Comparative Analysis of the Blood Plasma Metabolome of Negligible, Gradual and Rapidly Ageing Fishes

Abstract

There are a number of different animals that belong to long- and short-lived species and show a various rate of ageing, providing an ideal model to investigate mechanisms of longevity. In this work, a metabolome profiling of blood plasma from fishes with various ageing rates—negligible (Pike Esox Lucius and Sterlet Acipenser ruthenus), gradual (Zander Sander lucioperca and Perch Perca fluviatilis) and rapid (Chum Salmon Oncorhynchus keta and Pink Salmon Oncorhynchus gorbuscha)—was assessed by means of direct infusion to quadrupole time-of-flight mass spectrometry. Of the 2056 distinct m/z features detected by a mass spectrometry metabolic profiling of blood plasma samples, fifteen metabolites in the classes of dipeptides, fatty acids, glycerolipids, phosphoethanolamines and phosphatidylcholines were significantly associated with ageing rate, independent of species differences. This is the first study of the metabolome of fishes with various ageing rate, and this untargeted approach highlighted the metabolic conditions that may serve to assess the ageing process.

Comparative Analysis of Skeletal Muscle Metabolites of Fish with Various Rates of Aging

“…This fact provides the possibility of translating the results onto processes in mammals. This study is a follow-up to a previously published investigation of blood metabolic profiles associated with the various ageing rate of fishes [19]. The research was focused on the analysis of metabolic compositions of fish muscle tissues for the identification of distinctive features between compared groups.…”

Metabolome profiling in the study of aging processes

“…Следует отметить ряд метаболомных исследований старения, выполненных на животных моделях как млекопитающих (мыши, крысы, собаки) [58,78,90,91], так и не относящихся к млекопитающим (позвоночные и беспозвоночные) [55,92,93] (табл. 2).…”

The Potential of Calorie Restriction and Calorie Restriction Mimetics in Delaying Aging: Focus on Experimental Models

“…The fishes include the shortest- and longest-lived vertebrates in nature [ 82 , 83 , 84 ]. The heterogeneity in longevity provides a unique possibility for exploring the molecular mechanisms that determine the differences in the rate of aging by applying comparative studies [ 85 , 86 ].…”