Comparative antioxidant, antibacterial, and GC-MS analysis of methanol extract’s fractions and isolation of luteolin from leaves of trans-Himalayan Codonopsis clematidea

Bhardwaj, Pushpender; Naryal, Avilekh; Thakur, Mayank; Aggarwal, Neeraj; Saxena, Shweta; Chaurasia, O. P.; Kumar, Raj

doi:10.1016/j.indcrop.2019.112046

Cited by 20 publications

(13 citation statements)

References 33 publications

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“…The result suggests that the effects shown by EF, AF and BF on ABTS radicals could prevent or ameliorate the oxidative damages. The observations of our study corroborates with the study on Codonopsis clematidea and Ficus microcarpa L. which revealed an identical performance pattern of EF among all the other fractions 30,40 . The results of FRAP assay coincide very well with earlier reports by other authors where FRAP assay was observed to show analogous results to DPPH and ABTS 30,41 .…”

Section: Discussionsupporting

confidence: 91%

“…According to the results obtained, the most polar fractions give a high yield. A similar course of results was found in case of Codonopsis clematidea [30] where the polar fraction (BF) has maximum yield than other fractions. On the other hand according to the results obtained by Nsimba et al 31 , they found that the non-polar fraction (HF) of Chenopodium quinoa and Amaranthus cruentus has a maximum yield compared to other extracts.…”

Section: Discussionsupporting

confidence: 80%

“…This suggests that antioxidant activity could be the attribute of the phenolic and flavonoid content of these fractions. An identical trend was observed in the case of Achillea millefolium, Foeniculum vulgare, Lavandula latifolia 35 and Codonopsis clematidea 30 where EF showed a higher value than the other fractions. In addition, the authors reported such a relationship between the antioxidant with PTC and TFC 36 .…”

Section: Discussionsupporting

confidence: 78%

“…However, this may be attributed due to condensing the active compounds in these two fractions and removal of undesirable components. An equivalent result was reported in case of Lysimachia foenum-graecum 34 and Codonopsis clematidea 30 where EF has shown better IC50 in comparison to the other fractions.…”

Section: Discussionsupporting

confidence: 74%

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Antioxidant Evaluation and Polyphenol Contents of Hydro Ethanolic Extract’s Fractions from Ephedra nebrodensis

Hamoudi¹,

Amroun²,

Khennouf³

et al. 2020

J. Drug Delivery Ther.

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Ephedra nebrodensis, is a medicinal plant, traditionally in Algeria used for the cure of various ailments such as hepatic disorders. On this basis in present study the phytochemical screening and in vitro antioxidant activity of various fractions (n-hexane, chloroform, ethyl acetate, n-butanol and water) of ethanol extract obtained by maceration from aerial parts of E. nebrodensis were investigated. Different fractions were accessed for their yield percentage, total polyphenol content (TPC), total flavonoid content (TFC), total tannins content (TTC) and antioxidant potential (DPPH - 2,2-diphenyl-1-picrylhydrazyl; ABTS - 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid), reducing power assay (FRAP - ferric reducing antioxidant power activity),and bleaching of β-carotene test . Results showed that ethyl acetate fraction (EF) represents the highest amount of total polyphenols, flavonoids and tannins with 269.669 ± 0.031 µg gallic acid equivalent/mg of DE, 44.507 ± 0.003 µg quercetin equivalents/mg of DE and 228.487 ± 1.362 µg tannic acid equivalents/mg of DE, respectively. In antioxidant assays, ethyl acetate fractions (EF) showed the strongest DPPH and ABTS antioxidant (IC50: 0.009 and 0.004 µg/mL) and FRAP potential (EC50: 0.005 ± 0.000 µg/mL), which significantly correlate to its high content of polyphenolics, flavonoids and tannins. Further, fractions were rich in phenolic compounds. The present results support the possible use of the ethyl acetate fraction from E. nebrodensis in pharmaceutical industries as a natural antioxidant. Keywords: Ephedra nebrodensis, polyphenols, flavonoids, tannins, antioxidant capacity.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 78%

Section: Discussionsupporting

confidence: 74%

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Antioxidant Evaluation and Polyphenol Contents of Hydro Ethanolic Extract’s Fractions from Ephedra nebrodensis

