Comparative Analysis of Perspective Extragation Methods for Receiving Extractions from Fenugreek Seeds

Abstract

Introduction. Today, innovative technologies are widely used in pharmacology (in particular, in the production of herbal preparations), in cosmetology (obtaining various extracts and oils, complex preparations), as well as in the food industry (as natural dyes, etc.). Plant extracts with a high level of risk do not have a harmful effect on the human body, except that they provide environmental safety, which creates a special interest for the pharmaceutical industry . vibrocavitation and supercritical fluid CO2 extraction.Aim. The choice of the optimal level of extraction to obtain a high level of fenugreek seeds.Materials and methods. Obtaining a vibrocavitation extract with an experimental vibration-explosive installation made at the Department of Processes and Apparatus of the St. Petersburg State Technology Institute. Extracts at a temperature of 60±2 ºС. The frequency of revolutions of the homogenizer ranged from 1000 to 5000 rpm. Ultrasonic impact using ultrasound unit I100-6/4 Ultrasonic effect on solid plant material with an intensity of 22 kHz for 60 minutes. The time of extraction in a vibro-cavitation extractor-homogenizer was studied for optimal values.Supercritical fluid CO2-extraction is obtained in two versions with the use of extragent (96% ethanol in the presence of carbon dioxide: ethanol 9:1) and without it. Extraction of the sound of a supercritical fluid extraction system with a 1-liter vessel. SFE1000-2-BASE with a kit for upgrading the SFE1000-2-BASE system to an SFE1000M1-2-FMC50 system (Waters, USA). The flow rate of the extractant was 60 g/min. Emergency listening for one hour and pressure 200, 300 and 400 bar. Extraction is observed three times. The obtained extracts indicate the amount of extractive substances according to article of Russian state pharmacopoeia 1.5.3.0006.15 «Determination of the content of extractive substances in plant raw materials and medicinal plant preparations». Quantitative determination of the saponin complex of parasitic seed seeds on an Agilent QTOF-6530 chromatograph with two ESI and APCI ionization sources according to Gravel et al.Results and discussion. Studies have allowed to determine the amount of extractives in the seeds and choose the most promising method. Conclusion. As a result of our research, it was found that the most promising extraction method for extracting seeds is a parasitic with vibration extraction at frequent revolutions of the homogenizer of 5000 rpm and an extraction time of 60 minutes.

The Effects of Selected Extraction Methods and Natural Deep Eutectic Solvents on the Recovery of Active Principles from Aralia elata var. mandshurica (Rupr. & Maxim.) J. Wen: A Non-Targeted Metabolomics Approach

“…When compressed again, these bubbles collapse, developing a pressure of up to hundreds of atmospheres, resulting in the formation of a highly intense shock wave. This leads to the additional mechanical destruction of solid plant particles and exudes small volumes of liquid from the phase interface, breaking up into small droplets and re-entering the plant [ 7 , 15 , 16 ]. This phenomenon helps to understand the high efficiency of NADES extraction using VAE.…”

“…Before vibrocavitation-assisted extraction, the plant material was soaked with the NADES for 15 min and then transferred to the extractor. The vibrocavitator (laboratory sample of the device developed at St. Petersburg State Technical University, Russia) was applied at 50 Hz for 5 min [ 16 ]. The corresponding instrumentation is presented in Figure 8 .…”

“…This approach relies on the combination of the cavitation effect in the liquid phase with the intensive grinding of plant material due to cutting off plant particles in the gap between the rotor and the stator [ 15 ]. For example, the recovery of dioscin from the seeds of Trigonella foenum graecum L. increased in comparison to the maceration-based procedure when vibrocavitation-assisted extraction (VAE) was applied [ 16 ]. However, the efficiency of the vibrocavitation technique for the extraction of plant materials with NADESs has not been addressed so far.…”

Kinetics of Extraction of Dioscine from Vegetable Raw Materials in Vibrocavitation Homogenizer

“…Очевидно, что это связано с постоянством зазора между статором и ротором виброкавитационного гомогенизатора. Необходимо отметить, что при экстрагировании другими методами -в аппаратах с мешалкой; с использованием ультразвука и низкотемпературной СО 2 -экстракцией [1] дисперсный состав семян пажитника также подвергался измельчению и средний размер частиц во всех опытах был близким к указанному. Это позволяет проводить сравнение различных методов экстрагирования по эффективности друг с другом.…”

“…Извлечения ценных веществ из растительного сырья производится с помощью процесса экстрагирования, который осуществляется различными методами и в аппаратах различной конструкции. Ранее [1] было проведено сравнительное исследование экстрагирования диосцина из пажитника сенного различными методами: в аппаратах с мешалкой; в ультразвуковом поле; сверхкритической, флюидной СО 2 экстракцией и в виброкавитационном гомогенизаторе. Показано, что наиболее эффективным методом является экстрагирование, осуществляемое в виброкавитационном гомогенизаторе.…”