Comparative and functional genomics of the Mycobacterium tuberculosis complex
               
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                  aThis review is based on the 2002 Marjory Stephenson Prize Lecture delivered by the author at the 150th Meeting of the Society for General Microbiology, 9 April 2002.

Cole, Stewart T.

doi:10.1099/00221287-148-10-2919

Cited by 116 publications

(75 citation statements)

References 67 publications

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“…The results of this study demonstrate that all the sequenced products share the consensus sequence, and that there is a high similarity between them with the species M. tuberculosis and M. bovis, which belong to the MTB complex and share the same ancestor in their evolutionary events as previously reported [27,28].…”

Section: Discussionsupporting

confidence: 78%

Evaluation of conventional molecular diagnosis of Mycobacterium tuberculosis in clinical specimens from Morocco

Zakham

Bazoui

Akrim

et al. 2011

J Infect Dev Ctries

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Introduction: Tuberculosis is a major public health threat, annually affecting new individuals worldwide, especially those in developing countries. Rapid detection of the agent and effective treatment are two important factors in controlling this disease. Methodology: The present study aimed to evaluate polymerase chain reaction (PCR) as a rapid and direct molecular method for the diagnosis of Mycobacterium tuberculosis (MTB) in 70 clinical specimens (62 sputum samples, six cerebrospinal fluids, and two biopsies) using heat shock protein (hsp65) as the gene target. Automated sequencing of the same gene was used for the identification of MTB to the species level. Results: The sensitivity of PCR was 81.13%, with specificity of 88.24%; the positive and negative predictive values were 95.56% and 60%, respectively. Conclusion: Based on these results, the hsp65 gene sequence can be used to differentiate the members of MTB complex from nontuberculosis mycobacteria (NTM).

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Section: Discussionsupporting

confidence: 78%

Evaluation of conventional molecular diagnosis of Mycobacterium tuberculosis in clinical specimens from Morocco

Zakham

Bazoui

Akrim

et al. 2011

J Infect Dev Ctries

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“…It was thought that such a panel of strains would show LSPs in a screening of the whole genome. Based on the genomic versatility discovered in other mycobacterial species (5,9,12,34,58), it was commonly assumed that LSPs would provide the key source of diversity, with genetic discriminatory power for microepidemiological purposes. However, no such variation was found across the investigated collection.…”

Section: Discussionmentioning

confidence: 99%

“…Mycobacterium tuberculosis and related pathogenic mycobacteria exhibit major large sequence polymorphism (LSP)-based interstrain genomic variations (5,9,12,34,58). From these genomic insertional-deletional (InDel) markers, evolutionary scenarios were drawn for M. tuberculosis, the M. tuberculosis complex, Mycobacterium bovis, the M. bovis BCG vaccine strains, and mycolactone-producing mycobacteria related to Mycobacterium ulcerans (6-8, 25, 27, 38, 50).…”

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confidence: 99%

Lack of Insertional-Deletional Polymorphism in a Collection of Mycobacterium ulcerans Isolates from Ghanaian Buruli Ulcer Patients

et al. 2009

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Mycobacterium ulcerans causes the devastating infectious skin disease Buruli ulcer and has a monomorphic population structure. The resolution of conventional genetic fingerprinting methods is therefore not sufficient for microepidemiological studies aiming to characterize transmission pathways. In a previous comparative genomic hybridization analysis with a microarray covering part of the M. ulcerans genome, we have found extensive insertional-deletional sequence polymorphisms among M. ulcerans isolates of diverse geographic origins that allowed us to distinguish between strains coming from different continents. Since large numbers of insertion sequences are spread over the genome of African M. ulcerans strains, we reasoned that these may drive large sequence polymorphisms in otherwise clonal local mycobacterial populations. In this study, we used a printed DNA microarray covering the whole genome of the Ghanaian M. ulcerans reference strain Agy99 for comparative genomic hybridization. The assay identified multiple regions of difference when DNA of a Japanese M. ulcerans strain was analyzed. In contrast, not a single insertional-deletional genomic variation was found within a panel of disease isolates coming from an area of Ghana where Buruli ulcer is endemic. These results indicate that, despite the expectations deduced from other mycobacterial pathogens, only analyses of single nucleotide polymorphisms will have the potential to differentiate local populations of M. ulcerans.

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“…Current versions of these assays use two recombinant Mtb Ags, early secretory antigenic target 6 (ESAT-6), and culture filtrate protein 10 (CFP-10). These proteins are encoded within the region of difference 1 of the Mtb genome that is deleted in all attenuated BCG vaccine strains and most nontuberculous mycobacteria (11). High ESAT-6-specific IFN-␥ responses have been positively correlated with pathology (12).…”

mentioning

confidence: 99%

Messenger RNA Expression of IL-8, FOXP3, and IL-12β Differentiates Latent Tuberculosis Infection from Disease

Huang

Kato‐Maeda

et al. 2007

The Journal of Immunology

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Differentiation of active from latent tuberculosis (TB) is a major challenge in the control of TB. In this study, PBMC from latent TB-infected subjects, TB patients, and tuberculin skin test-negative donors stimulated with the Mycobacterium tuberculosis (Mtb)-specific Ag, early secretory antigenic target 6, and mRNA for 45 immune-related genes was measured by quantitative real-time PCR. Univariate analysis showed significant differences in the expression of 10 genes (IFN-γ, FOXP3, IL-1α, IL-1β, IL-2, IL-6, IL-8, IL-12α, IL-12β, and IL-24) in PBMC from TB patients vs latent TB-infected subjects (p < 0.01). Multivariate logistic regression and classification and regression tree analyses revealed that expression of three genes, IL-8, FOXP3, and IL-12β, is predictive for TB vs latent Mtb infection. Thus, measurement of Ag-specific expression of these three genes may offer a specific and noninvasive means of differentiating between latent Mtb infection and TB.

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Comparative and functional genomics of the Mycobacterium tuberculosis complex a aThis review is based on the 2002 Marjory Stephenson Prize Lecture delivered by the author at the 150th Meeting of the Society for General Microbiology, 9 April 2002.

Cited by 116 publications

References 67 publications

Evaluation of conventional molecular diagnosis of Mycobacterium tuberculosis in clinical specimens from Morocco

Evaluation of conventional molecular diagnosis of Mycobacterium tuberculosis in clinical specimens from Morocco

Lack of Insertional-Deletional Polymorphism in a Collection of Mycobacterium ulcerans Isolates from Ghanaian Buruli Ulcer Patients

Messenger RNA Expression of IL-8, FOXP3, and IL-12β Differentiates Latent Tuberculosis Infection from Disease

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