Comparative Analysis of the Humoral Immune Response to Moraxella catarrhalis and Streptococcus pneumoniae Surface Antigens in Children Suffering from Recurrent Acute Otitis Media and Chronic Otitis Media with Effusion

Verhaegh, Suzanne J. C.; Stol, Kim; Vogel, Corné P. de; Riesbeck, Kristian; Lafontaine, Eric R.; Murphy, Timothy F.; Belkum, Alex van; Hermans, Peter W. M.; Hays, John P.

doi:10.1128/cvi.05630-11

Cited by 12 publications

(11 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further studies are proposed to disclose the immune features of OMP CD contributing to these discrepancies. In another study, Verhaegh and co-workers did not find a correlation between the serum and MEF antibody levels to ten recombinant Mcat proteins or Mcat NP colonization and AOM events in children under 5 years old during recurrent AOM and chronic otitis media with effusion [10]. Our observations and those of others indicate that there is a complicated interplay between the host antibody immune response and Mcat acute infections.…”

Section: Discussionsupporting

confidence: 41%

Serum antibody response to Moraxella catarrhalis proteins OMP CD, OppA, Msp22, Hag, and PilA2 after nasopharyngeal colonization and acute otitis media in children

Ren

Almudevar

Murphy

et al. 2015

Vaccine

Self Cite

View full text Add to dashboard Cite

Background There is no licensed vaccine for Moraxella catarrhalis (Mcat), which is a prominent bacterium causing acute otitis media (AOM) in children and lower respiratory tract infections in adults. Nasopharyngeal (NP) colonization caused by respiratory bacteria results in natural immunization of the host. To identify Mcat antigens as vaccine candidates, we evaluated the development of naturally induced antibodies to 5 Mcat surface proteins in children 6–30 months of age during Mcat NP colonization and AOM. Methods Human serum IgG against the recombinant Mcat proteins, outer membrane protein (OMP) CD, oligopeptide permease (Opp)A, hemagglutinin (Hag), Moraxella surface protein (Msp)22, and PilA clade 2 (PilA2) was quantitated by using an ELISA assay. Results There were 223 Mcat NP colonization episodes documented in 111 (60%) of 184 children in the study. Thirty five Mcat AOM episodes occurred in 30 (16%) of 184 children. All 5 Mcat candidate vaccine antigens evaluated stimulated a significant rise in serum IgG levles over time from 6 to 36 months of age (P < 0.001), with a rank order as follows: Msp22 = OppA > OMP CD = Hag = PilA2. Children with no detectable Mcat NP colonization showed a higher serum IgG level against OppA, Hag, and Msp22 compared to those with Mcat NP colonization (P < 0.05). Individual data showed that some children responded to AOM with an antibody increase to one or more of the studied Mcat proteins but some children failed to respond. Conclusions Serum antibody to Mcat candidate vaccine proteins OMP CD, OppA, Msp22, Hag, and PilA2 increased with age in naturally immunized children age 6–30 months following Mcat NP colonization and AOM. High antibody levels against OppA, Msp22, and Hag correlated with reduced carriage. The results support further investigation of these vaccine candidates in protecting against Mcat colonization and infection.

show abstract

Section: Discussionsupporting

confidence: 41%

Serum antibody response to Moraxella catarrhalis proteins OMP CD, OppA, Msp22, Hag, and PilA2 after nasopharyngeal colonization and acute otitis media in children

Ren

Almudevar

Murphy

et al. 2015

Vaccine

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, a recent report indicates a nonsignificant increase in MEF IgA levels against the pneumococcal IgA-1 protease protein in S. pneumoniae-negative/COME-positive children, which may indicate a role in improving the binding of IgA to improve bacterial clearance. This study also reported evidence of a positive correlation between antigen-specific serum and MEE IgG levels for both M. catarrhalis and S. pneumoniae antibodies, which suggests that the antibody response in the middle ear is derived from both the systemic circulation and local mucosal tissue in the middle ear (Verhaegh et al, 2012).…”

