Comparative analysis of DNA-based microbial community composition and substrate utilisation patterns of activated sludge microorganisms from wastewater treatment plants operated under different conditions

Matsuda, Masami; Inoue, Daisuke; Anami, Yasutaka; Tsutsui, Hirofumi; Sei, Kazunari; Soda, Satoshi; Ike, Michihiko

doi:10.2166/wst.2010.212

Cited by 21 publications

(20 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The values of H'(T-RFLP) (Blackwood et al, 2007) and H'(Biolog) (Al-Mutairi, 2007) were calculated respectively from T-RF and carbon source utilization profiles as described previously (Matsuda et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…1.3.4.; http://folk.uio.no/ohammer/past). Carbon source utilization profiles and T-RF profiles of 12 MBRs were compared with those of 11 conventional processes obtained in the previous study (Matsuda et al, 2010). The datasets were prepared using the same calculation method as that described by Matsuda et al (2010).…”

Section: Discussionmentioning

confidence: 99%

“…The monthly average values of temperature, levels of dissolved oxygen (DO), and pH in aeration tanks were calculated for the month when sampling was conducted, referring to the monitoring data by the WWTPs. Concentrations of MLSS, dissolved organic carbon (DOC), and total nitrogen (T-N) of influent and effluent samples were measured in the laboratory as described in a previous report (Matsuda et al, 2010). Other parameters such as sludge retention time and biochemical oxygen demand and suspended sludge (BOD-SS) loading were not obtained from some WWTPs.…”

Section: Methodsmentioning

confidence: 99%

“…The methods of DNA extraction, T-RFLP analysis and the Biolog assay were performed as described elsewhere (Matsuda et al, 2010). For T-RFLP analysis, PCR products of the conserved region of eubacterial 16S rRNA gene were digested with HhaI, HaeIII or MspI, denatured at 92°C for 3 min, and subjected to capillary electrophoresis.…”

Section: T-rflp Analysis and Biolog Assaysmentioning

confidence: 99%

“…The Biolog assay is a culture-dependent technique to characterize the carbon-utilization potentials of the microbial community (Garland and Mills, 1991). Furthermore, using cluster analysis and principal component analysis (PCA), the obtained results were compared to those of 11 conventional processes that were previously reported by Matsuda et al (2010).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Characterization of Microbial Community in Membrane Bioreactors Treating Domestic Wastewater

Takada

Hashimoto

Soda

et al. 2014

J. of Wat. ^|^ Envir. Tech.

View full text Add to dashboard Cite

Activated sludge microbial communities of 12 membrane bioreactors (MBRs) treating domestic wastewater were characterized in terms of their bacterial phylogenic composition and organic metabolism using eubacterial 16S rRNA gene-based terminal-restriction fragment length polymorphism (T-RFLP) analysis and carbon-utilization tests (Biolog assay). The phylogenic microbial structure of MBR sludges differed considerably among samples, depending neither on influent characteristics nor on operational modes and conditions. Comparison of the microbial community structures of the 12 MBRs and 11 conventional processes characterized in a previous study indicated that MBRs and the conventional processes differ considerably in terms of phylogenic structure. Also, dominant bacteria in MBRs differed considerably from each other, although those in the conventional processes were similar. In contrast, the carbon-utilization profile of activated sludge samples of 8 among 12 MBRs and all conventional processes tested in the previous study were mutually similar, indicating that activated sludge communities possess similar biodegradation potential against organic pollutants even if the phylogenetic bacterial composition differs. Results also demonstrated that some MBR sludge samples have unique carbon-utilization profiles, suggesting that MBRs enrich bacterial populations with unique metabolic activity.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: T-rflp Analysis and Biolog Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Characterization of Microbial Community in Membrane Bioreactors Treating Domestic Wastewater

Takada

Hashimoto

Soda

et al. 2014

J. of Wat. ^|^ Envir. Tech.

View full text Add to dashboard Cite

show abstract

Impacts of gene bioaugmentation with pJP4-harboring bacteria of 2,4-D-contaminated soil slurry on the indigenous microbial community

et al. 2011

View full text Add to dashboard Cite

Gene bioaugmentation is a bioremediation strategy that enhances biodegradative potential via dissemination of degradative genes from introduced microorganisms to indigenous microorganisms. Bioremediation experiments using 2,4-dichlorophenoxyacetic acid (2,4-D)-contaminated soil slurry and strains of Pseudomonas putida or Escherichia coli harboring a self-transmissible 2,4-D degradative plasmid pJP4 were conducted in microcosms to assess possible effects of gene bioaugmentation on the overall microbial community structure and ecological functions (carbon source utilization and nitrogen transformation potentials). Although exogenous bacteria decreased rapidly, 2,4-D degradation was stimulated in bioaugmented microcosms, possibly because of the occurrence of transconjugants by the transfer of pJP4. Terminal restriction fragment length polymorphism analysis revealed that, although the bacterial community structure was disturbed immediately after introducing exogenous bacteria to the inoculated microcosms, it gradually approached that of the uninoculated microcosms. Biolog assay, nitrate reduction assay, and monitoring of the amoA gene of ammonia-oxidizing bacteria and nirK and nirS genes of denitrifying bacteria showed no irretrievable depressive effects of gene bioaugmentation on the carbon source utilization and nitrogen transformation potentials. These results may suggest that gene bioaugmentation with P. putida and E. coli strains harboring pJP4 is effective for the degradation of 2,4-D in soil without large impacts on the indigenous microbial community.

show abstract

Characterization of the genes involved in nitrogen cycling in wastewater treatment plants using DNA microarray and most probable number-PCR

Pang

Matsuda

Kuroda

et al. 2016

Front. Environ. Sci. Eng.

View full text Add to dashboard Cite

Comparative analysis of DNA-based microbial community composition and substrate utilisation patterns of activated sludge microorganisms from wastewater treatment plants operated under different conditions

Cited by 21 publications

References 17 publications

Characterization of Microbial Community in Membrane Bioreactors Treating Domestic Wastewater

Characterization of Microbial Community in Membrane Bioreactors Treating Domestic Wastewater

Impacts of gene bioaugmentation with pJP4-harboring bacteria of 2,4-D-contaminated soil slurry on the indigenous microbial community

Characterization of the genes involved in nitrogen cycling in wastewater treatment plants using DNA microarray and most probable number-PCR

Contact Info

Product

Resources

About