Hamoudi¹,

Amroun²,

Khennouf³

et al. 2020

J. Drug Delivery Ther.

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“…Our data concerning TP and TF contents are consistent to those of Yao et al [ 27 ] who report that the EAF obtained from the medicinal plant Pyrola asarifolia had the highest levels of phenolics and flavonoids compared to the other fractions (petroleum ether, n-butanol, and water). In their study, Bhardwaj et al [ 28 ] also reported similar results when using several solvents with increasing polarity (n-hexane, chloroform, ethyl acetate, and n-butanol) in the splitting of a medicinal plant Codonopsis clematidea. According to these authors, the EAF has the highest TP and TF contents distantly followed by the n-butanol, chloroform, and hexane ones.…”

Section: Resultsmentioning

confidence: 63%

Bioactive Compounds from Ephedra fragilis: Extraction Optimization, Chemical Characterization, Antioxidant and AntiGlycation Activities

et al. 2021

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Response surface methodology (RSM) with a Box–Behnken design (BBD) was used to optimize the extraction of bioactive compounds from Ephedra fragilis. The results suggested that extraction with 61.93% ethanol at 44.43 °C for 15.84 h was the best solution for this combination of variables. The crude ethanol extract (CEE) obtained under optimum extraction conditions was sequentially fractionated with solvents of increasing polarity. The content of total phenolic (TP) and total flavonoid (TF) as well as the antioxidant and antiglycation activities were measured. The phytochemical fingerprint profile of the fraction with the highest activity was characterized by using RP-HPLC. The ethyl acetate fraction (EAF) had the highest TP and TF contents and exhibited the most potent antioxidant and antiglycation activities. The Pearson correlation analysis results showed that TP and TF contents were highly significantly correlated with the antioxidant and antiglycation activities. Totally, six compounds were identified in the EAF of E. fragilis, including four phenolic acids and two flavonoids. Additionally, molecular docking analysis also showed the possible connection between identified bioactive compounds and their mechanisms of action. Our results suggest new evidence on the antioxidant and antiglycation activities of E. fragilis bioactive compounds that may be applied in the treatment and prevention of aging and glycation-associated complications.

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Analysis of active compounds and bioactivity of leaves extracts of Sonneratia species

Nguyen,

Nguyen

et al. 2024

Engineering Reports

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The present study focused on the quantitative determination of active compounds, antioxidant and antibacterial activities of leaf extracts of different Sonneratia species. The tested species including Sonneratia alba (SA), Sonneratia caseolaris (SC), and Sonneratia ovata Backer (SO) were collected from Can Gio Mangrove Forest, Vietnam. The methanol extract of SA leaves was found to contain the highest content of total flavonoids, total phenolics and gallic acid (4344.16 ± 51.78 μg/g dry mass). The highest luteolin‐7‐O‐glucoside and luteolin amounts (5963.28 ± 59.77 and 234.33 ± 10.41 μg/g dry mass, respectively) were found in the methanol extract of SC leaves according to HPLC‐DAD analysis. The methanol extracts of all three species exhibited strong DPPH radical scavenging activity while the ethanol extracts provided strong antibacterial activity against Staphylococcus aureus, Salmonella typhimurium, and Escherichia coli. By fractionating the extracts over a SiO2 column using CHCl3: EA: MeOH gradients, the fractions containing high purity luteolin and luteolin‐7‐glucoside from SC and high purity luteolin from SO were collected. Moreover, all fractions demonstrated significant DPPH free radical scavenging ability but the fraction enriched in gallic acid showed the highest activity. These findings show that SA is a promising source of gallic acid while SC can be potentially used for the extraction and isolation of luteolin‐7‐glucoside.

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Comparative antioxidant, antibacterial, and GC-MS analysis of methanol extract’s fractions and isolation of luteolin from leaves of trans-Himalayan Codonopsis clematidea

Cited by 20 publications

References 33 publications

Antioxidant Evaluation and Polyphenol Contents of Hydro Ethanolic Extract’s Fractions from Ephedra nebrodensis

Antioxidant Evaluation and Polyphenol Contents of Hydro Ethanolic Extract’s Fractions from Ephedra nebrodensis

Bioactive Compounds from Ephedra fragilis: Extraction Optimization, Chemical Characterization, Antioxidant and AntiGlycation Activities

Analysis of active compounds and bioactivity of leaves extracts of Sonneratia species

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