Comparative Analysis of Expressed Sequence Tags in Resistant and Susceptible Ecotypes of Arabidopsis thaliana Infected with Cucumber Mosaic Virus

Ishihara, T.; Sakurai, Nozomu; Sekine, Ken Taro; Hase, S.; Ikegami, Machiko; Shibata, Daisuke; Takahashi, Hideki

doi:10.1093/pcp/pch057

Cited by 49 publications

(47 citation statements)

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“…The EST sequences of soybean transcripts regulated differentially in SMV-infected leaf tissues were cross-compared by BLAST-searching against the sequences of the transcripts that were significantly differentially expressed in response to infection by other ss(+) RNA viruses (Golem & Culver, 2003;Whitham et al, 2003;Ishihara et al, 2004;Marathe et al, 2004;Dardick, 2007;Yang et al, 2007). Sequence searches were performed by using the TBLASTX algorithm with default settings.…”

Section: Methodsmentioning

confidence: 99%

“…The uniEST sequences of 894 significantly differentially expressed (either up-or downregulated, regardless of fold change) soybean transcripts identified in this study were BLAST-searched against all of the differentially regulated transcripts identified in potato in response to infection by three distinct fruit tree viruses, namely PPV, tomato ringspot virus (ToRSV) and Prunus necrotic ringspot virus (Dardick, 2007), and in Arabidopsis in response to infection by CMV (Ishihara et al, 2004;Marathe et al, 2004), TuMV (Yang et al, 2007), TMV (Golem & Culver, 2003) and five positive-sense plant viruses including TuMV, oilseed rape mosaic virus, turnip vein clearing virus, potato virus X and CMV (Whitham et al, 2003). As a result, 107 unique transcripts were identified (see Supplementary Table S5, available in JGV Online).…”

Section: Association Of Upregulated Transcripts In Protein Synthesis mentioning

confidence: 99%

“…Dissecting the plant gene-expression network that occurs in response to virus infection should assist in a better understanding of the infection process and ultimately in the control of plant viruses. To achieve this, microarray technology has been adopted to profile global gene expression of the host (mainly model plant species) infected with several positive-sense RNA viruses (Golem & Culver, 2003;Whitham et al, 2003;Ishihara et al, 2004;Marathe et al, 2004;Pompe-Novak et al, 2005;Senthil et al, 2005;Dardick, 2007;Shimizu et al, 2007;Yang et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Association of the transcriptional response of soybean plants with soybean mosaic virus systemic infection

Babu

Gagarinova

Brandle

et al. 2008

Journal of General Virology

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Compatible virus infection induces and suppresses host gene expression at the global level. These gene-expression changes are the molecular basis of symptom development and general stress and defence-like responses of the host. To assess transcriptional changes in soybean plants infected with soybean mosaic virus (SMV), the first soybean trifoliate leaf, immediately above the SMV-inoculated unifoliate leaf, was sampled at 7, 14 and 21 days post-inoculation (p.i.) and subjected to microarray analysis. The identified changes in gene expression in soybean leaves with SMV infection at different time points were associated with the observed symptom development. By using stringent selection criteria (¢2-or ¡"2-fold change and a Q value of ¡0.05), 273 (1.5 %) and 173 (0.9 %) transcripts were identified to be up-and downregulated, respectively, from 18 613 soybean cDNAs on the array. The expression levels of many transcripts encoding proteins for hormone metabolism, cell-wall biogenesis, chloroplast functions and photosynthesis were repressed at 14 days p.i. and were associated with the highest levels of viral RNA in the host cells. A number of transcripts corresponding to genes involved in defence were either downregulated or not affected at the early stages of infection, but upregulated at the late stages, indicating that the plant immune response is not activated until the late time points of infection. Such a delayed defence response may be critical for SMV to establish its systemic infection.