Section: Secretory Immunoglobulin a In The Middle Earsupporting

confidence: 61%

Middle Ear and Eustachian Tube Mucosal Immunology

et al. 2015

View full text Add to dashboard Cite

“…In this report we describe the development and careful laboratory validation of a bead based multiplex immunoassay for the simultaneous evaluation of the presence of serum antibodies against S. pneumoniae, H. influenzae, and M. catarrhalis using protein antigens, including several vaccine candidates. Although the use of multiplex assays using pneumococcal proteins has been reported previously (Lebon et al, 2011;Prevaes et al, 2012;Verhaegh et al, 2012), the description of such assays has only been partially published (Shoma et al, 2011), and no data on aspects such as the specificity and comparability with the other assays is available so far. Additionally, this is the first study reporting the validation of a multiplex assay for this specific set of pathogens causing respiratory tract disease, including the information on the possible interference of heterologous inhibition (Prevaes et al, 2012;Verhaegh et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

A fluorescent multiplexed bead-based immunoassay (FMIA) for quantitation of IgG against Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis protein antigens

Andrade

Borges

Laitinen

et al. 2014

Journal of Immunological Methods

View full text Add to dashboard Cite

Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis are pathogens commonly associated with infectious diseases in childhood. This study aimed to develop a fluorescent multiplexed bead-based immunoassay (FMIA) using recombinant proteins for the quantitation of serum IgG antibodies against these bacteria. Eight pneumococcal proteins (Ply, CbpA, PspA1, PspA2, PcpA, PhtD, SP1732-3 and SP2216-1), 3 proteins of H. influenzae (NTHi Protein D, NTHi0371-1, NTHi0830), and 5 proteins of M. catarrhalis (MC Omp CD, MC_RH4_2506, MC_RH4_1701, MC_RH4_3729-1, MC_RH4_4730) were used to develop the FMIA. Optimal coupling concentrations for each protein, comparison of singleplex and multiplex assays, specificity, reproducibility, and correlation to ELISA for six pneumococcal antigens were determined for validation. FMIA was then used to analyze acute and convalescent paired serum samples of 50 children with non-severe pneumonia. The coupling concentrations varied for different antigens, ranging from 1.6 to 32μg of protein/million beads. Correlation between singleplexed and multiplexed assays was excellent, with R≥0.987. The FMIA was specific, reaching >92% homologous inhibition for all specificities; heterologous inhibition ≥20% was found only in six cases. The assay was repeatable, with averages of intra-assay variation ≤10.5%, day-to-day variation ≤9.7% and variation between technicians ≤9.1%. Comparison with ELISA for pneumococcal antigens demonstrated good correlation with R ranging from 0.854 (PspA2) to 0.976 (PcpA). The samples from children showed a wide range of antibody concentrations and increases in convalescent samples. In conclusion, the FMIA was sensitive, specific, and repeatable, using small amounts of recombinant proteins and sera to detect antibodies against S. pneumoniae, H. influenzae and M. catarrhalis. The methodology would be suitable for studies investigating etiological diagnosis and in experimental vaccine studies.

show abstract

Comparative Analysis of the Humoral Immune Response to Moraxella catarrhalis and Streptococcus pneumoniae Surface Antigens in Children Suffering from Recurrent Acute Otitis Media and Chronic Otitis Media with Effusion

Cited by 12 publications

References 40 publications

Serum antibody response to Moraxella catarrhalis proteins OMP CD, OppA, Msp22, Hag, and PilA2 after nasopharyngeal colonization and acute otitis media in children

Serum antibody response to Moraxella catarrhalis proteins OMP CD, OppA, Msp22, Hag, and PilA2 after nasopharyngeal colonization and acute otitis media in children

Middle Ear and Eustachian Tube Mucosal Immunology

A fluorescent multiplexed bead-based immunoassay (FMIA) for quantitation of IgG against Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis protein antigens

Contact Info

Product

Resources

About