Comparative analysis of microRNAs from the lungs and trachea of dogs (Canis familiaris) infected with canine influenza virus

Zhao, Furong; Su, Shuo; Zhou, Dong‐Hui; Zhou, Pei; Xu, Ting-Chuan; Zhang, Liangquan; Cao, Nan; Qi, Wenbao; Zhang, Guihong

doi:10.1016/j.meegid.2013.11.019

Cited by 21 publications

(27 citation statements)

References 56 publications

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“…Besides, cfa-miR-99a, cfa-miR-30 family (including cfa-miR-30a and cfa-miR-30d), cfa-miR-26 family (including cfa-miR-26a and cfa-miR-26b) and cfa-miR-7 family (including cfa-miR-7 and cfa-miR-7g) were the miRNAs of highest activity in dog adrenal cortex. These observations in pituitary and adrenal cortex were distinct from those in dog lung and dog trachea, in which the cfa-miRNA-143 and the cfa-let-7 were the ones of highest activity ( Zhao et al, 2014 ) respectively. Furthermore, when we compared the miRNA expression profiles of pre- and post-stress exposure groups with that of their corresponding gene expression profiles, especially in pituitary, a strikingly converse expression pattern was observed between the miRNA expression profiles and gene expression profiles, indirectly proving that the negatively regulated pattern between miRNAs and genes existed.…”

Section: Discussionmentioning

confidence: 70%

“…However, especially in dogs, the knowledge of the miRNAs associated with stress response in HPA axis is still devoid of a systemic revelation. In addition, to our knowledge, the systemic study of miRNAs in dog tissues still remains poor compared with that in other frequently-used medical animals, just with a study concerning dog tracheas and dog lungs reported ( Zhao et al, 2014 ). In 2008, 357 candidate miRNAs in the Canis familiaris genome were identified with a comparative analysis of the whole genome in silico.…”

Section: Introductionmentioning

confidence: 99%

“…High-throughput sequencing has been a powerful method to investigate the expression profiles of miRNAs, and has been widely utilized in various organisms ( Zhao et al, 2014 ; Zhan et al, 2014 ; Li et al, 2015a ; Wongwarangkana et al, 2015 ). In this study, we aimed to investigate the miRNAs involving chronic stress response in dog pituitary and dog adrenal cortex (pituitary–adrenal axis).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of miRNA expression profiles in pituitary–adrenal axis between Beagle and Chinese Field dogs after chronic stress exposure

Luo

Fang

et al. 2016

PeerJ

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MicoRNAs (miRNAs), usually as gene regulators, participate in various biological processes, including stress responses. The hypothalamus–pituitary–adrenal axis (HPA axis) is an important pathway in regulating stress response. Although the mechanism that HPA axis regulates stress response has been basically revealed, the knowledge that miRNAs regulate stress response within HPA axis, still remains poor. The object of this study was to investigate the miRNAs in the pituitary and adrenal cortex that regulate chronic stress response with high-throughput sequencing. The pituitary and adrenal cortex of beagles and Chinese Field dogs (CFD) from a stress exposure group (including beagle pituitary 1 (BP1), CFD pituitary 1 (CFDP1), beagle adrenal cortex 1 (BAC1), CFD adrenal cortex 1 (CFDAC1)) and a control group (including beagle pituitary 2 (BP2), CFD pituitary 2 (CFDP2), beagle adrenal cortex 2 (BAC2), CFD adrenal cortex 2 (CFDAC2)), were selected for miRNA-seq comparisons. Comparisons, that were made in pituitary (including BP1 vs. BP2, CFDP1 vs. CFDP2, BP1 vs. CFDP1 and BP2 vs. CFDP2) and adrenal cortex (including BAC1 vs. BAC2, CFDAC1 vs. CFDAC2, BAC1 vs. CFDAC1 and BAC2 vs. CFDAC2), showed that a total of 39 and 18 common differentially expressed miRNAs (DE-miRNAs) (Total read counts > 1,000, Fold change > 2 & p-value < 0.001), that shared in at least two pituitary comparisons and at least two adrenal cortex comparisons, were detected separately. These identified DE-miRNAs were predicted for target genes, thus resulting in 3,959 and 4,010 target genes in pituitary and adrenal cortex, respectively. Further, 105 and 10 differentially expressed genes (DEGs) (Fold change > 2 & p-value < 0.05) from those target genes in pituitary and adrenal cortex were obtained separately, in combination with our previous corresponding transcriptome study. Meanwhile, in line with that miRNAs usually negatively regulated their target genes and the dual luciferase reporter assay, we finally identified cfa-miR-205 might play an important role by upregulating MMD in pituitary and hippocampus, thus enhancing the immune response, under chronic stress exposure. Our results shed light on the miRNA expression profiles in the pituitary and adrenal cortex with and without chronic stress exposure, and provide a new insight into miR-205 with its feasible role in regulating chronic stress in the pituitary and hippocampus through targeting MMD.

