Comparative analysis of the ligninolytic potential of basidiomycetes belonging to different taxonomic and ecological groups

The Trametes hirsuta 072 laccase multigene family: Genes identification and transcriptional analysis under copper ions induction

“…Phylogenetic analysis, as well as a comparative analysis of biochemical properties and structure of the substrate-binding pockets of laccases from different clusters of Trametes genus, described in this paper, suggests that laccases of each cluster possess similar physiological functions in different basidiomycetes. Thus, the variability and formation of the sub-clades in the major clusters, apparently can be associated with a geographical origin of particular basidiomycetous species and with the ecological niches it occupies, which in turn determine the ligninolytic and degradation potential of the fungi [80]. We assume that the five new laccases from T. hirsuta …”

Laccase multigene families in Agaricomycetes

“…Another possible explanation of laccase multiplicity in fungi is related to the use of different isozymes on different stages of wood degradation. All species used in this study are representatives of different ecological niches and, as shown earlier, have different ligninolytic and degradative potential [29]. Primary xylotrophs from Pleurotaceae, Polyporaceae, and Phanerochaetaceae families which inhabit dead or living, but not strongly destroyed, wood have formed the group with the highest lignin degrading potential.…”

Elucidation of the crystal structure ofCoriolopsis caperatalaccase: restoration of the structure and activity of the native enzyme from the T2-depleted form by copper ions

“…The medium was prepared by diluting ale wort with water in a 1:4(v:v) ratio and adding 2% agar. The strain was cultivated in the dark on a shaker at 180 rev min À1 for 14 d at 28 C in 750 ml conical flasks containing 25 ml fungal inoculum and 200 ml glucose-peptone medium with the following composition: 10.0 g l À1 glucose, 3.0 g l À1 peptone, 0.6 g l À1 KH 2 PO 4 , 0.001 g l À1 ZnSO 4 .2H 2 O, 0.4 g l À1 K 2 HPO 4 , 0.0005 g l À1 FeSO 4 .7H 2 O, 0.05 g l À1 MnSO 4 .7H 2 O, 0.5 g l À1 MgSO 4 .7H 2 O, 0.5 g l À1 CaCl 2 , with 0.15 g l À1 CuSO 4 as an inducer of laccase production (Fedorova et al, 2013).…”