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Section: Methodsmentioning

confidence: 99%

Section: Association Of Upregulated Transcripts In Protein Synthesis mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Association of the transcriptional response of soybean plants with soybean mosaic virus systemic infection

Babu

Gagarinova

Brandle

et al. 2008

Journal of General Virology

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“…Hybridization of the DNA array filters with labeled cDNA targets prepared from total RNA was carried out as described previously (Ishihara et al 2004) with minor modifications. Total RNA (5 mg) was suspended in 8.8 ml of deionized distilled water and mixed with 1 ml of 0.5 mg ml Ϫ1 oligo(dT) [12][13][14][15][16][17][18] .…”

mentioning

confidence: 99%

Non-biased distribution of tomato genes with no counterparts in Arabidopsis thaliana in expression patterns during fruit maturation

Yano

Tsugane

Watanabe

et al. 2006

Plant Biotechnology

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“…Estudos da resposta global de genótipos resistente e suscetível de citros ao CiLV-C -IBANEZ et al, 2008;BRADER et al, 2007;GOLEM;CULVER, 2003;HUANG et al, 2005;ISHIHARA et al, 2004;LOVE et al, 2007;MARATHE et al, 2004;POSTNIKOVA;NEMCHINOV, 2012;RALHAN et al, 2012;TRINKS et al, 2005;WHITHAM et al, 2003;YANG et al, 2007 …”

Section: Interação Planta-patógeno-vetor No Patossistema Leproseunclassified

Análise da expressão gênica de Arabidopsis thaliana em resposta ao Citrus leprosis virus C e ao seu vetor Brevipalpus phoenicis

Arena¹

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Gene expression analysis of Arabidopsis thaliana in response to Citrus leprosis virus C and its vector Brevipalpus phoenicisCitrus leprosis, the main viral disease affecting citrus orchards in Brazil, is caused by Citrus leprosis virus C (CiLV-C, genus Cilevirus). CiLV-C has a bipartite genome of singled stranded positive RNA, which encodes six proteins. CiLV-C is plant-to-plant transmitted by Brevipalpus phoenicis mites and can infect more than 40 plant species, invariably producing localized chlorotic or necrotic lesions around the site of feeding of the viruliferous mites. Viral long distance movement in its hosts is not accomplished. To unveil the mechanisms determining the unique characteristic of the virus/mite/plant interaction, activities of main plant defense pathways were evaluated during aviruliferous and CiLV-C viruliferous mite infestation in Arabidopsis thaliana. The expression of 19 marker genes involved in defense responses along a time course experiment (6, 12 and 24 hours after infestation, and after appearance of leprosis symptoms) was assessed by RT-qPCR. Analyses showed that genes involved in the salicylic acid (SA) pathway were up-regulated during plant interaction with mite and virus. The SA pathway expression profile observed at the infestation by viruliferous mites resembled those observed for the aviruliferous mites, but plant response to both stimuli was stronger. Both the jasmonic acid and ethylene pathways were activated during mite/plant interaction and were repressed at the course of infection with CiLV-C, suggesting an antagonistic effect mediated by the activated SA pathway. Gene silencing mechanism was differentially regulated in response to both aviruliferous and viruliferous mites. Upon viral infection, plants responded with the activation of an AGO1-mediated first defense line, in early times of infection; and then switched to an AGO2-mediated defense. Results indicate the activation of a multifactorial process in response to CiLV-C and B. phoenicis mites in A. thaliana.

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Comparative Analysis of Expressed Sequence Tags in Resistant and Susceptible Ecotypes of Arabidopsis thaliana Infected with Cucumber Mosaic Virus

Cited by 49 publications

References 47 publications

Association of the transcriptional response of soybean plants with soybean mosaic virus systemic infection

Association of the transcriptional response of soybean plants with soybean mosaic virus systemic infection

Non-biased distribution of tomato genes with no counterparts in Arabidopsis thaliana in expression patterns during fruit maturation

Análise da expressão gênica de Arabidopsis thaliana em resposta ao Citrus leprosis virus C e ao seu vetor Brevipalpus phoenicis

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