Comparative assessment of in vitro antidiabetic activity of Tinospora cordifolia stem, leaves and its callus extracts

Abstract

Callus can be a viable alternative to obtain important phytochemicals and analyze crude extract for pharmacological activities rather than going the cumbersome way of collecting and destroying possibly endangered plants. In this study, callus was produced using stem and leaf explants of Tinospora cordifolia, and methanol extract of stem leaf, stem callus and leaf callus were evaluated for their antidiabetic potential. Inhibition of glycosylation of haemoglobin and α-amylase inhibition was in a dose dependent manner and glucose transport differs with the sample and glucose concentration. The results of the work indicate that the bot h native plant extracts and callus extracts possessed considerable in vitro anti diabetic activity and can be applied as alternative in the treatment of diabetes and diabetic induced complication.