Comparative analysis of <scp>R2R3‐MYB</scp> transcription factors in the flower of <i>Iris laevigata</i> identifies a novel gene regulating tobacco cold tolerance

Yang, Jingli; Song, Yu; Shi, Guannan; Yan, Liying; Lv, Rui; Ma, Zongxin; Wang, L

doi:10.1111/plb.13452

Cited by 10 publications

(7 citation statements)

References 44 publications

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“…For example, MdMYB88/124 can stimulate MdCA1 and MdCOR47 through signal transduction pathways upon receiving low-temperature signals, and MdCBF1/MdCBF3 and other related genes were highly expressed [ 23 ]. Low temperatures can induce the expression of the IlMYB306 gene in Iris laevigata , and the content and activity of various physiologically active substances in tobacco overexpressing IlMYB306 undergo significant changes after low-temperature stress, such as a decrease in chlorophyll content, an increase in free proline content, and a significant increase in activities such as MDA, SOD, and POD [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

FvMYB108, a MYB Gene from Fragaria vesca, Positively Regulates Cold and Salt Tolerance of Arabidopsis

Song,

Yang,

Jiao

et al. 2024

IJMS

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MYB (myoblast) protein comes in large quantities and a wide variety of types and plays a role in most eukaryotes in the form of transcription factors (TFs). One of its important functions is to regulate plant responses to various stresses. However, the role of MYB TFs in regulating stress tolerance in strawberries is not yet well understood. Therefore, in order to investigate the response of MYB family members to abiotic stress in strawberries, a new MYB TF gene was cloned from Fragaria vesca (a diploid strawberry) and named FvMYB108 based on its structural characteristics and evolutionary relationships. After a bioinformatics analysis, it was determined that the gene belongs to the R2R3-MYB subfamily, and its conserved domain, phylogenetic relationships, predicted protein structure and physicochemical properties, subcellular localization, etc. were analyzed. After qPCR analysis of the expression level of FvMYB108 in organs, such as the roots, stems, and leaves of strawberries, it was found that this gene is more easily expressed in young leaves and roots. After multiple stress treatments, it was found that the target gene in young leaves and roots is more sensitive to low temperatures and salt stimulation. After these two stress treatments, various physiological and biochemical indicators related to stress in transgenic Arabidopsis showed corresponding changes, indicating that FvMYB108 may be involved in regulating the plant’s ability to cope with cold and high-salt stress. Further research has found that the overexpression of this gene can upregulate the expression of AtCBF1, AtCOR47, AtERD10, and AtDREB1A related to low-temperature stress, as well as AtCCA1, AtRD29a, AtP5CS1, and AtSnRK2.4 related to salt stress, enhancing the ability of overexpressed plants to cope with stress.

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Section: Introductionmentioning

confidence: 99%

FvMYB108, a MYB Gene from Fragaria vesca, Positively Regulates Cold and Salt Tolerance of Arabidopsis

Song,

Yang,

Jiao

et al. 2024

IJMS

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“…To stagger the flowering time and prolong the flowering period, it is necessary to find genes that can advance or delay the flowering. I. laevigata is also sensitive to abiotic stress [27]. Thus, extending the flowering period and increasing the resistance to salt and drought stress are two central goals in the field of breeding I. laevigata of novel properties [28,29].…”

Section: Introductionmentioning

confidence: 99%

WRKY22 Transcription Factor from Iris laevigata Regulates Flowering Time and Resistance to Salt and Drought

Fan,

Niu,

Shi

et al. 2024

Plants

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Iris laevigata Fisch. is an excellent ornamental plant in cold regions due to its unique ornamental ability and strong cold resistance. However, the flowering period of the population is only about 20 days, greatly limiting its potential uses in landscaping and the cutting flower industry. In addition, I. laevigata is often challenged with various abiotic stresses including high salinity and drought in its native habitats. Thus, breeding novel cultivars with delayed flowering time and higher resistance to abiotic stress is of high importance. In this study, we utilized sequencing data from the I. laevigata transcriptome to identify WRKYs and characterized IlWRKY22, a key transcription factor that modulates flowering time and abiotic stress responses. IlWRKY22 is induced by salt and drought stress. We cloned IlWRKY22 and found that it is a Group IIe WRKY localized in the nucleus. Overexpressing IlWRKY22 in Arabidopsis thaliana (L.) Heynh. and Nicotiana tabacum L. resulted in a delayed flowering time in the transgenic plants. We created transgenic N. tabacum overexpressing IlWRKY22, which showed significantly improved resistance to both salt and drought compared to the control plants. Thus, our study revealed a unique dual function of IlWRKY22, an excellent candidate gene for breeding novel Iris cultivars of desirable traits.

