Comparative anatomical analysis of dopamine systems in Mus musculus and Peromyscus californicus

Buck, Justin; Manion, Matthew; Zhang, Wenyu; Glasper, Erica R.; Wang, Kuan Hong

doi:10.1007/s00429-022-02497-8

Brain Struct Funct

2022

DOI: 10.1007/s00429-022-02497-8

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Comparative anatomical analysis of dopamine systems in Mus musculus and Peromyscus californicus

Justin Buck

Matthew Manion

Wenyu Zhang

et al.

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“…Historically, studies of anatomical sex differences in the brain initially focused on neural regions strongly associated with reproductive behaviors, such as the hypothalamus [13,15]. Consistently reported in several rodent species, the sexually dimorphic nucleus of the medial preoptic area of the hypothalamus (SDN-MPOA) has a larger volume in males, whereas the anteroventral periventricular nucleus (AVPV) of the hypothalamus contains a greater number of dopaminergic neurons in females [16][17][18][19]. In addition, brain regions in the limbic-hypothalamic sensory pathways, such as the medial amygdala (MEA) and the bed nuclei of the stria terminalis (BNST), show prominent anatomical sex differences [20][21][22][23][24].…”

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A sex difference in mouse dopaminergic projections from the midbrain to basolateral amygdala

2022

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Background Dopaminergic circuits play important roles in the motivational control of behavior and dysfunction in dopaminergic circuits have been implicated in several psychiatric disorders, such as schizophrenia and depression. While these disorders exhibit different incidence rates in men and women, the potential sex differences in the underlying neural circuits are not well-understood. Previous anatomical tracing studies in mammalian species have revealed a prominent circuit projection connecting the dopaminergic midbrain ventral tegmental area (VTA) to the basolateral amygdala (BLA), which is involved in emotional processing and associative learning. However, whether there is any sex difference in this anatomical circuit remains unknown. Methods To study the potential sex differences in the VTA-to-BLA dopaminergic circuit, we injected two viral vectors encoding fluorescent reporters of axons and synaptic boutons (AAV–FLEX–tdTomato and AAV–FLEX–SynaptophysinGFP, respectively) into the VTA of a mouse transgenic driver line (tyrosine hydroxylase promoter-driven Cre, or TH-Cre), which restricts the reporter expression to dopaminergic neurons. We then used confocal fluorescent microscopy to image the distribution and density of dopaminergic axons and synaptic boutons in serial sections of both male and female mouse brain. Results We found that the overall labeling intensity of VTA-to-BLA dopaminergic projections is intermediate among forebrain dopaminergic pathways, significantly higher than the projections to the prefrontal cortex, but lower than the projections to the nucleus accumbens. Within the amygdala areas, dopaminergic axons are concentrated in BLA. Although the size of BLA and the density of dopaminergic axons within BLA are similar between male and female mice, the density of dopaminergic synaptic boutons in BLA is significantly higher in male brain than female brain. Conclusions Our results demonstrate an anatomical sex difference in mouse dopaminergic innervations from the VTA to BLA. This finding may provide a structural foundation to study neural circuit mechanisms underlying sex differences in motivational and emotional behaviors and related psychiatric dysfunctions.

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A sex difference in mouse dopaminergic projections from the midbrain to basolateral amygdala

2022

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Comparative anatomical analysis of dopamine systems in Mus musculus and Peromyscus californicus

Cited by 1 publication

References 52 publications

A sex difference in mouse dopaminergic projections from the midbrain to basolateral amygdala

A sex difference in mouse dopaminergic projections from the midbrain to basolateral amygdala

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