Comparative analysis of virulence and resistance profiles ofSalmonellaEnteritidis isolates from poultry meat and foodborne outbreaks in northern Jordan

Abstract

This study was conducted to isolate Salmonella Enteritidis from poultry samples and compare their virulence and antibiotic resistance profiles to S. Enteritidis isolated from outbreaks in northern Jordan. Two hundred presumptive isolates were obtained from 302 raw poultry samples and were subjected to further analysis and confirmation. A phylogenic tree based on 16S rRNA sequencing was constructed and selected isolates representing each cluster were further studied for their virulence in normal adult Swiss white mice. The most virulent strains were isolated from poultry samples and had an LD 50 of 1.55 × 10 (5) CFU, while some of the outbreak isolates were avirulent in mice. Antibiotic resistance profiling revealed that the isolates were resistant to seven of eight antibiotics screened with each isolate resistant to multiple antibiotics (from two to six). Of the poultry isolates, 100%, 88.9%, 77.8%, 66.7%, and 50% showed resistance to nalidixic acid, ciprofloxacin, ampicillin, cephalothin, and cefoperazone, respectively. Two outbreak isolates were sensitive to all tested antibiotics, while 71.4% were resistant to cefoperazone and only 28.6% showed resistance to nalidixic acid. Salmonella outbreak isolates were genetically related to poultry isolates as inferred from the 16S rRNA sequencing, yet were phenotypically different. Although outbreak strains were similar to poultry isolates, when tested in the mouse model, some of the outbreak isolates were highly virulent while others were avirulent. This might be due to a variation in susceptibility of the mouse to different S. Enteritidis isolates.

Bacteriological quality and the prevalence of Salmonella spp. and E. coli O157:H7 in ready‐to‐eat foods from Barbados, WI

“…Enterobacter cloacae and K. oxytoca are not considered significant causes of foodborne diseases with few reporting on their occurrence in foods (Feglo & Sakyi, 2012;Jarvis et al, 2018;Nyenje, Odjadjare, et al, 2012; (Jaradat et al, 2014). In our study, Cronobacter spp.…”

“…Furthermore, in the elderly or immunocompromised, infections due to Cronobacter spp. exposure from foods such as dried meats, legumes, nuts, floured spices have been documented (Jaradat et al, ). In our study, Cronobacter spp.…”

Poultry: A receptacle for non-typhoidal Salmonellae and antimicrobial resistance

“…Studies done in south-east Asian countries, such as Philippines showed a prevalence rate of 4.9%NTS from poultry (16), and Vietnam showed 45.6% (19). Jordan showed the highest prevalence of 66% (20), farm prevalence of Nigeria was 43.6% (21), Japan was 54% (13), Egypt was 14.3% (22), Iraq was 10.39% (23) and the United States of America showed 12.4% (24). The occurrence of Salmonella serovars differed among different states of our country where the investigation was done.…”

Piliç Kanatlarından İzole Edilen Salmonella spp.’nin Antibiyotik Direnci

“…High incidence of resistance against streptomycin, tetracycline, trimethoprim, trimethoprim sulfomethoxazole, nalidixic acid have also been declared from Spain [14] , Vietnam [22] , Brazil [15] , Pakistan [16] , Turkey [17] , China [18] , Albania [19] , Ireland [23] , Jordan [24] . Antimicrobial resistance poses a threat to human health and the food animal reservoir is an important source.…”