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Section: Discussionmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of miRNA expression profiles in pituitary–adrenal axis between Beagle and Chinese Field dogs after chronic stress exposure

Luo

Fang

et al. 2016

PeerJ

View full text Add to dashboard Cite

show abstract

“…However, especially in dog, the knowledge of the miRNAs associated with stress response in HPA axis is still devoid of a systemic revelation. In addition, to our knowledge, the systemic study of miRNAs in dog tissues still remains poor compared with that in other frequently-used medical animals, just with a study concerning dog trachea and dog lung reported (Zhao et al 2014). In 2008, 357 candidate miRNAs in the Canis familiaris genome were identified with a comparative analysis of the whole genome in silico.…”

Section: Introductionmentioning

confidence: 99%

“…High-throughput sequencing has been a powerful method to investigate the expression profiles of miRNAs, and has been widely utilized in various organisms (Zhao et al 2014;Zhan et al 2014;Wongwarangkana et al 2015). In this study, we aimed to investigate the miRNAs involving chronic stress response in dog pituitary and dog adrenal cortex (pituitaryadrenal axis).…”

Section: Introductionmentioning

confidence: 99%

Peer Review #1 of "Comparison of miRNA expression profiles in pituitary–adrenal axis between Beagle and Chinese Field dogs after chronic stress exposure (v0.1)"

2016

View full text Add to dashboard Cite

MicoRNAs (miRNAs), usually as gene regulators, participate in various biological processes, of which stress response is included. Hypothalamus-pituitary-adrenal axis (HPA axis) is an important pathway in regulating stress response. Although the mechanism that HPA axis regulates stress response has been basically revealed, the knowledge that miRNAs regulate stress response within HPA axis, still remains poor. The object of this study was to investigate the miRNAs in pituitary and adrenal cortex that regulated chronic stress response with high-throughput sequencing. The pituitary and adrenal cortex of Beagle and Chinese Field Dog (CFD) from a stress exposure group [including Beagle pituitary 1 (BP1), CFD pituitary 1 (CFDP1), Beagle adrenal cortex 1 (BAC1), CFD adrenal cortex 1 (CFDAC1)] and a control group [including Beagle pituitary 2 (BP2), CFD pituitary 2 (CFDP2), Beagle adrenal cortex 2 (BAC2), CFD adrenal cortex 2 (CFDAC2)], were selected for miRNA-seq comparisons. Comparisons, that were made in pituitary (including BP1 vs. BP2, CFDP1 vs. CFDP2, BP1 vs. CFDP1 and BP2 vs. CFDP2) and adrenal cortex (including BAC1 vs. BAC2, CFDAC1 vs. CFDAC2, BAC1 vs. CFDAC1 and BAC2 vs. CFDAC2), showed that a total of 39 and 18 common differentially expressed miRNAs (DE-miRNAs) (Total read counts >1000, Fold change >2 & P-value <0.001), that shared in at least two pituitary comparisons and at least two adrenal cortex comparisons, were detected separately. These identified DE-miRNAs were predicted for target genes, thus resulting in 3959 and 4010 target genes in pituitary and adrenal cortex, respectively. Further, 105 and 10 differentially expressed genes (DEGs) (Fold change >2 & P-value <0.05) from those target genes in pituitary and adrenal cortex were obtained separately, in combination with our previous corresponding transcriptome study. Meanwhile, in line with that miRNAs usually negatively regulated their target genes and the dual luciferse reporter assay, we finally identified cfa-miR-205 might play an important role by up-regulating MMD in pituitary and hippocampus, thus enhancing the immune response, under chronic stress exposure. Our results shed light on the miRNA expression profiles in pituitary and adrenal cortex with and without chronic stress exposure, and provide a new insight into miR-205 with its feasible role in regulating chronic stress in pituitary and hippocampus through targeting MMD.

show abstract

Identification of differentially expressed microRNAs in the skin of experimentally sensitized naturally affected atopic beagles by next-generation sequencing

et al. 2020

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Comparative analysis of microRNAs from the lungs and trachea of dogs (Canis familiaris) infected with canine influenza virus

Cited by 21 publications

References 56 publications

Comparison of miRNA expression profiles in pituitary–adrenal axis between Beagle and Chinese Field dogs after chronic stress exposure

Comparison of miRNA expression profiles in pituitary–adrenal axis between Beagle and Chinese Field dogs after chronic stress exposure

Peer Review #1 of "Comparison of miRNA expression profiles in pituitary–adrenal axis between Beagle and Chinese Field dogs after chronic stress exposure (v0.1)"

Identification of differentially expressed microRNAs in the skin of experimentally sensitized naturally affected atopic beagles by next-generation sequencing

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