Comparative Analysis of the Concentration of Injected Horseradish Peroxidase in Cytoplasmic Granules of the Kidney Cortex, in the Blood, Urine, and Liver

Straus, Werner

doi:10.1083/jcb.48.3.620

Cited by 23 publications

(2 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is somewhat suprising since lysosomes in tubular cells contain hydrolytic enzymes capable of inactivating peroxidase (25). It is possible that the observed persistence was due to the large dose of the tracer given (26). We were unable to detect any difference in the number of HRP containing tubular cell vacuoles between experimental and control animals.…”

Section: Resultscontrasting

confidence: 45%

Altered Functional Properties of the Renal Glomerulus in Autologous Immune Complex Nephritis

Schneeberger

Leber

Karnovsky

et al. 1974

The Journal of Experimental Medicine

View full text Add to dashboard Cite

The present study was undertaken in order to investigate the nature of the glomerular damage in a form of experimental immune complex nephritis. The model of autologous immune complex nephritis (AIC) 1 (Heymann's nephritis) (1) was chosen for several reasons. First, the model bears a remarkable resemblance, in terms of ultrastructural and immunofluorescence features, to an important renal disease in man, membranous glomerulonephritis. Furthermore, the model is highly reproducible. In addition, the basic pathogenetic mechanism has been thoroughly documented (2-4). Briefly, the disease is produced in rats by immunization with homologous kidney preparations, which results in the formation of autoantibodies against a renal antigen, normally found in the brush border of proximal tubules. This antigen is presumed to enter the circulation in small amounts and combine with autoantibody to form complexes which deposit in glomeruli. 2 The complexes are detected as granular deposits of immunoglobulin and complement along the epithelial side of the glomerular basement membrane. The disease, like its human counterpart, is manifested by proteinuria, which is often heavy.Our approach was to use the enzymatic tracers horseradish peroxidase (HRP) (40,000 daltons) and catalase (240,000 daltons), and the electronopaque tracer ferritin (ca. 500,000 daltons) as probe molecules with which to assess the altered functional properties of the glomerular capillary wall. These ultrastructural tracers can be directly visualized, and their location within the glomerular capillary wall can be followed at sequential intervals after intravenous injection. Such protein tracers have been extensively used to identify * This study was supported by U.S. Public Health Service grants no. AM 16392 and AM 13132.1Abbreviations used in this paper: AIC, autologous immune complex; CFA, complete Freund's adjuvant; CL, capillary lumen; DAB, 3,3' diaminobenzidine-tetra-HC1; GBM, glomerniar basement membrane; HRP, horseradish peroxidase; US, urinary space.It is of interest that a related pathogenetic mechanism may operate in man. It has recently been reported that a tubular-derived antigen may be present in the glomerular immune deposits in some cases of human membranous glomerulonephritis (5).

show abstract

Section: Resultscontrasting

confidence: 45%

Altered Functional Properties of the Renal Glomerulus in Autologous Immune Complex Nephritis

Schneeberger

Leber

Karnovsky

et al. 1974

The Journal of Experimental Medicine

View full text Add to dashboard Cite

show abstract

“…These observations appear to be the morphologic analog of the nephron puncture observations in rats studied, respectively, 4 and 6 days after the adminis tration of aminonucleoside [16]. In general, droplets of both proteins were far more prominent in the luminal than in the basal half of the tubular cells, as might be expected if the droplets containing proteins merged with and became incorporated into the lysosomes [14], Following the injection of horseradish peroxidase, Straus [17] observed increased numbers of peroxidase containing droplets in the ascending limb of the loop of Henle, distal tubules, and collecting ducts. To our knowledge, no nephron puncture data are yet available which bear directly upon the protein content of the distal tubules, at least in disease, and which could provide direct information about protein reabsorption or secretion in the distal tubules.…”

Section: Discussionmentioning

confidence: 68%

Albumin and γ-Globulin in the Nephron of the Normal Rat and Following the Injection of Aminonucleoside

Exaire¹,

Pollak²,

Pesce³

et al. 1972

Nephron

View full text Add to dashboard Cite

To study aspects of protein homeostasis and proteinuria, experiments were done on normal rats and on rats 1–6 days after a single intravenous injection of the aminonucleoside of puromycin. Albumin excretion of normal rats, measured by a sensitive radioimmunoassay, was 0.139 mg/24 h. Following the injection of aminonucleoside, albumin excretion first increased on the second or third day, and increased thereafter exponentially with time. In 0.5 µm frozen substituted sections of kidney tissue there were a few droplets of albumin, but not of γ-globulin, in the proximal tubules of control rats. There was little change one day after aminonucleoside. Large numbers of protein reabsorption droplets, containing albumin and γ-globulin, were seen in both proximal and distal tubular cells of rats 4–6 days after aminonucleoside. This finding is consistent with increased reabsorption from the lumen by both proximal and distal tubules and, inferentially, with increased glomerular permeability to both proteins. Six days after aminonucleoside, two populations of tubules were observed; one relatively normal in appearance and containing an increased number of droplets; the second dilated, and containing massive amounts of both proteins in lumen and cells. This observation suggests some heterogeneity of nephron damage and function following aminonucleoside.

show abstract

The blood‐brain barrier of the rat choroid plexus

Davis

Milhorat

1975

Anat. Rec.

View full text Add to dashboard Cite

Following intravenous injection, cytochrome c traverses the capillary endothelium of the rat choroid plexus and permeates the perivascular space and the extracellular space between epithelial cells. The tracer is incorporated into pinocytotic vesicles adjacent to the lateral and basal plasmalemmas. Thereafter, cytochrome c is incorporated into multivesicular and dense bodies. Tracerladen vesicles were not found to fuse with the apical plasmalemma and cytochrome c was not discharged into the cerebral ventricles. Acid phosphatase activity of the choroidal epithelium after the administration of cytochrome c was greatly increased and localized in the same intracellular sites shown for cytochrome c. These data suggest that cytochrome c and possibly other proteins that penetrate the choroidal stroma are taken up by the choroidal epithelium and subsequently degraded in lysosomal vesicles. This heterolytic mechanism may be an important means for preventing the entry of certain substances such as proteins into CSF and subsequently into nervous tissue.

show abstract

Comparative Analysis of the Concentration of Injected Horseradish Peroxidase in Cytoplasmic Granules of the Kidney Cortex, in the Blood, Urine, and Liver

Cited by 23 publications

References 19 publications

Altered Functional Properties of the Renal Glomerulus in Autologous Immune Complex Nephritis

Altered Functional Properties of the Renal Glomerulus in Autologous Immune Complex Nephritis

Albumin and γ-Globulin in the Nephron of the Normal Rat and Following the Injection of Aminonucleoside

The blood‐brain barrier of the rat choroid plexus

Contact Info

Product

Resources

About