Comparative analysis of physical stress responses in soybean seedlings using cloned heat shock cDNAs

Abstract

Soybean seedlings were subjected to a wide range of physical (abiotic) or environmental stresses. Cloned cDNAs to heat shock (hs)-induced mRNAs were used to assess whether these diverse stresses induced the accumulation of poly(A)RNAs in common with those induced by hs. Northern blot hybridization analyses indicated that a wide range of stress agents lead to the accumulation of detectable levels of several of the hs-induced poly(A)RNAs; the relative concentration of those RNAs 'induced' by the wide range of stress agents (e.g. water stress, salt stress, anaerobiosis, high concentrations of hormones, etc.), was generally in the order of 100-fold lower than that induced by hs. There are two notable exceptions to that pattern of response to the stress agents. First, arsenite treatment resulted in accumulation of the 'hs poly(A)RNAs' to levels similar to those induced by hs. Cadmium also induced a somewhat normal spectrum of the 'hs poly(A)RNAs', but generally lower levels accumulated than in hs- and arsenite0treated tissues. Second, one set of poly(A)RNAs which are present at low and variable levels in control (non-stressed tissue) tissue, and which are increased some 5- to 10-fold by hs, increased in relative concentration in response to a wide range of the stress agents similarly to the response to hs. The physiological significance of the accumulation of this set of poly(A)RNAs (which translate into four electrophoretically different 27 kd proteins) is not known, but they certainly seem to serve as a monitor (or barometer) of physiological stress conditions. Cadmium treatment results in the accumulation of those same poly(A)RNAs and an additional band of higher molecular weight poly(A)RNA homologous to the same hs cDNA clone (clone pCE 54). Ethylene seems to have no obvious causal relationship to the hs response, even though hs-treated seedlings display some symptoms similar to those exhibited by ethylene-treated seedlings.

Expression and RNA Splicing of the Maize Glutathione S-Transferase Bronze2 Gene Is Regulated by Cadmium and Other Stresses

“…The 14.1-kD alternative protein, still containing the GSHbinding site encoded within the first exon of Bz2, could bind the GSH moiety of a PC-cadmium complex, perhaps assisting in the sequestration of PC-cadmium complexes to the vacuole through the heavy metal transporter (HMT1) shown to be responsible for PC-cadmium transport into vacuoles (Ortiz et al, 1995). Previous investigators have speculated that truncated proteins could be produced from the unprocessed versions of the hsp70 and GmGST26-A messages (Czarnecka et al, 1984(Czarnecka et al, , 1988Winter et al, 1988). Czarnecka et al (1984) showed that both a 26-kD, full-length GmGST26-A protein and a 13-to 15-kD truncated protein could be in vitro translated during cadmium stress.…”

“…Previous investigators have speculated that truncated proteins could be produced from the unprocessed versions of the hsp70 and GmGST26-A messages (Czarnecka et al, 1984(Czarnecka et al, , 1988Winter et al, 1988). Czarnecka et al (1984) showed that both a 26-kD, full-length GmGST26-A protein and a 13-to 15-kD truncated protein could be in vitro translated during cadmium stress. A truncated BZ2 protein produced during cadmium stress from the unspliced Bz2 mRNA is currently being investigated in our laboratory (J.C. Urioste, unpublished results).…”

Guanine nucleotide binding protein involvement in early steps of phytochrome-regulated gene expression.

“…Isolation of RNA from SB-P cells and Northern blot analyses were performed as described (15). 32P-labeled DNA probes were prepared by random-primer labeling of the isolated cDNA inserts of soybean cab and hsp75 and maize 17S rRNA for detection of the soybean 18S rRNA (16)(17)(18) (Fig. 1A).…”

Characterization of the Heat Shock Response in Cultured Sugarcane Cells

“…The HSR of higher plants is similar to that of other organisms. The HSPs have been characterized in great detail in soybean (10,16,17,20), maize (4, 9), tobacco (11,15), and tomato (25). …”

“…The HSR of higher plants is similar to that of other organisms. The HSPs have been characterized in great detail in soybean (10,16,17,20), maize (4,9), tobacco (11,15), and tomato (25). The soybean HSPs are also induced by other forms of stress such as arsenite; however, the proteins are not localized in the cell as they are under heat shock conditions (10).…”