In this research we have used Windows API (Win-API) call sequences to capture the behaviour of malicious applications. Detours library by Microsoft has been used to hook the Win-APIs call sequences. To have a higher level of abstraction, related Win-APIs have been mapped to a single category. A total set of 534 important Win-APIs have been hooked and mapped to 26 categories (A.. . Z). Behaviour of any malicious application is captured through sequence of these 26 categories of APIs. In our study, five classes of malware have been analyzed: Worm, Trojan-Downloader, Trojan-Spy, Trojan-Dropper and Backdoor. 400 samples for each of these classes have been taken for experimentation. So a total of 2000 samples were taken as training set and their API call sequences were analyzed. For testing, 120 samples were taken for each class. Fuzzy hashing algorithm ssdeep was applied to generate fuzzy hash based signature. These signatures were matched to quantify the API call sequence homologies between test samples and training samples. Encouraging results have been obtained in classification of these samples to the above mentioned 5 categories. Further, N-gram analysis has also been done to extract different API call sequence patterns specific to each of the 5 categories of malware.