Callus can be a viable alternative to obtain important phytochemicals and analyze crude extract for pharmacological activities rather than going the cumbersome way of collecting and destroying possibly endangered plants. In this study, callus was produced using stem and leaf explants of Tinospora cordifolia, and methanol extract of stem leaf, stem callus and leaf callus were evaluated for their antidiabetic potential. Inhibition of glycosylation of haemoglobin and α-amylase inhibition was in a dose dependent manner and glucose transport differs with the sample and glucose concentration. The results of the work indicate that the bot h native plant extracts and callus extracts possessed considerable in vitro anti diabetic activity and can be applied as alternative in the treatment of diabetes and diabetic induced complication.

The present study deals with the in vitro anti-inflammatory in methanolic extracts of in vivo (leaf and stem) and in vitro (callus) plant parts of Tinospora cordifolia (Acanthaceae) is a medicinal plant of immense therapeutic value. The present work is based on developing a protocol for the callus induction in T. cordifolia from stem and leaf explants. The highest rate of callus (70.82%) was observed on MS medium fortified with 1.5 mg/L 2, 4-D and 0.3 mg/L BAP. The methanolic extracts of all plant parts exhibited remarkable anti-inflammatory activity action. The maximum amount of membrane stabilization was found in stem callus (78.15% at a dose of 100μg/ml) as compare to other plant and callus parts tested. Therefore, our studies support the isolation and the use of active constituents from in vivo and in vitro plant parts of T. cordifolia in treating inflammations.