Comparative Analysis of Metal Nanoparticles Synthesized From Hibiscus Rosa Sinesis and Their Antibacterial Activity Estimation Against Nine Pathogenic Bacteria

Abstract

Objective: This study demonstrates a simple, cost-effective protocol for biosynthesis of stable silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) from Hibiscus rosa-sinesis and their comparison by applying antibacterial activities against nine pathogenic bacterial species.Methods: AgNPs and AuNPs were biosynthesized from H. rosa-sinesis. The antibacterial activities of biosynthesized AgNPs (bio-AgNPs) and biosynthesized AuNPs (bio-AuNPs) were evaluated against 9 pathogenic bacterial species Pseudomonas aeruginosa, Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis, Enterobacter aerogenes, Escherichia coli, Streptococcus pneumoniae, Aeromonas hydrophila by the agar disc diffusion method.Results: Biological synthesized AgNP/AuNPs were characterized by an ultraviolet-visible (UV-Vis) spectrophotometer, Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). The existence of silver and gold ions in the extracts was conformed (primary confirmation) through UV-Vis and the maximum plasmon peak was observed at 402 and 530 nm of bio-AgNPs and bio-AuNPs, respectively. Bio-AgNPs were obtained in 13.01-28.14 nm size range, whereas bio-AuNPs were in 6.32-18.19 nm size range after analysis of TEM images. The results of FTIR spectra indicate that the bio-AuNPs were bound to amine groups and bio-AgNPs to carboxylate ion groups. The antibacterial activities of AgNP/AuNP, the zone of inhibition significantly increased with the increases of concentrations of bio-AgNPs in all pathogenic bacterial species except in the case of S. epidermidis at 50%, Streptococcus aerogenes and A. hydrophila at 70%, whereas in the case of bio-AuNPs antibacterial activities were displayed only against B. subtilis at 20% and 100% concentration. Conclusion:This study suggests that AgNPs exhibits outstanding antibacterial activity against pathogenic bacteria as compared to AuNPs synthesized from H. rosa-sinensis leaf extract and insights to their potential applicability as an alternative antibacterial agent in microbial and human health system to reduce the resistance ability of pathogenic bacteria.

Study of Antibacterial activity of Phyllanthus emblica and its role in Green Synthesis of Silver Nanoparticles

“…However, the antimicrobial activity is not due to a single compound, but a combination of the metabolites. Similarly, the crude APE was found to contain a myriad of phytochemicals like flavonoids, tannins, triterpenoids and anthraquinones, which is in corroboration with the study by Hutchings, et al 4 . The findings suggested a potent antimicrobial activity by APE.…”

“…The spectrum was recorded throughout a range of 300-700 nm, and the wavelength corresponding to maximum absorption was determined. The shape and particle size of AgNPs was studied by Field emission gunscanning electron microscopy (FEG-SEM) 4 . The colloidal solution of AgNPs made from P. emblica extract was analyzed under UV-Vis spectroscopic technique and the plasmon peak was recorded.…”

“…NPs have a unique chemical and physical properties as compared to their solid bulk materials because of their high surface area and electronic properties. Nanotechnology can be implemented as preventives, diagnostics, drug carriers, antibacterial coatings for implantable devices, in development of antibacterial vaccines and synergetics in the antibacterial therapies 4,5 .…”

Green Synthesis of Iron Nanoparticles from Spinach Leaf and Banana Peel Aqueous Extracts and Evaluation of Antibacterial Potential

“…Using different plant extracts, similar iron NP results were obtained [44][45][46][47][48][49][50]. Despite being much larger than the 50 nm for FeNPs synthesized from tea extracts [34,[51][52][53] and Couroupita guianensis (cannonball tree), the 17 nm NPs were obtained [54], and this size range was more similar in size to NPs synthesized from Damask rose, with average sizes of 100 nm; Thymus vulgaris (garden thyme); and Urtica dioica (nettle leaf) [55].…”

Ascorbic Acid and Polyphenols Mediated Green Synthesis of Silver Nanoparticles from Tagetes erecta L. Aqueous Leaf Extract and Studied Their Antioxidant Properties

“…Literatures available on different platforms regarding silver nanoparticle synthesis from biological material include plant leaf extracts from Azadirachta indica [13], Cassia tora [14], Dracocephalum moldavica [15], Elephantopus scaber [16], and Ziziphora tenuior [17]. The various parts of the plant extract [18,19], black tea leaf [20][21][22], seeds like Mucuna pruriens [23], citrus fruits (Citrus limon, Citrus reticulata, and Citrus sinensis) aqueous extract of mushroom-mediated green synthesis [24,25], and also flowers like Nyctanthes arbortristis [26] play a role as reductase and reduction of silver ions into silver nanoparticles [27]. Further, the application of silver nanoparticles is not limited to one area only [28].…”