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“…The large, bright, and elegant flowers, along with tall, verdant, and straight leaves, make I. laevigata of high ornamental values. In addition, I. laevigata is strongly tolerant of cold [ 2 ]. Thus, I. laevigata is an ideal gardening plant in the northeast region of China with high commercial value.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the identity and function of MADS-box genes in I. laevigata have not been reported. To overcome the unavailability of genome data, our group recently performed RNA-sequencing using the flower tissue of I. laevigata [ 2 ]. The valuable resource made it possible to screen 23 R2R3-MYBs genes that are involved in cold-resistance in I. laevigata [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…To overcome the unavailability of genome data, our group recently performed RNA-sequencing using the flower tissue of I. laevigata [ 2 ]. The valuable resource made it possible to screen 23 R2R3-MYBs genes that are involved in cold-resistance in I. laevigata [ 2 ]. Using a similar approach, the aim of this study was to systematically identify MADS-box genes in I. laevigata and characterize selected genes in the context of flowering control.…”

Section: Introductionmentioning

confidence: 99%

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Identification of MADS-Box Transcription Factors in Iris laevigata and Functional Assessment of IlSEP3 and IlSVP during Flowering

Liu

Shi

et al. 2022

IJMS

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Iris laevigata is ideal for gardening and landscaping in northeast China because of its beautiful flowers and strong cold resistance. However, the short length of flowering time (2 days for individual flowers) greatly limits its applications. Molecular breeding and engineering hold high potential for producing I. laevigata of desirable flowering properties. A prerequisite is to identify and characterize key flowering control genes, the identity of which remains largely unknown in I. laevigata due to the lack of genome information. To fill this knowledge gap, we used sequencing data of the I. laevigata transcriptome to identify MADS-box gene-encoding transcription factors that have been shown to play key roles in developmental processes, including flowering. Our data revealed 41 putative MADS-box genes, which consisted of 8 type I (5 Mα and 3 Mβ, respectively) and 33 type II members (2 MIKC* and 31 MIKCC, respectively). We then selected IlSEP3 and IlSVP for functional studies and found that both are localized to the nucleus and that they interact physically in vitro. Ectopic expression of IlSEP3 in Arabidopsis resulted in early flowering (32 days) compared to that of control plants (36 days), which could be mediated by modulating the expression of FT, SOC1, AP1, SVP, SPL3, VRN1, and GA20OX. By contrast, plants overexpressing IlSVP were phenotypically similar to that of wild type. Our functional validation of IlSEP3 was consistent with the notion that SEP3 promotes flowering in multiple plant species and indicated that IlSEP3 regulates flowering in I. laevigata. Taken together, this work provided a systematic identification of MADS-box genes in I. laevigata and demonstrated that the flowering time of I. laevigata can be genetically controlled by altering the expression of key MADS-box genes.

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Comparative analysis of R2R3‐MYB transcription factors in the flower of Iris laevigata identifies a novel gene regulating tobacco cold tolerance

Cited by 10 publications

References 44 publications

FvMYB108, a MYB Gene from Fragaria vesca, Positively Regulates Cold and Salt Tolerance of Arabidopsis

FvMYB108, a MYB Gene from Fragaria vesca, Positively Regulates Cold and Salt Tolerance of Arabidopsis

WRKY22 Transcription Factor from Iris laevigata Regulates Flowering Time and Resistance to Salt and Drought

Identification of MADS-Box Transcription Factors in Iris laevigata and Functional Assessment of IlSEP3 and IlSVP during Flowering

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