese medicine (TCM), Huangqi Jianzhong Tang (HQJZ) has a good efficacy in treating chronic atrophic gastritis (CAG). Our objective was to determine its mechanism based on the urine comprehensive metabolome. Methods In the study, a metabolomic approach was applied to reveal the efficacy of HQJZ on the constructed CAG rats coupled with proton nuclear magnetic resonance (1H NMR) and ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry (UPLC‐Q/TOF MS). Key findings The results showed the regulatory effect of HQJZ on urinary metabolism disorder in CAG rats was similar to the positive drug teprenone. Nineteen and 16 potential biomarkers related to CAG were detected by NMR and UPLC‐Q/TOF MS, respectively. Thirty‐two urine metabolites were significantly regulated by HQJZ treatment. Combined with MetPA and partial least square regression analysis (PLS‐RA), three metabolic pathways of valine, leucine and isoleucine, TCA cycle, and glycine, serine and threonine metabolism were the most relevant pathways for HQJZ treatment. Conclusions The main mechanism of HQJZ might be due to the balance of energy consumption, inflammatory inhibition, improvement of the immune system and oxidative stress on the constructed CAG rats. These findings provided comprehensive metabolic information of TCM by parallel measurements by LC‐MS and NMR.

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UPLC-QTOF-MS identification of metabolites in rat biosamples after oral administration of Dioscorea saponins: A comparative study

Cited by 71 publications

References 18 publications

Diosgenin: Recent Highlights on Pharmacology and Analytical Methodology

Diosgenin: Recent Highlights on Pharmacology and Analytical Methodology

Consumption of Japanese Yam Improves Lipid Metabolism in High-Cholesterol Diet-Fed Rats

Urinary metabolomics research for Huangqi Jianzhong Tang against chronic atrophic gastritis rats based on 1H NMR and UPLC-Q/TOF MS

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